Construct: ORF ccsbBroad304_06612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007976.1_s304c1, BRDN0000400138
Derived from:
ccsbBroadEn_06612
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYOC (4653)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06612
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4653 MYOC myocilin NM_000261.2 99.9% 99.8% 7T>A
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1578
ORF length:
1512
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gatcttctgt gcacgttgct gcagctttgg gcctgagatg ccagctgtcc
121agctgctgct tctggcctgc ctggtgtggg atgtgggggc caggacagct cagctcagga
181aggccaatga ccagagtggc cgatgccagt ataccttcag tgtggccagt cccaatgaat
241ccagctgccc agagcagagc caggccatgt cagtcatcca taacttacag agagacagca
301gcacccaacg cttagacctg gaggccacca aagctcgact cagctccctg gagagcctcc
361tccaccaatt gaccttggac caggctgcca ggccccagga gacccaggag gggctgcaga
421gggagctggg caccctgagg cgggagcggg accagctgga aacccaaacc agagagttgg
481agactgccta cagcaacctc ctccgagaca agtcagttct ggaggaagag aagaagcgac
541taaggcaaga aaatgagaat ctggccagga ggttggaaag cagcagccag gaggtagcaa
601ggctgagaag gggccagtgt ccccagaccc gagacactgc tcgggctgtg ccaccaggct
661ccagagaagt ttctacgtgg aatttggaca ctttggcctt ccaggaactg aagtccgagc
721taactgaagt tcctgcttcc cgaattttga aggagagccc atctggctat ctcaggagtg
781gagagggaga caccggatgt ggagaactag tttgggtagg agagcctctc acgctgagaa
841cagcagaaac aattactggc aagtatggtg tgtggatgcg agaccccaag cccacctacc
901cctacaccca ggagaccacg tggagaatcg acacagttgg cacggatgtc cgccaggttt
961ttgagtatga cctcatcagc cagtttatgc agggctaccc ttctaaggtt cacatactgc
1021ctaggccact ggaaagcacg ggtgctgtgg tgtactcggg gagcctctat ttccagggcg
1081ctgagtccag aactgtcata agatatgagc tgaataccga gacagtgaag gctgagaagg
1141aaatccctgg agctggctac cacggacagt tcccgtattc ttggggtggc tacacggaca
1201ttgacttggc tgtggatgaa gcaggcctct gggtcattta cagcaccgat gaggccaaag
1261gtgccattgt cctctccaaa ctgaacccag agaatctgga actcgaacaa acctgggaga
1321caaacatccg taagcagtca gtcgccaatg ccttcatcat ctgtggcacc ttgtacaccg
1381tcagcagcta cacctcagca gatgctaccg tcaactttgc ttatgacaca ggcacaggta
1441tcagcaagac cctgaccatc ccattcaaga accgctataa gtacagcagc atgattgact
1501acaaccccct ggagaagaag ctctttgcct gggacaactt gaacatggtc acttatgaca
1561tcaagctctc caagatgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
1621accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1681agtcgacaat caacctctgg atta

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