Construct: ORF ccsbBroad304_07118

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011710.1_s304c1, BRDN0000410160
Derived from:
ccsbBroadEn_07118
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
UGDH (7358)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07118
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7358 UGDH UDP-glucose 6-dehydrogenase NM_003359.4 99.8% .9% 30C>T;399A>G
2 human 7358 UGDH UDP-glucose 6-dehydrogenase XM_005262667.3 97.3% 1.3% 1_39del;69C>T;438A>G
3 human 7358 UGDH UDP-glucose 6-dehydrogenase NM_001184700.2 86.3% 1.1% 30C>T;264_265ins201
4 human 7358 UGDH UDP-glucose 6-dehydrogenase NM_001184701.2 80.2% 2.5% 0_1ins291;108A>G
5 mouse 22235 Ugdh UDP-glucose dehydrogenase NM_009466.2 89.7% .7% (many diffs)
6 mouse 22235 Ugdh UDP-glucose dehydrogenase XM_006503862.3 89.7% .7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
58
ORF end:
160
ORF length:
102
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaatg
61ttggcatgtt tgaaattaag aagatctgtt gcattggtgc aggctatgtt ggaggaccca
121catgtagtgt cattgctcat atgtgtcctg aaatcagggt aacggttgtt gatgtcaatg
181aatcaagaat caatgcgtgg aattctccta cacttcctat ttatgagcca ggactaaaag
241aagtggtaga atcctgtcga ggaaaaaatc tttttttttc taccaatatt gatgatgcca
301tcaaagaagc tgatcttgta tttatttctg tgaatactcc aacaaaaacc tatggaatgg
361ggaaaggccg ggcagcagat ctgaagtata ttgaagcttg tgctagacgc attgtgcaaa
421actcaaatgg gtacaaaatt gtgactgaga aaagcacagt tccggtgcgg gcagcagaaa
481gtatccgtcg catatttgat gcaaacacaa aacccaactt gaatttacag gtgctgtcca
541accctgagtt tctggcagag ggaacagcca tcaaggacct aaagaaccca gacagagtac
601tgattggagg ggatgaaact ccagagggcc agagagctgt gcaggccctg tgtgctgtat
661atgagcactg ggttcccaga gaaaagatcc tcaccactaa tacttggtct tcagagcttt
721ccaaactggc agcaaatgct tttcttgccc agagaataag cagcattaac tccataagtg
781ctctgtgtga agcaacagga gctgatgtag aagaggtagc aacagcgatt ggaatggacc
841agagaattgg aaacaagttt ctaaaagcca gtgttgggtt tggtgggagc tgtttccaaa
901aggatgttct gaatttggtt tatctctgtg aggctctgaa tttgccagaa gtagctcgtt
961attggcagca ggtcatagac atgaatgact accagaggag gaggtttgct tcccggatca
1021tagatagtct gtttaataca gtaactgata agaagatagc tattttggga tttgcattca
1081aaaaggacac tggtgataca agagaatctt ctagtatata tattagcaaa tatttgatgg
1141atgaaggtgc acatctacat atatatgatc caaaagtacc tagggaacaa atagttgtgg
1201atctttctca tccaggtgtt tcagaggatg accaagtgtc ccggctcgtg accatttcca
1261aggatccata tgaagcatgt gatggtgccc atgctgttgt tatttgcact gagtgggaca
1321tgtttaagga attggattat gaacgcattc ataaaaaaat gctaaagcca gcctttatct
1381tcgatggacg gcgtgtcctg gatgggctcc acaatgaact acaaaccatt ggcttccaga
1441ttgaaacaat tggcaaaaag gtgtcttcaa agagaattcc atatgctcct tctggtgaaa
1501ttccgaagtt tagtcttcaa gatccaccta acaagaaacc taaagtgtac ccaactttct
1561tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1621aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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