Construct: ORF ccsbBroad304_06580

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004789.1_s304c1, BRDN0000400082
Derived from:
ccsbBroadEn_06580
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGAT1 (4245)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06580
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114617.1 99.9% 99.7% 1304T>C
2 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114618.1 99.9% 99.7% 1304T>C
3 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114619.1 99.9% 99.7% 1304T>C
4 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114620.1 99.9% 99.7% 1304T>C
5 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364377.2 99.9% 99.7% 1304T>C
6 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364379.2 99.9% 99.7% 1304T>C
7 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364380.2 99.9% 99.7% 1304T>C
8 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364381.2 99.9% 99.7% 1304T>C
9 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364382.2 99.9% 99.7% 1304T>C
10 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364383.2 99.9% 99.7% 1304T>C
11 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364384.2 99.9% 99.7% 1304T>C
12 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364385.2 99.9% 99.7% 1304T>C
13 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364386.2 99.9% 99.7% 1304T>C
14 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364387.2 99.9% 99.7% 1304T>C
15 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364388.2 99.9% 99.7% 1304T>C
16 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364389.2 99.9% 99.7% 1304T>C
17 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364390.2 99.9% 99.7% 1304T>C
18 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364391.2 99.9% 99.7% 1304T>C
19 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364392.2 99.9% 99.7% 1304T>C
20 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364393.2 99.9% 99.7% 1304T>C
21 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364394.2 99.9% 99.7% 1304T>C
22 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364395.2 99.9% 99.7% 1304T>C
23 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_002406.4 99.9% 99.7% 1304T>C
24 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110148.1 84.3% 89.9% (many diffs)
25 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110149.1 84.3% 89.9% (many diffs)
26 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110150.1 84.3% 89.9% (many diffs)
27 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_010794.3 84.3% 89.9% (many diffs)
28 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532397.3 84.3% 89.9% (many diffs)
29 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532398.3 84.3% 89.9% (many diffs)
30 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532399.2 84.3% 89.9% (many diffs)
31 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532400.3 84.3% 89.9% (many diffs)
32 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_017314314.1 84.3% 89.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1401
ORF length:
1335
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gaagaagcag tctgcagggc ttgtgctgtg gggcgctatc ctctttgtgg
121cctggaatgc cctgctgctc ctcttcttct ggacgcgccc agcacctggc aggccaccct
181cagtcagcgc tctcgatggc gaccccgcca gcctcacccg ggaagtgatt cgcctggccc
241aagacgccga ggtggagctg gagcggcagc gtgggctgct gcagcagatc ggggatgccc
301tgtcgagcca gcgggggagg gtgcccaccg cggcccctcc cgcccagccg cgtgtgcctg
361tgacccccgc gccggcggtg attcccatcc tggtcatcgc ctgtgaccgc agcactgttc
421ggcgctgcct ggacaagctg ctgcattatc ggccctcggc tgagctcttc cccatcatcg
481ttagccagga ctgcgggcac gaggagacgg cccaggccat cgcctcctac ggcagcgcgg
541tcacgcacat ccggcagccc gacctgagca gcattgcggt gccgccggac caccgcaagt
601tccagggcta ctacaagatc gcgcgccact accgctgggc gctgggccag gtcttccggc
661agtttcgctt ccccgcggcc gtggtggtgg aggatgacct ggaggtggcc ccggacttct
721tcgagtactt tcgggccacc tatccgctgc tgaaggccga cccctccctg tggtgcgtct
781cggcctggaa tgacaacggc aaggagcaga tggtggacgc cagcaggcct gagctgctct
841accgcaccga ctttttccct ggcctgggct ggctgctgtt ggccgagctc tgggctgagc
901tggagcccaa gtggccaaag gccttctggg acgactggat gcggcggccg gagcagcggc
961aggggcgggc ctgcatacgc cctgagatct caagaacgat gacctttggc cgcaagggtg
1021tgagccacgg gcagttcttt gaccagcacc tcaagtttat caagctgaac cagcagtttg
1081tgcacttcac ccagctggac ctgtcttacc tgcagcggga ggcctatgac cgagatttcc
1141tcgcccgcgt ctacggtgct ccccagctgc aggtggagaa agtgaggacc aatgaccgga
1201aggagctggg ggaggtgcgg gtgcagtata cgggcaggga cagcttcaag gctttcgcca
1261aggctctggg tgtcatggat gaccttaagt cgggggttcc gagagctggc taccggggta
1321ttgtcacctt ccagttccgg ggccgccgtg tccacctggc gcccccaccg acgtgggagg
1381gctatgatcc tagctggaat taccaacttt cttgtacaaa gtggttggta agcctatccc
1441taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt
1501taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)