Construct: ORF ccsbBroad304_06711

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001126.1_s304c1, BRDN0000462320
Derived from:
ccsbBroadEn_06711
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFAS (5198)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06711
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5198 PFAS phosphoribosylformylglycina... NM_012393.3 99.8% 99.7% (many diffs)
2 human 5198 PFAS phosphoribosylformylglycina... XM_024450804.1 99.8% 99.7% (many diffs)
3 human 5198 PFAS phosphoribosylformylglycina... XM_017024760.1 80.9% 80.7% (many diffs)
4 human 5198 PFAS phosphoribosylformylglycina... XR_934047.2 73.1% (many diffs)
5 human 5198 PFAS phosphoribosylformylglycina... XM_006721546.3 70.6% 70.2% (many diffs)
6 mouse 237823 Pfas phosphoribosylformylglycina... NM_001159519.1 84.7% 88.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4083
ORF length:
4014
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtccccagtc cttcacttct atgttcgtcc ctctggccat gagggggcag
121cctctggaca cactcggagg aaactgcaag ggaaactgcc agagctgcag ggcgtcgaga
181ctgaactgtg ctacaacgtg aactggacag ctgaggccct ccccagtgct gaggagacaa
241agaagctgat gtggctgttt ggttgcccct tactgctgga tgatgttgct cgggagtcct
301ggctccttcc tggctccaat gacctgctgc tggaggtcgg gcccaggctg aacttctcca
361ccccaacatc caccaacatc gtgtcagtgt gccgcgccac tgggctgggg cctgtggatc
421gtgtggagac cacccggcgc taccggctct cgtttgccca ccccccgtca gctgaggtgg
481aagccattgc tctggctacc ctgcacgacc ggatgacaga gcagcacttc ccccatccca
541tccagagttt ctcccctgag agcatgccgg aacccctcaa tggccctatc aatatactgg
601gtgagggccg gcttgcgctg gagaaggcca accaggagct tggtctggct ttagactctt
661gggacctaga cttctacacc aagcgcttcc aggagctaca gcggaacccg agcactgtgg
721aggcctttga cttggcgcag tccaatagcg agcacagccg acactggttc ttcaagggcc
781agctccacgt ggatgggcag aagctggtgc actcactgtt tgagtccatc atgagcaccc
841aggaatcctc gaaccccaac aacgtcctca aattctgtga taacagcagt gcaatccagg
901gaaaggaagt ccgattccta cggcctgagg accccacacg gccaagccgc ttccagcaac
961agcaagggct gagacatgtt gtcttcacag cagagactca caactttccc acaggagtat
1021gcccctttag tggtgcaacc actggcacag ggggccggat tcgagatgtc cagtgcacag
1081gccgcggggc ccacgtggtg gctggcactg ccggctattg ctttggaaat ctgcatattc
1141caggttacaa tctgccctgg gaggatctaa gcttccagta tcctgggaat tttgcccggc
1201ccctggaggt tgccattgaa gccagtaatg gagcttctga ctatggcaac aagtttgggg
1261aaccagtgct ggctggcttc gcccgctcct tgggcctcca gctcccagac ggccagcggc
1321gtgagtggat caagcccatc atgtttagtg ggggcattgg gtccatggaa gctgaccaca
1381taagcaagga ggccccagag ccaggcatgg aagttgtaaa ggttggaggt cccgtctaca
1441ggattggagt tggaggtgga gctgcttcat ctgtgcaggt gcagggagat aacaccagtg
1501acctggactt tggggctgtg cagcgaggag acccggagat ggaacagaag atgaaccgtg
1561tgatcagggc ttgtgtggag gcccccaagg gaaaccccat ctgcagcctt catgatcagg
1621gcgctggtgg caatggcaat gtcctaaaag agctgagtga cccagctgga gccatcattt
1681acaccagccg cttccagctt ggggacccaa ccctgaatgc cctggaaatc tggggggctg
1741agtaccagga atcaaatgct cttctgctga ggtcccccaa ccgggacttc ctgactcatg
1801tcagtgcccg tgaacgttgc ccggcttgct tcgtgggcac catcactgga gaccggagaa
1861tagtgctggt ggacgatcgg gagtgtcctg tcagaagaaa tggccagggg gatgcccccc
1921cgacaccccc gccaacccct gtggacctgg agctcgaatg ggtgctgggc aagatgcctc
1981ggaaggagtt cttcctgcag aggaagcccc ccatgctgca gcctctggcc ttgcccccag
2041ggctgagcgt gcaccaggct ctggagaggg ttctgaggct gcccgccgtg gccagcaagc
2101gctacctcac caataaggtg gaccgctccg tgggaggcct ggtggcccag cagcagtgcg
2161tggggcccct gcaaactcct ctggcagatg tagcggttgt ggcactgagc catgaggagc
2221tcataggggc tgccacagcc ttgggagaac agccagtcaa gagcctgctg gacccaaaag
2281tcgccgcccg gctggccgtg gccgaagccc tcaccaacct ggtgtttgct ctggtcactg
2341acctccggga tgtgaagtgt agcgggaact ggatgtgggc agccaagctc ccaggggagg
2401gcgcagcttt ggcggatgcc tgtgaggcta tggtggcagt gatggcagcc ctgggtgtgg
2461cagtggatgg tggcaaggac tccctcagca tggctgctcg ggttggcact gagaccgtgc
2521gggctcctgg gtcactggtc atctcagcct atgccgtctg cccagacatc acagccactg
2581tgaccccaga cctcaagcat cctgaaggga gaggccatct gctctatgtg gctctgagcc
2641ctgggcagca ccggctcggg ggcacagctc tggcccagtg cttctcccag cttggggaac
2701accctccaga cctggacctt cctgagaact tggtgcgggc cttcagcatc actcaggggc
2761tgctgaaaga ccgcctcctc tgctcaggcc acgatgtcag tgacggaggc ctcgtcacat
2821gcctgctgga gatggccttt gctggaaatt gcgggctaca ggtggatgtg cctgtcccca
2881gggttgatgt cctgtctgtg ctgttcgctg aggagccagg cctcgtgctg gaggtgcagg
2941agccagacct ggcccaggtg ctgaagcgtt accgggatgc tggcctccat tgcctggagc
3001tgggccacac aggcgaggcc gggccccacg ccatggtccg ggtgtcagtg aacggggctg
3061tggttctgga ggagcctgtt ggggagctgc gagccctctg ggaggagacg agtttccagc
3121tggaccggct acaggcagag cctcgctgtg tggcagagga ggaacggggc ctgagggagc
3181ggatggggcc cagctattgc ctgcccccca cctttcccaa agcctccgtg ccccgtgagc
3241ctggtggtcc cagcccccga gtcgccatct tgcgagagga gggcagtaat ggagaccggg
3301agatggccga tgccttccac ttagctgggt ttgaggtatg ggacgtgacc atgcaggacc
3361tctgctctgg ggcaattggg ctggacactt tccgtggcgt ggccttcgtg ggcggcttca
3421gctatgcaga tgtcctgggc tctgccaaag ggtgggcagc tgctgtgacc tttcatccca
3481gggctggggc tgagctgagg cgcttccgga agcggccaga caccttcagc ctgggcgtgt
3541gtaatggctg tcaactgctg gctctgctcg gctgggtggg aggcgacccc aatgaggatg
3601ctgcagagat gggccctgac tcccagccag cccggccagg ccttctgcta cgccacaacc
3661tgtctgggcg ctacgagtct cgctgggcca gcgtgcgtgt ggggcctggg ccagccctga
3721tgctgcgagg gatggagggc gccgtgctgc ccgtgtggag tgcgcacggg gaaggttacg
3781tagcattttc ttctccggaa ctccaagctc agattgaggc caggggcttg gctccactgc
3841actgggctga tgatgacggg aaccccacag agcagtaccc tctgaatccc aatgggtccc
3901cagggggcgt ggctggcatc tgctcctgtg atggccgcca cctggctgtc atgcctcacc
3961ctgagcgggc cgttaggcct tggcagtggg catggcgacc ccctccattt gatactctga
4021ccacctcccc ctggctccag ctctctatca atgcccgaaa ctggaccctg gaagggagct
4081gcttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
4141tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
4201tctggatta

Download FASTA (ORF) (Full)

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