Construct: ORF ccsbBroad304_06696

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014634.1_s304c1, BRDN0000401500
Derived from:
ccsbBroadEn_06696
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCSK1 (5122)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06696
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5122 PCSK1 proprotein convertase subti... NM_000439.5 99.9% 99.7% 1993C>G;2069G>C
2 human 5122 PCSK1 proprotein convertase subti... NM_001177875.1 92.4% 91.4% (many diffs)
3 mouse 18548 Pcsk1 proprotein convertase subti... NM_013628.2 88.4% 92.4% (many diffs)
4 mouse 18548 Pcsk1 proprotein convertase subti... XM_006517153.2 88.4% 92.4% (many diffs)
5 mouse 18548 Pcsk1 proprotein convertase subti... XM_006517154.3 88.4% 92.4% (many diffs)
6 mouse 18548 Pcsk1 proprotein convertase subti... XM_006517155.2 88.4% 92.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2328
ORF length:
2259
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcgaaga gcctggagtc tgcagtgcac tgctttcgtc ctcttttgcg
121cttggtgtgc actgaacagt gcaaaagcga aaaggcaatt tgtcaatgaa tgggcagcgg
181agatccccgg gggcccggaa gcagcctcgg ccatcgccga ggagctgggc tatgaccttt
241tgggtcagat tggttcactt gaaaatcact acttattcaa acataaaaac caccccagaa
301ggtctcgaag gagtgccttt catatcacta agagattatc tgatgatgat cgtgtgatat
361gggctgaaca acagtatgaa aaagaaagaa gtaaacgttc agctctaagg gactcagcac
421taaatctctt caatgatccc atgtggaatc agcaatggta cttgcaagat accaggatga
481cggcagccct gcccaagctg gaccttcatg tgatacctgt ttggcaaaaa ggcattacgg
541gcaaaggagt tgttatcacc gtactggatg atggtttgga gtggaatcac acggacattt
601atgccaacta tgatccagag gctagctatg attttaatga taatgaccat gatccatttc
661cccgatatga tcccacaaac gagaacaaac acgggaccag atgtgcagga gaaattgcca
721tgcaagcaaa taatcacaaa tgcggggttg gagttgcata caattccaaa gttggaggca
781taagaatgct ggatggcatt gtgacggatg ctattgaggc cagttcaatt ggattcaatc
841ctggacacgt ggatatttac agtgcaagct ggggccctaa tgatgatggg aaaactgtgg
901aggggcctgg ccggctagcc cagaaggctt ttgaatatgg tgtcaaacag gggagacagg
961ggaaggggtc catcttcgtc tgggcttcgg gaaacggggg gcgtcaggga gataattgtg
1021actgtgatgg ctacacagac agcatctaca ccatctccat cagcagtgcc tcccagcaag
1081gcctatcccc ctggtacgct gagaagtgct cctccacact ggccacctct tacagcagcg
1141gagattacac cgaccagaga atcacgagcg ctgacctgca caatgactgc acggagacgc
1201acacaggcac ctcggcctct gcacctctgg ctgctggcat cttcgctctg gccctggaag
1261caaacccaaa tctcacctgg cgagatatgc agcacctggt tgtctggacc tctgagtatg
1321acccgctggc caataaccct ggatggaaaa agaatggagc aggcttgatg gtgaatagtc
1381gatttggatt tggcttgcta aatgccaaag ctctggtgga tttagctgac cccaggacct
1441ggaggagcgt gcctgagaag aaagagtgtg ttgtaaagga caatgacttt gagcccagag
1501ccctgaaagc taatggagaa gttatcattg aaattccaac aagagcttgt gaaggacaag
1561aaaatgctat caagtccctg gagcatgtac aatttgaagc aacaattgaa tattcccgaa
1621gaggagacct tcatgtcaca cttacttctg ctgctggaac tagcactgtg ctcttggctg
1681aaagagaacg ggatacatct cctaatggct ttaagaattg ggacttcatg tctgttcaca
1741catggggaga gaaccctata ggtacttgga ctttgagaat tacagacatg tctggaagaa
1801ttcaaaatga aggaagaatt gtgaactgga agctgatttt gcacgggacc tcttctcagc
1861cagagcatat gaagcagcct cgtgtgtaca cgtcctacaa cactgttcag aatgacagaa
1921gaggggtgga gaagatggtg gatccagggg aggagcagcc cacacaagag aaccctaagg
1981agaacaccct ggtgtccaaa agccccagca gcagcagcgt agggggccgg agggatgagt
2041tggaggaggg agccccttcc gaggccatgc tgcgactcct gcaaagtgct ttcagtaaaa
2101actcaccgcc aaagcaatca ccaaagaagt ccccaactgc aaagctcaac atcccttatg
2161aaaacttcta cgaagccctg gaaaagctga acaaaccttc ccagcttaaa gactctgaag
2221acagtctgta taatgactat gttgatgttt tttataacac taaaccttac aagcacagag
2281acgaccggct gcttcaagct ctggtggaca ttctgaatga ggaaaatttg ccaactttct
2341tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2401aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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