Construct: ORF ccsbBroad304_07018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005007.1_s304c1, BRDN0000404941
- Derived from:
- ccsbBroadEn_07018
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUPT5H (6829)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07018
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_001111020.3 | 99.9% | 100% | 162C>T;177G>A |
| 2 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_001130824.2 | 99.9% | 100% | 162C>T;177G>A |
| 3 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_001319990.2 | 99.9% | 100% | 162C>T;177G>A |
| 4 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_003169.3 | 99.9% | 100% | 162C>T;177G>A |
| 5 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_001130825.1 | 99.5% | 99.6% | 162C>T;177G>A;304_305insAAGAAGAGATTG |
| 6 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | NM_001319991.2 | 99.5% | 99.6% | 162C>T;177G>A;304_305insAAGAAGAGATTG |
| 7 | human | 6829 | SUPT5H | SPT5 homolog, DSIF elongati... | XM_017027174.2 | 58.6% | 58.6% | 0_1ins1350 |
| 8 | mouse | 20924 | Supt5 | suppressor of Ty 5, DSIF el... | NM_013676.1 | 88.5% | 97.3% | (many diffs) |
| 9 | mouse | 20924 | Supt5 | suppressor of Ty 5, DSIF el... | XM_006539702.2 | 88.1% | 96.9% | (many diffs) |
| 10 | mouse | 20924 | Supt5 | suppressor of Ty 5, DSIF el... | XM_006539703.2 | 84.3% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3327
- ORF length:
- 3261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggacagcgag gacagcaact tttccgagga ggaggacagc gagcgcagca 121 gtgacggcga ggaggccgag gtagacgaag agcggcggag tgcagcgggc agtgagaaag 181 aagaagagcc tgaggacgaa gaggaggagg aagaggagga ggaatatgat gaggaagagg 241 aagaagaaga tgatgaccga ccccccaaga aaccccgcca tggaggcttc attctggacg 301 aggctgatgt tgacgatgag tatgaggacg aggaccagtg ggaggatgga gcagaggaca 361 ttctagagaa agaagagatt gaagcctcca atatcgataa tgttgtcctg gatgaagatc 421 gttctggggc tcgccgcctg caaaacctct ggagggacca gcgagaagaa gaactgggcg 481 agtattacat gaagaaatac gccaagtcat ctgtgggaga gacggtgtat ggaggatctg 541 atgagctctc agacgacatc acccagcagc agctgctccc aggagtcaag gatcccaatc 601 tgtggactgt caaatgtaag attggggagg aacgggccac ggccatttcc ttgatgcgca 661 agttcattgc ctaccagttc acagacacgc ccctgcagat caagtcagta gtggcaccag 721 agcatgtgaa gggctacatc tacgtggagg cctacaagca gacccacgtg aagcaggcca 781 ttgagggggt gggcaacctg cggcttggct actggaacca gcagatggtg cccatcaagg 841 agatgacaga cgtgctcaaa gtggtgaagg aggtggccaa cctgaaacca aagtcctggg 901 tccgcctcaa gcggggcatc tacaaggatg acattgctca ggtggactac gtggagccca 961 gccagaacac catctccctg aagatgatcc cacgcatcga ctacgatcgc atcaaggccc 1021 gcatgagctt gaaagactgg tttgccaaaa ggaagaagtt taagcggcct ccacagaggc 1081 tgtttgatgc tgagaagatc aggtccctgg ggggtgatgt tgcctctgat ggtgacttcc 1141 tcatctttga ggggaaccgt tacagccgga agggctttct gttcaagagc ttcgccatgt 1201 ctgctgtgat cacggagggt gtgaagccaa cactctctga gctggaaaag tttgaggacc 1261 agccagaggg cattgacctg gaggtggtga ctgagagcac agggaaggag cgggagcaca 1321 acttccaacc tggggacaac gtggaggtct gtgagggtga gctcatcaac ctgcagggca 1381 agatcctcag cgtggatggc aacaagatca ccatcatgcc caagcatgag gacctcaagg 1441 acatgttgga gttcccagcc caggaactta gaaaatactt caagatgggg gaccacgtga 1501 aggtgattgc tggccgattc gagggcgaca caggcctcat tgtgcgggtg gaggagaatt 1561 tcgttatcct gttctctgac ctcaccatgc atgagctgaa ggtgctcccc cgggacctgc 1621 agctctgctc agagacagca tcaggtgtgg atgttggggg ccagcatgaa tggggcgagc 1681 tggtgcagct ggatccccag actgtgggtg tcatcgtgcg actagaacgg gagaccttcc 1741 aggtgctgaa catgtacggg aaggtggtga ctgtcagaca tcaggctgtg acccggaaga 1801 aggacaaccg ctttgctgtg gccttggact cagagcagaa caacatccat gtgaaagaca 1861 tcgttaaggt cattgatggc ccccactcag gccgagaagg ggagattcgc catctcttcc 1921 gaagcttcgc cttcctacat tgcaagaaac tggtggagaa cgggggcatg tttgtctgca 1981 agacccgcca cctggtgctg gctgggggct caaagccccg tgatgtgacc aacttcaccg 2041 tgggtggctt tgcgcctatg agtccccgga tcagcagccc catgcacccc agtgctggag 2101 gtcagcgtgg cggctttggt agcccaggtg gcggcagtgg tggcatgagc aggggccggg 2161 gccggaggga caacgaactc atcggccaga ccgtgcgcat ctcccagggg ccctacaaag 2221 gctacatcgg tgtggtgaaa gatgccacag agtccacggc ccgtgtggag ctgcactcca 2281 cctgccagac catctctgtg gaccgtcagc ggctcaccac ggtgggctca cggcgcccgg 2341 gcggcatgac ctcgacctat gggaggacgc ccatgtatgg ctcccagacg cccatgtatg 2401 gctctggctc ccgaacaccc atgtacggct cacagacacc cctccaggat ggtagccgca 2461 ccccacacta cggctcacag acgcccctgc atgatggcag ccgcactcct gcccagagtg 2521 gggcctggga ccccaacaac cccaacacgc cgtcacgggc tgaggaagaa tatgagtatg 2581 ctttcgatga tgagcccacc ccgtccccgc aggcctatgg gggaaccccc aatccccaaa 2641 cacctggcta cccagacccc tcgtccccac aggtcaaccc acaatacaac ccgcagacgc 2701 cagggacgcc ggccatgtac aacacagacc agttctctcc ctatgctgcc ccctccccac 2761 aaggttccta ccagcccagc cccagccccc agagctacca ccaggtggcg ccaagcccag 2821 caggctacca gaatacccac tccccagcca gctaccaccc tacaccgtcg cccatggcct 2881 atcaggctag ccccagcccg agccccgttg gctacagtcc tatgacacct ggagctccct 2941 cccctggtgg ctacaaccca cacacgccag gctcaggcat cgagcagaac tccagcgact 3001 gggtaaccac tgacattcag gtgaaggtgc gggacaccta cctggataca caggtggtgg 3061 gacagacagg tgtcatccgc agtgtcacgg ggggcatgtg ctctgtgtac ctgaaggaca 3121 gtgagaaggt tgtcagcatt tccagtgagc acctggagcc tatcaccccc accaagaaca 3181 acaaggtgaa agtgatcctg ggcgaggatc gggaagccac gggcgtccta ctgagcattg 3241 atggtgagga tggcattgtc cgtatggacc ttgatgagca gctcaagatc ctcaacctcc 3301 gcttcctggg gaagctcctg gaagcctgcc caactttctt gtacaaagtg gttggtaagc 3361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3421 ggcgcgttaa gtcgacaatc aacctctgga tta