Construct: ORF ccsbBroad304_06591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016378.1_s304c1, BRDN0000407570
- Derived from:
- ccsbBroadEn_06591
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MMP9 (4318)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06591
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4318 | MMP9 | matrix metallopeptidase 9 | NM_004994.3 | 99.9% | 99.7% | 836A>G;1721G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cctctggcag cccctggtcc tggtgctcct ggtgctgggc tgctgctttg 121 ctgcccccag acagcgccag tccacccttg tgctcttccc tggagacctg agaaccaatc 181 tcaccgacag gcagctggca gaggaatacc tgtaccgcta tggttacact cgggtggcag 241 agatgcgtgg agagtcgaaa tctctggggc ctgcgctgct gcttctccag aagcaactgt 301 ccctgcccga gaccggtgag ctggatagcg ccacgctgaa ggccatgcga accccacggt 361 gcggggtccc agacctgggc agattccaaa cctttgaggg cgacctcaag tggcaccacc 421 acaacatcac ctattggatc caaaactact cggaagactt gccgcgggcg gtgattgacg 481 acgcctttgc ccgcgccttc gcactgtgga gcgcggtgac gccgctcacc ttcactcgcg 541 tgtacagccg ggacgcagac atcgtcatcc agtttggtgt cgcggagcac ggagacgggt 601 atcccttcga cgggaaggac gggctcctgg cacacgcctt tcctcctggc cccggcattc 661 agggagacgc ccatttcgac gatgacgagt tgtggtccct gggcaagggc gtcgtggttc 721 caactcggtt tggaaacgca gatggcgcgg cctgccactt ccccttcatc ttcgagggcc 781 gctcctactc tgcctgcacc accgacggtc gctccgacgg cttgccctgg tgcagtacca 841 cggccaacta cgacaccgac gaccggtttg gcttctgccc cagcgagaga ctctacaccc 901 gggacggcaa tgctgatggg aaaccctgcc agtttccatt catcttccaa ggccaatcct 961 actccgcctg caccacggac ggtcgctccg acggctaccg ctggtgcgcc accaccgcca 1021 actacgaccg ggacaagctc ttcggcttct gcccgacccg agctgactcg acggtgatgg 1081 ggggcaactc ggcgggggag ctgtgcgtct tccccttcac tttcctgggt aaggagtact 1141 cgacctgtac cagcgagggc cgcggagatg ggcgcctctg gtgcgctacc acctcgaact 1201 ttgacagcga caagaagtgg ggcttctgcc cggaccaagg atacagtttg ttcctcgtgg 1261 cggcgcatga gttcggccac gcgctgggct tagatcattc ctcagtgccg gaggcgctca 1321 tgtaccctat gtaccgcttc actgaggggc cccccttgca taaggacgac gtgaatggca 1381 tccggcacct ctatggtcct cgccctgaac ctgagccacg gcctccaacc accaccacac 1441 cgcagcccac ggctcccccg acggtctgcc ccaccggacc ccccactgtc cacccctcag 1501 agcgccccac agctggcccc acaggtcccc cctcagctgg ccccacaggt ccccccactg 1561 ctggcccttc tacggccact actgtgcctt tgagtccggt ggacgatgcc tgcaacgtga 1621 acatcttcga cgccatcgcg gagattggga accagctgta tttgttcaag gatgggaagt 1681 actggcgatt ctctgagggc agggggagcc ggccgcaggg ccccttcctt atcgccgaca 1741 agtggcccgc gctgccccgc aagctggact cggtctttga ggagccgctc tccaagaagc 1801 ttttcttctt ctctgggcgc caggtgtggg tgtacacagg cgcgtcggtg ctgggcccga 1861 ggcgtctgga caagctgggc ctgggagccg acgtggccca ggtgaccggg gccctccgga 1921 gtggcagggg gaagatgctg ctgttcagcg ggcggcgcct ctggaggttc gacgtgaagg 1981 cgcagatggt ggatccccgg agcgccagcg aggtggaccg gatgttcccc ggggtgcctt 2041 tggacacgca cgacgtcttc cagtaccgag agaaagccta tttctgccag gaccgcttct 2101 actggcgcgt gagttcccgg agtgagttga accaggtgga ccaagtgggc tacgtgacct 2161 atgacatcct gcagtgccct gaggactacc caactttctt gtacaaagtg gttggtaagc 2221 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2281 ggcgcgttaa gtcgacaatc aacctctgga tta