Construct: ORF ccsbBroad304_06656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009532.1_s304c1, BRDN0000406003
- Derived from:
- ccsbBroadEn_06656
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPPA (4878)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06656
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4878 | NPPA | natriuretic peptide A | NM_006172.4 | 98.6% | 98.6% | 453_454insCGAAGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ctccttctcc accaccaccg tgagcttcct ccttttactg gcattccagc 121 tcctaggtca gaccagagct aatcccatgt acaatgccgt gtccaacgca gacctgatgg 181 atttcaagaa tttgctggac catttggaag aaaagatgcc tttagaagat gaggtcgtgc 241 ccccacaagt gctcagtgag ccgaatgaag aagcgggggc tgctctcagc cccctccctg 301 aggtgcctcc ctggaccggg gaagtcagcc cagcccagag agatggaggt gccctcgggc 361 ggggcccctg ggactcctct gatcgatctg ccctcctaaa aagcaagctg agggcgctgc 421 tcactgcccc tcggagcctg cggagatcca gctgcttcgg gggcaggatg gacaggattg 481 gagcccagag cggactgggc tgtaacagct tccggtaccg aagataccca actttcttgt 541 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 601 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a