Construct: ORF ccsbBroad304_07018

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005007.1_s304c1, BRDN0000404941
Derived from:
ccsbBroadEn_07018
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SUPT5H (6829)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07018
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_001111020.3 99.9% 100% 162C>T;177G>A
2 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_001130824.2 99.9% 100% 162C>T;177G>A
3 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_001319990.2 99.9% 100% 162C>T;177G>A
4 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_003169.3 99.9% 100% 162C>T;177G>A
5 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_001130825.1 99.5% 99.6% 162C>T;177G>A;304_305insAAGAAGAGATTG
6 human 6829 SUPT5H SPT5 homolog, DSIF elongati... NM_001319991.2 99.5% 99.6% 162C>T;177G>A;304_305insAAGAAGAGATTG
7 human 6829 SUPT5H SPT5 homolog, DSIF elongati... XM_017027174.2 58.6% 58.6% 0_1ins1350
8 mouse 20924 Supt5 suppressor of Ty 5, DSIF el... NM_013676.1 88.5% 97.3% (many diffs)
9 mouse 20924 Supt5 suppressor of Ty 5, DSIF el... XM_006539702.2 88.1% 96.9% (many diffs)
10 mouse 20924 Supt5 suppressor of Ty 5, DSIF el... XM_006539703.2 84.3% 92.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3327
ORF length:
3261
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ggacagcgag gacagcaact tttccgagga ggaggacagc gagcgcagca
121gtgacggcga ggaggccgag gtagacgaag agcggcggag tgcagcgggc agtgagaaag
181aagaagagcc tgaggacgaa gaggaggagg aagaggagga ggaatatgat gaggaagagg
241aagaagaaga tgatgaccga ccccccaaga aaccccgcca tggaggcttc attctggacg
301aggctgatgt tgacgatgag tatgaggacg aggaccagtg ggaggatgga gcagaggaca
361ttctagagaa agaagagatt gaagcctcca atatcgataa tgttgtcctg gatgaagatc
421gttctggggc tcgccgcctg caaaacctct ggagggacca gcgagaagaa gaactgggcg
481agtattacat gaagaaatac gccaagtcat ctgtgggaga gacggtgtat ggaggatctg
541atgagctctc agacgacatc acccagcagc agctgctccc aggagtcaag gatcccaatc
601tgtggactgt caaatgtaag attggggagg aacgggccac ggccatttcc ttgatgcgca
661agttcattgc ctaccagttc acagacacgc ccctgcagat caagtcagta gtggcaccag
721agcatgtgaa gggctacatc tacgtggagg cctacaagca gacccacgtg aagcaggcca
781ttgagggggt gggcaacctg cggcttggct actggaacca gcagatggtg cccatcaagg
841agatgacaga cgtgctcaaa gtggtgaagg aggtggccaa cctgaaacca aagtcctggg
901tccgcctcaa gcggggcatc tacaaggatg acattgctca ggtggactac gtggagccca
961gccagaacac catctccctg aagatgatcc cacgcatcga ctacgatcgc atcaaggccc
1021gcatgagctt gaaagactgg tttgccaaaa ggaagaagtt taagcggcct ccacagaggc
1081tgtttgatgc tgagaagatc aggtccctgg ggggtgatgt tgcctctgat ggtgacttcc
1141tcatctttga ggggaaccgt tacagccgga agggctttct gttcaagagc ttcgccatgt
1201ctgctgtgat cacggagggt gtgaagccaa cactctctga gctggaaaag tttgaggacc
1261agccagaggg cattgacctg gaggtggtga ctgagagcac agggaaggag cgggagcaca
1321acttccaacc tggggacaac gtggaggtct gtgagggtga gctcatcaac ctgcagggca
1381agatcctcag cgtggatggc aacaagatca ccatcatgcc caagcatgag gacctcaagg
1441acatgttgga gttcccagcc caggaactta gaaaatactt caagatgggg gaccacgtga
1501aggtgattgc tggccgattc gagggcgaca caggcctcat tgtgcgggtg gaggagaatt
1561tcgttatcct gttctctgac ctcaccatgc atgagctgaa ggtgctcccc cgggacctgc
1621agctctgctc agagacagca tcaggtgtgg atgttggggg ccagcatgaa tggggcgagc
1681tggtgcagct ggatccccag actgtgggtg tcatcgtgcg actagaacgg gagaccttcc
1741aggtgctgaa catgtacggg aaggtggtga ctgtcagaca tcaggctgtg acccggaaga
1801aggacaaccg ctttgctgtg gccttggact cagagcagaa caacatccat gtgaaagaca
1861tcgttaaggt cattgatggc ccccactcag gccgagaagg ggagattcgc catctcttcc
1921gaagcttcgc cttcctacat tgcaagaaac tggtggagaa cgggggcatg tttgtctgca
1981agacccgcca cctggtgctg gctgggggct caaagccccg tgatgtgacc aacttcaccg
2041tgggtggctt tgcgcctatg agtccccgga tcagcagccc catgcacccc agtgctggag
2101gtcagcgtgg cggctttggt agcccaggtg gcggcagtgg tggcatgagc aggggccggg
2161gccggaggga caacgaactc atcggccaga ccgtgcgcat ctcccagggg ccctacaaag
2221gctacatcgg tgtggtgaaa gatgccacag agtccacggc ccgtgtggag ctgcactcca
2281cctgccagac catctctgtg gaccgtcagc ggctcaccac ggtgggctca cggcgcccgg
2341gcggcatgac ctcgacctat gggaggacgc ccatgtatgg ctcccagacg cccatgtatg
2401gctctggctc ccgaacaccc atgtacggct cacagacacc cctccaggat ggtagccgca
2461ccccacacta cggctcacag acgcccctgc atgatggcag ccgcactcct gcccagagtg
2521gggcctggga ccccaacaac cccaacacgc cgtcacgggc tgaggaagaa tatgagtatg
2581ctttcgatga tgagcccacc ccgtccccgc aggcctatgg gggaaccccc aatccccaaa
2641cacctggcta cccagacccc tcgtccccac aggtcaaccc acaatacaac ccgcagacgc
2701cagggacgcc ggccatgtac aacacagacc agttctctcc ctatgctgcc ccctccccac
2761aaggttccta ccagcccagc cccagccccc agagctacca ccaggtggcg ccaagcccag
2821caggctacca gaatacccac tccccagcca gctaccaccc tacaccgtcg cccatggcct
2881atcaggctag ccccagcccg agccccgttg gctacagtcc tatgacacct ggagctccct
2941cccctggtgg ctacaaccca cacacgccag gctcaggcat cgagcagaac tccagcgact
3001gggtaaccac tgacattcag gtgaaggtgc gggacaccta cctggataca caggtggtgg
3061gacagacagg tgtcatccgc agtgtcacgg ggggcatgtg ctctgtgtac ctgaaggaca
3121gtgagaaggt tgtcagcatt tccagtgagc acctggagcc tatcaccccc accaagaaca
3181acaaggtgaa agtgatcctg ggcgaggatc gggaagccac gggcgtccta ctgagcattg
3241atggtgagga tggcattgtc cgtatggacc ttgatgagca gctcaagatc ctcaacctcc
3301gcttcctggg gaagctcctg gaagcctgcc caactttctt gtacaaagtg gttggtaagc
3361ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
3421ggcgcgttaa gtcgacaatc aacctctgga tta

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