Construct: ORF ccsbBroad304_07130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011304.1_s304c1, BRDN0000462216
- Derived from:
- ccsbBroadEn_07130
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VLDLR (7436)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07130
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7436 | VLDLR | very low density lipoprotei... | NM_001018056.3 | 99.9% | 100% | 921C>T |
| 2 | human | 7436 | VLDLR | very low density lipoprotei... | NM_003383.5 | 96.7% | 96.6% | 921C>T;2252_2335del |
| 3 | human | 7436 | VLDLR | very low density lipoprotei... | NM_001322226.2 | 95.1% | 95% | 325_326ins123;798C>T |
| 4 | human | 7436 | VLDLR | very low density lipoprotei... | NM_001322225.2 | 92% | 91.8% | 325_326ins123;798C>T;2129_2212del |
| 5 | human | 7436 | VLDLR | very low density lipoprotei... | XR_001746373.2 | 69.3% | (many diffs) | |
| 6 | human | 7436 | VLDLR | very low density lipoprotei... | XR_002956805.1 | 67.7% | (many diffs) | |
| 7 | mouse | 22359 | Vldlr | very low density lipoprotei... | NM_001161420.1 | 91% | 96.5% | (many diffs) |
| 8 | mouse | 22359 | Vldlr | very low density lipoprotei... | NM_013703.2 | 88.1% | 93.3% | (many diffs) |
| 9 | mouse | 22359 | Vldlr | very low density lipoprotei... | XM_006526917.1 | 88.1% | 93.3% | (many diffs) |
| 10 | mouse | 22359 | Vldlr | very low density lipoprotei... | XM_006526918.2 | 88.1% | 93.3% | (many diffs) |
| 11 | mouse | 22359 | Vldlr | very low density lipoprotei... | NM_001347441.1 | 86.4% | 91.9% | (many diffs) |
| 12 | mouse | 22359 | Vldlr | very low density lipoprotei... | XM_006526919.2 | 83.6% | 88.8% | (many diffs) |
| 13 | mouse | 22359 | Vldlr | very low density lipoprotei... | XM_006526920.2 | 77.5% | 82.1% | (many diffs) |
| 14 | mouse | 22359 | Vldlr | very low density lipoprotei... | XR_386457.2 | 47.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2604
- ORF length:
- 2535
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcacgtcc gcgctctggg cgctctggct gctgctcgcg ctgtgctggg 121 cgccccggga gagcggcgcc accggaaccg ggagaaaagc caaatgtgaa ccctcccaat 181 tccagtgcac aaatggtcgc tgtattacgc tgttgtggaa atgtgatggg gatgaagact 241 gtgttgacgg cagtgatgaa aagaactgtg taaagaagac gtgtgctgaa tctgacttcg 301 tgtgcaacaa tggccagtgt gttcccagcc gatggaagtg tgatggagat cctgactgcg 361 aagatggttc agatgaaagc ccagaacagt gccatatgag aacatgccgc atacatgaaa 421 tcagctgtgg cgcccattct actcagtgta tcccagtgtc ctggagatgt gatggtgaaa 481 atgattgtga cagtggagaa gatgaagaaa actgtggcaa tataacatgt agtcccgacg 541 agttcacctg ctccagtggc cgctgcatct ccaggaactt tgtatgcaat ggccaggatg 601 actgcagcga tggcagtgat gagctggact gtgccccgcc aacctgtggc gcccatgagt 661 tccagtgcag cacctcctcc tgcatcccca tcagctgggt atgcgacgat gatgcagact 721 gctccgacca atctgatgag tccctggagc agtgtggccg tcagccagtc atacacacca 781 agtgtccagc cagcgaaatc cagtgcggct ctggcgagtg catccataag aagtggcgat 841 gtgatgggga ccctgactgc aaggatggca gtgatgaggt caactgtccc tctcgaactt 901 gccgacctga ccaatttgaa tgtgaggatg gcagctgcat ccatggcagc aggcagtgta 961 atggtatccg agactgtgtc gatggttctg atgaagtcaa ctgcaaaaat gtcaatcagt 1021 gcttgggccc tggaaaattc aagtgcagaa gtggagaatg catagatatc agcaaagtat 1081 gtaaccagga gcaggactgc agggactgga gtgatgagcc cctgaaagag tgtcatataa 1141 acgaatgctt ggtaaataat ggtggatgtt ctcatatctg caaagaccta gttataggct 1201 acgagtgtga ctgtgcagct gggtttgaac tgatagatag gaaaacctgt ggagatattg 1261 atgaatgcca aaatccagga atctgcagtc aaatttgtat caacttaaaa ggcggttaca 1321 agtgtgaatg tagtcgtggc tatcaaatgg atcttgctac tggcgtgtgc aaggcagtag 1381 gcaaagagcc aagtctgatc ttcactaatc gaagagacat caggaagatt ggcttagaga 1441 ggaaagaata tatccaacta gttgaacagc taagaaacac tgtggctctc gatgctgaca 1501 ttgctgccca gaaactattc tgggccgatc taagccaaaa ggctatcttc agtgcctcaa 1561 ttgatgacaa ggttggtaga catgttaaaa tgatcgacaa tgtctataat cctgcagcca 1621 ttgctgttga ttgggtgtac aagaccatct actggactga tgcggcttct aagactattt 1681 cagtagctac cctagatgga accaagagga agttcctgtt taactctgac ttgcgagagc 1741 ctgcctccat agctgtggac ccactgtctg gctttgttta ctggtcagac tggggtgaac 1801 cagctaaaat agaaaaagca ggaatgaatg gattcgatag acgtccactg gtgacagcgg 1861 atatccagtg gcctaacgga attacacttg accttataaa aagtcgcctc tattggcttg 1921 attctaagtt gcacatgtta tccagcgtgg acttgaatgg ccaagatcgt aggatagtac 1981 taaagtctct ggagttccta gctcatcctc ttgcactaac aatatttgag gatcgtgtct 2041 actggataga tggggaaaat gaagcagtct atggtgccaa taaattcact ggatcagagc 2101 tagccactct agtcaacaac ctgaatgatg cccaagacat cattgtctat catgaacttg 2161 tacagccatc aggtaaaaat tggtgtgaag aagacatgga gaatggagga tgtgaatacc 2221 tatgcctgcc agcaccacag attaatgatc actctccaaa atatacctgt tcctgtccca 2281 gtgggtacaa tgtagaggaa aatggccgag actgtcaaag gatcaatgtg accacagcag 2341 tatcagaggt cagtgttccc ccaaaaggga cttctgccgc atgggccatt cttcctctct 2401 tgctcttagt gatggcagca gtaggtggct acttgatgtg gcggaattgg caacacaaga 2461 acatgaaaag catgaacttt gacaatcctg tgtacttgaa aaccactgaa gaggacctct 2521 ccatagacat tggtagacac agtgcttctg ttggacacac gtacccagca atatcagttg 2581 taagcacaga tgatgatcta gctttgccaa ctttcttgta caaagtggtt ggtaagccta 2641 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2701 gcgttaagtc gacaatcaac ctctggatta