Construct: ORF ccsbBroad304_06742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007924.1_s304c1, BRDN0000400351
- Derived from:
- ccsbBroadEn_06742
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PML (5371)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06742
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5371 | PML | promyelocytic leukemia | NM_033250.3 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 5371 | PML | promyelocytic leukemia | NM_033239.3 | 94% | 93.8% | (many diffs) |
| 3 | human | 5371 | PML | promyelocytic leukemia | NM_033249.2 | 72.1% | 67.4% | (many diffs) |
| 4 | human | 5371 | PML | promyelocytic leukemia | NM_033238.3 | 71.6% | 62.1% | (many diffs) |
| 5 | human | 5371 | PML | promyelocytic leukemia | NM_002675.3 | 68% | 63.5% | (many diffs) |
| 6 | human | 5371 | PML | promyelocytic leukemia | NM_033240.3 | 66.5% | 63.8% | (many diffs) |
| 7 | human | 5371 | PML | promyelocytic leukemia | NM_033244.4 | 61.1% | 60.8% | (many diffs) |
| 8 | human | 5371 | PML | promyelocytic leukemia | NM_033247.3 | 54.7% | 53.3% | (many diffs) |
| 9 | human | 5371 | PML | promyelocytic leukemia | NM_033246.2 | 53.6% | 53.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2409
- ORF length:
- 2343
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gcctgcaccc gcccgatctc cgaggcccca gcaggacccc gcccggcccc 121 aggagcccac catgcctccc cccgagaccc cctctgaagg ccgccagccc agccccagcc 181 ccagccctac agagcgagcc cccgcttcgg aggaggagtt ccagtttctg cgctgccagc 241 aatgccaggc ggaagccaag tgcccgaagc tgctgccttg tctgcacacg ctgtgctcag 301 gatgcctgga ggcgtcgggc atgcagtgcc ccatctgcca ggcgccctgg cccctaggtg 361 cagacacacc cgccctggat aacgtctttt tcgagagtct gcagcggcgc ctgtcggtgt 421 accggcagat tgtggatgcg caggctgtgt gcacccgctg caaagagtcg gccgacttct 481 ggtgctttga gtgcgagcag ctcctctgcg ccaagtgctt cgaggcacac cagtggttcc 541 tcaagcacga ggcccggccc ctagcagagc tgcgcaacca gtcggtgcgt gagttcctgg 601 acggcacccg caagaccaac aacatcttct gctccaaccc caaccaccgc acccctacgc 661 tgaccagcat ctactgccga ggatgttcca agccgctgtg ctgctcgtgc gcgctccttg 721 acagcagcca cagtgatctc aagtgcgaca tcagcgcaga gatccagcag cgacaggagg 781 agctggacgc catgacgcag gcgctgcagg agcaggatag tgcctttggc gcggttcacg 841 cgcagatgca cgcggctgtc ggccagctgg gccgcgcgcg tgccgagacc gaggagctga 901 tccgcgagcg cgtgcgccag gtggtagctc acgtgcgggc tcaggagcgc gagctgctgg 961 aggctgtgga cgcgcggtac cagcgcgact acgaggagat ggccagtcgg ctgggccgcc 1021 tggatgctgt gctgcagcgc atccgcacgg gcagcgcgct ggtgcagagg atgaagtgct 1081 acgcctcgga ccaggaggtg ctggacatgc acggtttcct gcgccaggcg ctctgccgcc 1141 tgcgccagga ggagccccag agcctgcaag ctgccgtgcg caccgatggc ttcgacgagt 1201 tcaaggtgcg cctgcaggac ctcagctctt gcatcaccca ggggaaagat gcagctgtat 1261 ccaagaaagc cagcccagag gctgccagca ctcccaggga ccctattgac gttgacctgg 1321 atgtctccaa tacaacgaca gcccagaaga ggaagtgcag ccagacccag tgccccagga 1381 aggtcatcaa gatggagtct gaggagggga aggaggcaag gttggctcgg agctccccgg 1441 agcagcccag gcccagcacc tccaaggcag tctcaccacc ccacctggat ggaccgccta 1501 gccccaggag ccccgtcata ggaagtgagg tcttcctgcc caacagcaac cacgtggcca 1561 gtggcgccgg ggaggcagag gaacgcgttg tggtgatcag cagctcggaa gactcagatg 1621 ccgaaaactc gtgcatggag cccatggaga ccgccgagcc acagtcctcg ccagcccact 1681 cctcgccagc ccactcctcg ccagcccact cctcgccagt ccagtctctg ctgagagcac 1741 aaggagcctc cagcctgccc tgtggcacat accacccccc agcttggcct ccccaccagc 1801 ccgctgagca ggctgccacc cccgatgctg agcctcacag cgagcctcct gatcaccagg 1861 agcgccctgc cgtccaccgt gggatccgct acctgttgta cagagcacag agagccatcc 1921 gccttcgcca tgccctccgc ttgcaccctc aattgcatcg ggcccctatt cggacttggt 1981 ctccccatgt ggtccaagcc agcactcctg ccatcacagg gcccctcaac catcctgcca 2041 atgcccagga acatcctgcc cagctgcaaa ggggcatcag cccaccccac cggatacgag 2101 gggctgtgcg atcccgcagc cgctccctcc ggggctcctc ccatttatcc cagtggctca 2161 acaacttttt tgccctcccc ttctcctcca tggcttccca gcttgacatg tcttccgtgg 2221 tgggggcagg ggaaggcaga gcccagactc ttggagcagt tgttccccct ggggactctg 2281 tcagaggctc catggaggcc tctcaagtcc aagtgcctct ggaagcctct ccaattacat 2341 tcccaccacc ctgtgcccca gaaaggcccc ccatcagccc agtcccaggc gcccgtcaag 2401 caggcctctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2461 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2521 tcaacctctg gatta