Construct: ORF ccsbBroad304_06625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007240.1_s304c1, BRDN0000400524
- Derived from:
- ccsbBroadEn_06625
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFS4 (4724)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06625
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4724 | NDUFS4 | NADH:ubiquinone oxidoreduct... | NM_002495.4 | 99.2% | 99.4% | (many diffs) |
| 2 | human | 4724 | NDUFS4 | NADH:ubiquinone oxidoreduct... | NR_134474.2 | 68.7% | (many diffs) | |
| 3 | human | 4724 | NDUFS4 | NADH:ubiquinone oxidoreduct... | NM_001318051.2 | 67.4% | 66.2% | (many diffs) |
| 4 | human | 4724 | NDUFS4 | NADH:ubiquinone oxidoreduct... | NR_134475.2 | 65.6% | (many diffs) | |
| 5 | human | 4724 | NDUFS4 | NADH:ubiquinone oxidoreduct... | NR_134473.2 | 61.9% | (many diffs) | |
| 6 | mouse | 17993 | Ndufs4 | NADH dehydrogenase (ubiquin... | NM_010887.2 | 88% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggtctca atgtcagtgg tactgaggca gacgttgtgg cggagaaggg 121 cagtggctgt agctgccctt tccgtttcca gggttccgac caggtcgttg aggacttcct 181 catggagatt ggcacaggac cagactcaag acacacaact cataacagtt gatgaaaaat 241 tggatatcac tactttaact ggcgttccag aagagcatat aaaaactaga aaagtcagga 301 tctttgttcc tgctcgcaat aacatgcagt ctggagtaaa caacacaaag aaatggaaga 361 tggagtttga taccagggag cgatgggaaa atcctttgat gggttgggca tcaacggctg 421 atcccttatc caacatggtt ctaaccttca gtactaaaga agatgcagtt tcctttgcag 481 aaaaaaatgg atggagctat gacattgaag agaggaaggt tccaaaaccc aagtccaagt 541 cttatggtgc aaacttttct tggaacaaaa gaacaagagt atccacaaaa tacccaactt 601 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 661 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta