Transcript: Mouse NM_001166466.1

Mus musculus sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (Spock1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Spock1 (20745)
Length:
4671
CDS:
142..1467

Additional Resources:

NCBI RefSeq record:
NM_001166466.1
NBCI Gene record:
Spock1 (20745)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001166466.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000079971 CCACTCAGAGATCAATGCCAT pLKO.1 942 CDS 100% 2.640 3.696 N Spock1 n/a
2 TRCN0000079968 GCCATCTACCTAGACAAATAT pLKO.1 958 CDS 100% 15.000 10.500 N Spock1 n/a
3 TRCN0000079969 CCCTTGCCAGAATGAAATGAA pLKO.1 1080 CDS 100% 5.625 3.938 N Spock1 n/a
4 TRCN0000079970 CAACGCCAATTTCCTAGACAA pLKO.1 243 CDS 100% 4.950 3.465 N Spock1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166466.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06990 pDONR223 100% 88.7% 93.8% None (many diffs) n/a
2 ccsbBroad304_06990 pLX_304 0% 88.7% 93.8% V5 (many diffs) n/a
3 TRCN0000468464 CACACTGGGAAGAATCTGCAATTA pLX_317 27.5% 88.7% 93.8% V5 (many diffs) n/a
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