Construct: ORF ccsbBroad304_07034
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005320.1_s304c1, BRDN0000400222
- Derived from:
- ccsbBroadEn_07034
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBCE (6905)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07034
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6905 | TBCE | tubulin folding cofactor E | NM_001079515.3 | 99.9% | 99.8% | 20C>G |
| 2 | human | 6905 | TBCE | tubulin folding cofactor E | NM_003193.5 | 99.9% | 99.8% | 20C>G |
| 3 | human | 6905 | TBCE | tubulin folding cofactor E | NM_001287801.2 | 91.1% | 91% | 20C>G;659_811del |
| 4 | human | 6905 | TBCE | tubulin folding cofactor E | NM_001287802.1 | 77.9% | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgacactttg acaggggatg tcattggtcg aagagttgaa gttaatggag 121 aacatgcaac agtacgtttt gctggtgttg tccctcccgt ggcaggaccc tggttaggag 181 tagaatggga caatcccgag agaggaaagc atgatgggag ccacgaaggg actgtgtatt 241 ttaaatgcag gcacccgaca ggaggatcct ttattcgtcc gaacaaggta aattttggaa 301 cagactttct tactgcaatt aagaaccgct atgtgttaga agatggacca gaggaagata 361 gaaaagagca aattgttaca attggaaata aacctgtgga gactatcggt tttgactcta 421 ttatgaaaca gcaaagtcag ctgagcaagt tgcaagaagt ttctctgagg aactgtgcag 481 taagttgtgc tggtgaaaaa ggaggagttg ctgaagcatg tcctaatatc agaaaggtag 541 atttgtcaaa aaacctgttg tcatcatggg atgaagtgat acacattgct gatcagctca 601 gacacctgga agtccttaat gtcagtgaaa ataaactaaa atttccctcc ggttcagtat 661 taactggaac gctttctgta ctgaaggttt tagtcctcaa tcaaacagga ataacgtggg 721 ctgaggtgct gcggtgtgtc gcggggtgcc caggcctgga ggaactctac cttgagtcta 781 acaacatttt catttccgaa aggccaacag atgttctcca gacagtcaag ttattagatc 841 tttcctctaa tcaattaatt gatgaaaatc agctgtatct gatagcccac ctgcccaggt 901 tagaacaatt aatcctctct gacactggaa tttcttctct acattttccg gatgctggaa 961 ttgggtgcaa aacgtccatg ttcccatcct tgaagtacct ggtagtaaac gacaatcaga 1021 tatcacaatg gtcgtttttc aatgagctag agaagttacc aagtctacgg gctttgtcct 1081 gcctaagaaa ccccctgacc aaagaggaca aagaagcaga gacggcgcga ctactcatta 1141 tcgccagcat tggccagctg aagacgctga acaaatgtga gattctcccc gaggagaggc 1201 ggagagctga gcttgactac cgaaaagctt ttggaaatga gtggaaacag gctggtggac 1261 ataaggatcc ggaaaaaaac agactcagcg aagaattcct cacagcccat cccagatacc 1321 agttcctctg cctgaaatat ggtgcacctg aagattggga actcaaaaca cagcaaccac 1381 ttatgctgaa aaaccagcta ctaacactga agataaaata ccctcatcaa cttgatcaga 1441 aagtcctgga gaaacaactg ccgggctcca tgacaattca aaaggtgaag ggattgctgt 1501 cacgtcttct caaagttcct gtgtcagacc ttctgttgtc ctatgaaagt cccaaaaagc 1561 cgggcagaga aatcgagctg gaaaatgacc taaagtcatt acagttttat tctgtggaaa 1621 atggagattg tctattagtg cgatggtgcc caacttcctt gtacaaagtg gttggtaagc 1681 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1741 ggcgcgttaa gtcgacaatc aacctctgga tta