Construct: ORF ccsbBroad304_06624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010986.1_s304c1, BRDN0000403687
- Derived from:
- ccsbBroadEn_06624
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFC2 (4718)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06624
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4718 | NDUFC2 | NADH:ubiquinone oxidoreduct... | NM_004549.6 | 99.7% | 100% | 138A>G |
| 2 | human | 100532726 | NDUFC2-KCTD14 | NDUFC2-KCTD14 readthrough | NM_001203261.2 | 87.5% | 84.6% | (many diffs) |
| 3 | human | 4718 | NDUFC2 | NADH:ubiquinone oxidoreduct... | NM_001204055.2 | 80.3% | 80.6% | 138A>G;165_166ins69 |
| 4 | human | 100532726 | NDUFC2-KCTD14 | NDUFC2-KCTD14 readthrough | NM_001203260.2 | 78.9% | 71.9% | (many diffs) |
| 5 | human | 4718 | NDUFC2 | NADH:ubiquinone oxidoreduct... | NM_001204054.2 | 68.6% | 66.3% | (many diffs) |
| 6 | human | 100532726 | NDUFC2-KCTD14 | NDUFC2-KCTD14 readthrough | NM_001203262.2 | 57.4% | 47.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat cgcacggcgg aacccagaac ccttacggtt tctgccggat gaggcccgga 121 gcctgccccc gcccaagctg accgacccgc ggctcctcta catcggcttc ttgggctact 181 gctccggcct gattgataac ctgatccggc ggaggccgat cgcgacggct ggtttgcatc 241 gccagcttct atatattacg gccttttttt ttgctggata ttatcttgta aaacgtgaag 301 actacctgta tgctgtgagg gaccgtgaaa tgtttggata tatgaaatta catccagagg 361 attttcctga agaagataag aaaacatatg gtgaaatttt tgaaaaattc catccaatac 421 gttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta