Construct: ORF ccsbBroad304_06651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010610.1_s304c1, BRDN0000398955
- Derived from:
- ccsbBroadEn_06651
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCN3 (4856)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06651
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4856 | CCN3 | cellular communication netw... | NM_002514.4 | 99.9% | 99.7% | 291C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gagtgtgcag agcacgagct tttgtctccg aaagcagtgc ctttgcctga 121 ccttcctgct tctccatctc ctgggacagg tcgctgcgac tcagcgctgc cctccccagt 181 gcccgggccg gtgccctgcg acgccgccga cctgcgcccc cggggtgcgc gcggtgctgg 241 acggctgctc atgctgtctg gtgtgtgccc gccagcgtgg cgagagctgc tcagatctgg 301 agccatgcga cgagagcagt ggcctctact gtgatcgcag cgcggacccc agcaaacaga 361 ctggcatctg cacggcggta gagggagata actgtgtgtt cgatggggtc atctaccgca 421 gtggagagaa atttcagcca agctgcaaat tccagtgcac ctgcagagat gggcagattg 481 gctgtgtgcc ccgctgtcag ctggatgtgc tactgcctga gcctaactgc ccagctccaa 541 gaaaagttga ggtgcctgga gagtgctgtg aaaagtggat ctgtggccca gatgaggagg 601 attcactggg aggccttacc cttgcagctt acaggccaga agccacccta ggagtagaag 661 tctctgactc aagtgtcaac tgcattgaac agaccacaga gtggacagca tgctccaaga 721 gctgtggtat ggggttctcc acccgggtca ccaataggaa ccgtcaatgt gagatgctga 781 aacagactcg gctctgcatg gtgcggccct gtgaacaaga gccagagcag ccaacagata 841 agaaaggaaa aaagtgtctc cgcaccaaga agtcactcaa agccatccac ctgcagttca 901 agaactgcac cagcctgcac acctacaagc ccaggttctg tggggtctgc agtgatggcc 961 gctgctgcac tccccacaat accaaaacca tccaggcaga gtttcagtgc tccccagggc 1021 aaatagtcaa gaagccagtg atggtcattg ggacctgcac ctgtcacacc aactgtccta 1081 agaacaatga ggccttcctc caggagctgg agctgaagac taccagaggg aaaatgtacc 1141 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1201 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1261 tta