Construct: ORF ccsbBroad304_07212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005477.1_s304c1, BRDN0000407222
- Derived from:
- ccsbBroadEn_07212
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM10 (8241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07212
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_152856.3 | 99.9% | 99.8% | 14G>N |
| 2 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204466.2 | 99.8% | 99.7% | 14G>N;829_831delGTG |
| 3 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452462.1 | 96% | 95.9% | 14G>N;829_831delGTG;1341_1442del |
| 4 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272679.4 | 92.8% | 92.8% | 1_195del;209G>N |
| 5 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272678.4 | 92.7% | 92.7% | 1_195del;209G>N;1024_1026delGTG |
| 6 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204467.2 | 91.6% | 91.6% | 14G>N;202_432del |
| 7 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_005676.5 | 91.5% | 91.5% | 14G>N;202_432del;1060_1062delGTG |
| 8 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452461.1 | 89.4% | 89.3% | (many diffs) |
| 9 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452460.1 | 88.4% | 88.3% | 14G>N;202_432del;1569_1670del |
| 10 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452459.1 | 88.3% | 88.2% | (many diffs) |
| 11 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272677.4 | 85.6% | 85.6% | 1_195del;209G>N;397_627del |
| 12 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204468.1 | 85.5% | 85.5% | (many diffs) |
| 13 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_017029885.1 | 85.2% | 85.2% | 0_1ins378 |
| 14 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_017029884.2 | 85.1% | 85.1% | 0_1ins378;451_453delGTG |
| 15 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452458.1 | 82.8% | 82.7% | (many diffs) |
| 16 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452457.1 | 82.7% | 82.7% | (many diffs) |
| 17 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452463.1 | 81.8% | 81.8% | 0_1ins378;451_453delGTG;963_1064del |
| 18 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452464.1 | 81.8% | 81.8% | 0_1ins378;451_453delGTG;963_1064del |
| 19 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452465.1 | 69.5% | 69.5% | 0_1ins705;124_126delGTG;636_737del |
| 20 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527619.3 | 89.3% | 95.7% | (many diffs) |
| 21 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_001167776.1 | 89.2% | 95.6% | (many diffs) |
| 22 | mouse | 433287 | Gm15455 | predicted gene 15455 | NM_001161816.1 | 88.2% | 92.6% | (many diffs) |
| 23 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_001167775.1 | 81.9% | 87.8% | (many diffs) |
| 24 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527618.3 | 81.9% | 87.8% | (many diffs) |
| 25 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_145627.2 | 81.8% | 87.7% | (many diffs) |
| 26 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527620.3 | 80.4% | 86.1% | (many diffs) |
| 27 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527621.3 | 75.6% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2622
- ORF length:
- 2556
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatgaaana cgtggtggtc gtggtgacag gactggccgc tatggagcca 121 ctgaccgctc gcaggatgat ggtggggaga accgcagccg agaccacgac taccgggaca 181 tggactaccg ttcatatcct cgcgagtatg gcagccagga gggcaagcat gactatgacg 241 actcatctga ggagcagagt gcggagatcc gtggccagct gcagtcgcac ggcgtgcaag 301 cacgggaggt tcggctgatg cggaacaaat cttcaggtca gagccggggc ttcgccttcg 361 tcgagtttag tcacttgcag gacgctacac gatggatgga agccaatcag cactccctca 421 acatcctggg ccagaaggtg tcgatgcact acagtgaccc caagcccaag atcaatgagg 481 actggctgtg caataagtgt ggcgtccaga acttcaaacg ccgagagaag tgcttcaaat 541 gtggcgtgcc caagtcagag gcagagcaga agctgcccct cggcacgagg ctggatcagc 601 agacactgcc actgggtggc cgggagctga gccagggcct gcttcccctg ccgcagccct 661 accaggccca gggagtcctg gcctcccaag ccctgtcaca gggctcggag ccaagctcag 721 agaacgccaa tgacaccatc attttgcgca acctgaaccc acacagcacc atggattcca 781 tcctgggggc cctggcaccc tacgcggtgc tgtcctcctc caacgtgcgc gtcataaagg 841 acaagcagac ccaactgaac cgcggctttg ccttcatcca gctctccacc atcgaggcag 901 cccagctgct gcagatcctg caggccctgc acccaccact cactatcgac ggcaagacca 961 tcaatgttga gtttgccaag ggttctaaga gggacatggc ctccaatgaa ggcagtcgca 1021 tcagtgctgc ctctgtggcc agcactgcca ttgctgcggc ccagtgggcc atctcacagg 1081 cctcccaagg tggggagggt acctgggcca cctccgagga gccgccggtc gactacagct 1141 actaccaaca ggatgagggc tatggcaaca gccagggcac agagtcttcc ctctatgccc 1201 atggctacct caagggcacc aagggccctg gcatcactgg aaccaaaggg gatcccactg 1261 gagcaggtcc cgaggcctcc ctagagcctg gggccgactc tgtgtcgatg caggctttct 1321 ctcgcgccca gcctggtgct gctcctggca tctaccaaca atcagccgag gcgagcagta 1381 gccagggcac tgctgccaac agccagtcgt ataccatcat gtcacccgct gtgctcaaat 1441 ctgagctcca gagccctacc catcctagtt ctgctctccc accggctacc agccccactg 1501 cccaggaatc ctacagccag taccctgttc ccgacgtctc tacctaccag tacgatgaga 1561 cctccggcta ctactatgac ccccagaccg gcctctacta tgaccccaac tcccagtatt 1621 actacaatgc tcagagccag cagtacctgt actgggatgg ggagaggcgg acctatgttc 1681 ccgccctgga gcagtcggcc gacggacata aggagacagg ggcaccctcg aaggagggca 1741 aagagaagaa ggagaagcac aagaccaaga cagctcaaca gattgccaag gacatggaac 1801 gctgggcccg cagtctcaac aaacaaaaag aaaacttcaa aaatagcttc cagcctatca 1861 gctccctgcg agatgacgag aggcgggagt cagccactgc agatgctggc tatgccatcc 1921 tcgagaagaa gggagcacta gccgagagac agcacaccag catggatctc ccgaaattgg 1981 ccagtgacga ccgcccaagc cctccgcgag gactggtggc agcctacagc ggggagagtg 2041 acagtgagga ggagcaggag cgtgggggcc ctgagcggga ggagaagctc accgactggc 2101 agaagctggc ctgtctgctc tgccgacgcc agttccccag caaagaggcg ctcatccggc 2161 accagcagct ctcagggctc cacaagcaaa accttgagat tcaccggcga gcccacttgt 2221 cagaaaacga gctagaagca ctagagaaga atgacatgga gcaaatgaag taccgggacc 2281 gtgcagctga acgcagagaa aagtatggca tccccgagcc gccagagccc aagaggagga 2341 agtacggcgg catatccaca gcctctgtag acttcgagca gcctactcgg gacgggctgg 2401 gcagtgacaa cattggcagt cggatgctgc aggccatggg ctggaaagag ggcagcggcc 2461 tgggccgcaa gaagcagggc attgtaacgc ctatcgaggc ccaaacacgg gtgcggggct 2521 ccggcctggg tgcacggggc agctcctacg gggtcacctc aaccgagtcc tacaaggaga 2581 cactgcacaa gacaatggtg acccgcttca acgaggccca gtgcccaact ttcttgtaca 2641 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2701 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta