Construct: ORF ccsbBroad304_06990

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018380.1_s304c1, BRDN0000410561
Derived from:
ccsbBroadEn_06990
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPOCK1 (6695)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06990
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6695 SPOCK1 SPARC (osteonectin), cwcv a... NM_004598.4 99.8% 99.5% 229C>A;442T>C
2 mouse 20745 Spock1 sparc/osteonectin, cwcv and... NM_001166463.1 89.5% 94.7% (many diffs)
3 mouse 20745 Spock1 sparc/osteonectin, cwcv and... NM_009262.3 88.9% 94.1% (many diffs)
4 mouse 20745 Spock1 sparc/osteonectin, cwcv and... NM_001166466.1 88.7% 93.8% (many diffs)
5 mouse 20745 Spock1 sparc/osteonectin, cwcv and... NM_001166465.1 72.5% 67.4% (many diffs)
6 mouse 20745 Spock1 sparc/osteonectin, cwcv and... NM_001166464.1 72.1% 67% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1383
ORF length:
1317
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc ggcgatcgcg gtgttggcgg cggccgccgc ggcgtggtgc ttcctccaag
121tcgagagccg gcacctggac gcgctcgccg gaggcgcggg ccccaaccac ggcaatttcc
181tagacaatga ccagtggctg agcaccgtct cccagtacga ccgggacaag tactggaacc
241gctttcgaga cgatgattat ttcagaaact ggaatcccaa caagcccttt gacaaagccc
301tggacccatc caaggacccc tgcctgaagg taaaatgcag ccctcacaaa gtgtgtgtga
361cccaggacta ccagaccgcc ctgtgtgtca gccgcaagca cctgctcccc aggcaaaaga
421aggggaacgt ggcccagaaa cactgggttg gaccttcgaa tttggtcaag tgcaagccct
481gtcccgtggc acagtcagcc atggtccgcg gctcagatgg ccactcctac acatccaagt
541gcaaattgga gttccatgct tgttctactg gcaaaagcct cgccaccctc tgtgatgggc
601cctgtccctg tctcccagag cctgagccac caaagcacaa ggcagaaagg agtgcctgca
661cagacaagga gttgcggaac cttgcctccc ggctgaagga ttggtttgga gctctccacg
721aggatgcgaa cagagtcatc aagcccacca gctccaacac agcccaaggc aggtttgaca
781ctagcatcct gcccatctgc aaggactccc tgggctggat gttcaacaag ttggacatga
841actatgacct cctgcttgac ccttcagaga tcaatgccat ctacctggat aagtacgagc
901cctgtatcaa gcctcttttc aactcgtgtg actccttcaa ggatggcaag ctttctaaca
961atgagtggtg ctactgcttc cagaagcctg gaggtctccc ttgccagaat gaaatgaaca
1021gaattcagaa gctgagtaag gggaaaagcc tgttgggggc cttcatacct cggtgtaatg
1081aggagggcta ttacaaagcc acacagtgcc acggcagcac ggggcagtgc tggtgtgtgg
1141acaaatatgg gaatgagttg gctggctcca ggaaacaggg tgctgtgagc tgtgaagagg
1201agcaggaaac ctcaggggat tttggcagtg gtgggtccgt ggtcctgctg gatgacctag
1261aatatgaacg ggagctggga ccaaaggaca aagaggggaa gctgagggtg cacacccgag
1321ccgtgacaga ggatgatgag gatgaggatg atgacaaaga ggatgaggtc gggtacatat
1381ggtacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1441tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1501tctggatta

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