Construct: ORF ccsbBroad304_06567

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010180.1_s304c1, BRDN0000404902
Derived from:
ccsbBroadEn_06567
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCAM (4162)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06567
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4162 MCAM melanoma cell adhesion mole... NM_006500.3 99.9% 100% 1800A>G
2 human 4162 MCAM melanoma cell adhesion mole... XM_017017759.2 96.6% 96.7% 1485_1486ins63;1737A>G
3 human 4162 MCAM melanoma cell adhesion mole... XM_017017760.2 94.9% 95% 1548_1549ins96;1704A>G
4 human 4162 MCAM melanoma cell adhesion mole... XM_017017761.2 93.4% 92.5% 1793_1794ins118;1812_1813insTGAGGCAT
5 human 4162 MCAM melanoma cell adhesion mole... XM_017017762.2 90.2% 89.3% 1485_1486ins63;1730_1731ins118;1749_1750insTGAGGCAT
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2004
ORF length:
1938
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg gcttcccagg ctggtctgcg ccttcttgct cgccgcctgc tgctgctgtc
121ctcgcgtcgc gggtgtgccc ggagaggctg agcagcctgc gcctgagctg gtggaggtgg
181aagtgggcag cacagccctt ctgaagtgcg gcctctccca gtcccaaggc aacctcagcc
241atgtcgactg gttttctgtc cacaaggaga agcggacgct catcttccgt gtgcgccagg
301gccagggcca gagcgaacct ggggagtacg agcagcggct cagcctccag gacagagggg
361ctactctggc cctgactcaa gtcacccccc aagacgagcg catcttcttg tgccagggca
421agcgccctcg gtcccaggag taccgcatcc agctccgcgt ctacaaagct ccggaggagc
481caaacatcca ggtcaacccc ctgggcatcc ctgtgaacag taaggagcct gaggaggtcg
541ctacctgtgt agggaggaac gggtacccca ttcctcaagt catctggtac aagaatggcc
601ggcctctgaa ggaggagaag aaccgggtcc acattcagtc gtcccagact gtggagtcga
661gtggtttgta caccttgcag agtattctga aggcacagct ggttaaagaa gacaaagatg
721cccagtttta ctgtgagctc aactaccggc tgcccagtgg gaaccacatg aaggagtcca
781gggaagtcac cgtccctgtt ttctacccga cagaaaaagt gtggctggaa gtggagcccg
841tgggaatgct gaaggaaggg gaccgcgtgg aaatcaggtg tttggctgat ggcaaccctc
901caccacactt cagcatcagc aagcagaacc ccagcaccag ggaggcagag gaagagacaa
961ccaacgacaa cggggtcctg gtgctggagc ctgcccggaa ggaacacagt gggcgctatg
1021aatgtcaggg cctggacttg gacaccatga tatcgctgct gagtgaacca caggaactac
1081tggtgaacta tgtgtctgac gtccgagtga gtcccgcagc ccctgagaga caggaaggca
1141gcagcctcac cctgacctgt gaggcagaga gtagccagga cctcgagttc cagtggctga
1201gagaagagac aggccaggtg ctggaaaggg ggcctgtgct tcagttgcat gacctgaaac
1261gggaggcagg aggcggctat cgctgcgtgg cgtctgtgcc cagcataccc ggcctgaacc
1321gcacacagct ggtcaacgtg gccatttttg gccccccttg gatggcattc aaggagagga
1381aggtgtgggt gaaagagaat atggtgttga atctgtcttg tgaagcgtca gggcaccccc
1441ggcccaccat ctcctggaac gtcaacggca cggcaagtga acaagaccaa gatccacagc
1501gagtcctgag caccctgaat gtcctcgtga ccccggagct gttggagaca ggtgttgaat
1561gcacggcctc caacgacctg ggcaaaaaca ccagcatcct cttcctggag ctggtcaatt
1621taaccaccct cacaccagac tccaacacaa ccactggcct cagcacttcc actgccagtc
1681ctcataccag agccaacagc acctccacag agagaaagct gccggagccg gagagccggg
1741gcgtggtcat cgtggctgtg attgtgtgca tcctggtcct ggcggtgctg ggcgctgtcc
1801tctatttcct ctataagaag ggcaagctgc cgtgcaggcg ctcagggaag caggagatca
1861cgctgccccc gtctcgtaag agcgaacttg tagttgaagt taagtcagat aagctcccag
1921aagagatggg cctcctgcag ggcagcagcg gtgacaagag ggctccggga gaccagggag
1981agaaatacat cgatctgagg cattacccaa ctttcttgta caaagtggtt ggtaagccta
2041tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
2101gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)