Construct: ORF ccsbBroad304_06806

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012419.1_s304c1, BRDN0000400260
Derived from:
ccsbBroadEn_06806
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMD3 (5709)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06806
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5709 PSMD3 proteasome 26S subunit, non... NM_002809.4 99.8% 99.8% 141G>A;911A>T
2 mouse 22123 Psmd3 proteasome (prosome, macrop... NM_009439.1 89.3% 97.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1668
ORF length:
1602
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gcaggagggc tcggcgcggc gccgcggcgc ggacaaggcg aaaccgccgc
121ccggcggagg agaacaagaa cccccaccgc cgccggcccc ccaggatgtg gagatgaaag
181aggaggcagc gacgggtggc gggtcaacgg gggaggcaga cggcaagacg gcggcggcag
241cggctgagca ctcccagcga gagctggaca cagtcacctt ggaggacatc aaggagcacg
301tgaaacagct agagaaagcg gtttcaggca aggagccgag attcgtgctg cgggccctgc
361ggatgctgcc ttccacatca cgccgcctca accactatgt tctgtataag gctgtgcagg
421gcttcttcac ttcaaataat gccactcgag actttttgct ccccttcctg gaagagccca
481tggacacaga ggctgattta cagttccgtc cccgcacggg aaaagctgcg tcgacacccc
541tcctgcctga agtggaagcc tatctccaac tcctcgtggt catcttcatg atgaacagca
601agcgctacaa agaggcacag aagatctctg atgatctgat gcagaagatc agtactcaga
661accgccgggc cctagacctt gtagccgcaa agtgttacta ttatcacgcc cgggtctatg
721agttcctgga caagctggat gtggtgcgca gcttcttgca tgctcggctc cggacagcta
781cgcttcggca tgacgcagac gggcaggcca ccctgttgaa cctcctgctg cggaattacc
841tacactacag cttgtacgac caggctgaga agctggtgtc caagtctgtg ttcccagagc
901aggccaacaa caatgagtgg gccaggtacc tctactacac agggcgaatc aaagccatcc
961agctggagta ctcagtggcc cggagaacga tgaccaacgc ccttcgcaag gcccctcagc
1021acacagctgt cggcttcaaa cagacggtgc acaagcttct catcgtggtg gagctgttgc
1081tgggggagat ccctgaccgg ctgcagttcc gccagccctc cctcaagcgc tcactcatgc
1141cctatttcct tctgactcaa gctgtcagga caggaaacct agccaagttc aaccaggtcc
1201tggatcagtt tggggagaag tttcaagcag atgggaccta caccctaatt atccggctgc
1261ggcacaacgt gattaagaca ggtgtacgca tgatcagcct ctcctattcc cgaatctcct
1321tggctgacat cgcccagaag ctgcagttgg atagccccga agatgcagag ttcattgttg
1381ccaaggccat ccgggatggt gtcattgagg ccagcatcaa ccacgagaag ggctatgtcc
1441aatccaagga gatgattgac atctattcca cccgagagcc ccagctagcc ttccaccagc
1501gcatctcctt ctgcctagat atccacaaca tgtctgtcaa ggccatgagg tttcctccca
1561aatcgtacaa caaggacttg gagtctgcag aggaacggcg tgagcgagaa cagcaggact
1621tggagtttgc caaggagatg gcagaagatg atgatgacag cttcccttgc cccactttct
1681tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1741aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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