Construct: ORF ccsbBroad304_06942

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016786.1_s304c1, BRDN0000406980
Derived from:
ccsbBroadEn_06942
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCG5 (6447)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06942
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6447 SCG5 secretogranin V NM_001144757.2 99.8% 99.5% 452T>C
2 human 6447 SCG5 secretogranin V NM_003020.4 99.3% 99% 373_374insCAG;449T>C
3 human 114118903 ARHGAP11A-SCG5 ARHGAP11A-SCG5 readthrough NM_001368319.1 33.6% 33.5% 1_1242del;1615_1616insCAG;1691T>C
4 mouse 20394 Scg5 secretogranin V NM_009162.3 87.5% 91.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
702
ORF length:
636
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ctccaggatg gtctctacca tgctatctgg cctactgttt tggctggcat
121ctggatggac tccagcattt gcttacagcc cccggacccc tgaccgggtc tcagaagcag
181atatccagag gctgcttcat ggtgttatgg agcaattggg cattgccagg ccccgagtgg
241aatatccagc tcaccaggcc atgaatcttg tgggccccca gagcattgaa ggtggagctc
301atgaaggact tcagcatttg ggtccttttg gcaacatccc caacatcgtg gcagagttga
361ctggagacaa cattcctaag gactttagtg aggatcaggg gtacccagac cctccaaatc
421cctgtcctgt tggaaaaaca gcagatgatg gatgtctaga aaacacccct gacactgcag
481agttcagtcg agagttccag ttgcaccagc atctctctga tccggaacat gactatccag
541gcttgggcaa gtggaacaag aaactccttt acgagaagat gaagggagga gagagacgaa
601agcggaggag tgtcaatcca tatctacaag gacagagact ggataatgtt gttgcaaaga
661agtctgtccc ccatttttca gatgaggata aggatccaga gtacccaact ttcttgtaca
721aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
781ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)