Construct: ORF ccsbBroad304_06809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007138.1_s304c1, BRDN0000407553
- Derived from:
- ccsbBroadEn_06809
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTBP1 (5725)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06809
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_002819.5 | 99.9% | 99.8% | 1664C>A |
| 2 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XM_005259597.2 | 99.4% | 98.9% | (many diffs) |
| 3 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_031990.4 | 98.6% | 98.5% | 891_892ins21;1643C>A |
| 4 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_031991.4 | 95.2% | 95.1% | 891_892ins78;1586C>A |
| 5 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XM_005259598.2 | 94.8% | 94.2% | (many diffs) |
| 6 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XR_244034.2 | 43.3% | (many diffs) | |
| 7 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XR_244035.2 | 41.2% | (many diffs) | |
| 8 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_001077363.2 | 86.8% | 96.7% | (many diffs) |
| 9 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_008956.3 | 82.8% | 92.2% | (many diffs) |
| 10 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_001283013.1 | 77% | 85.6% | (many diffs) |
| 11 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | XR_380379.1 | 66.5% | (many diffs) | |
| 12 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | XR_001779485.1 | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1737
- ORF length:
- 1671
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cggcattgtc ccagatatag ccgttggtac aaagcgggga tctgacgagc 121 ttttctctac ttgtgtcact aacggaccgt ttatcatgag cagcaactcg gcttctgcag 181 caaacggaaa tgacagcaag aagttcaaag gtgacagccg aagtgcaggc gtcccctcta 241 gagtgatcca catccggaag ctccccatcg acgtcacgga gggggaagtc atctccctgg 301 ggctgccctt tgggaaggtc accaacctcc tgatgctgaa ggggaaaaac caggccttca 361 tcgagatgaa cacggaggag gctgccaaca ccatggtgaa ctactacacc tcggtgaccc 421 ctgtgctgcg cggccagccc atctacatcc agttctccaa ccacaaggag ctgaagaccg 481 acagctctcc caaccaggcg cgggcccagg cggccctgca ggcggtgaac tcggtccagt 541 cggggaacct ggccttggct gcctcggcgg cggccgtgga cgcagggatg gcgatggccg 601 ggcagagccc cgtgctcagg atcatcgtgg agaacctctt ctaccctgtg accctggatg 661 tgctgcacca gattttctcc aagttcggca cagtgttgaa gatcatcacc ttcaccaaga 721 acaaccagtt ccaggccctg ctgcagtatg cggaccccgt gagcgcccag cacgccaagc 781 tgtcgctgga cgggcagaac atctacaacg cctgctgcac gctgcgcatc gacttttcca 841 agctcaccag cctcaacgtc aagtacaaca atgacaagag ccgtgactac acacgcccag 901 acctgccttc cggggacagc cagccctcgc tggaccagac catggccgcg gccttcggtg 961 cacctggtat aatctcagcc tctccgtatg caggagctgg tttccctccc acctttgcca 1021 ttcctcaagc tgcaggcctt tccgttccga acgtccacgg cgccctggcc cccctggcca 1081 tcccctcggc ggcggcggca gctgcggcgg caggtcggat cgccatcccg ggcctggcgg 1141 gggcaggaaa ttctgtattg ctggtcagca acctcaaccc agagagagtc acaccccaaa 1201 gcctctttat tcttttcggc gtctacggtg acgtgcagcg cgtgaagatc ctgttcaata 1261 agaaggagaa cgccctagtg cagatggcgg acggcaacca ggcccagctg gccatgagcc 1321 acctgaacgg gcacaagctg cacgggaagc ccatccgcat cacgctctcg aagcaccaga 1381 acgtgcagct gccccgcgag ggccaggagg accagggcct gaccaaggac tacggcaact 1441 cacccctgca ccgcttcaag aagccgggct ccaagaactt ccagaacata ttcccgccct 1501 cggccacgct gcacctctcc aacatcccgc cctcagtctc cgaggaggat ctcaaggtcc 1561 tgttttccag caatgggggc gtcgtcaaag gattcaagtt cttccagaag gaccgcaaga 1621 tggcactgat ccagatgggc tccgtggagg aggcggtcca ggccctcatt gacctgcaca 1681 accacgacct cggggagaac caccacctgc gggtctcctt ctccaagtac accatctatc 1741 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1801 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1861 tta