Construct: ORF ccsbBroad304_07218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014129.1_s304c1, BRDN0000400302
- Derived from:
- ccsbBroadEn_07218
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAP1 (8314)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07218
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8314 | BAP1 | BRCA1 associated protein 1 | NM_004656.4 | 99.9% | 100% | 366C>T |
| 2 | human | 8314 | BAP1 | BRCA1 associated protein 1 | XM_011534152.2 | 97.8% | 97.9% | 366C>T;1844_1845ins45 |
| 3 | human | 8314 | BAP1 | BRCA1 associated protein 1 | XM_011534150.3 | 97.8% | 96.8% | (many diffs) |
| 4 | human | 8314 | BAP1 | BRCA1 associated protein 1 | XM_017007303.2 | 97.4% | 97.5% | 366C>T;658_659ins54 |
| 5 | human | 8314 | BAP1 | BRCA1 associated protein 1 | XM_011534149.3 | 96.8% | 96.9% | 366C>T;1889_1957del |
| 6 | human | 8314 | BAP1 | BRCA1 associated protein 1 | XM_011534151.3 | 94.5% | 94.5% | 366C>T;658_659ins54;1835_1903del |
| 7 | mouse | 104416 | Bap1 | Brca1 associated protein 1 | NM_027088.2 | 88.5% | 93.4% | (many diffs) |
| 8 | mouse | 104416 | Bap1 | Brca1 associated protein 1 | XR_383122.3 | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2253
- ORF length:
- 2187
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa taagggctgg ctggagctgg agagcgaccc aggcctcttc accctgctcg 121 tggaagattt cggtgtcaag ggggtgcaag tggaggagat ctacgacctt cagagcaaat 181 gtcagggccc tgtatatgga tttatcttcc tgttcaaatg gatcgaagag cgccggtccc 241 ggcgaaaggt ctctaccttg gtggatgata cgtccgtgat tgatgatgat attgtgaata 301 acatgttctt tgcccaccag ctgataccca actcttgtgc aactcatgcc ttgctgagcg 361 tgctcctgaa ctgcagcagc gtggacctgg gacccaccct gagtcgcatg aaggacttca 421 ccaagggttt tagccctgag agcaaaggat atgcgattgg caatgccccg gagttggcca 481 aggcccataa tagccatgcc aggcccgagc cacgccacct ccctgagaag cagaatggcc 541 ttagtgcagt gcggaccatg gaggcgttcc actttgtcag ctatgtgcct atcacaggcc 601 ggctctttga gctggatggg ctgaaggtct accccattga ccatgggccc tggggggagg 661 acgaggagtg gacagacaag gcccggcggg tcatcatgga gcgtatcggc ctcgccactg 721 caggggagcc ctaccacgac atccgcttca acctgatggc agtggtgccc gaccgcagga 781 tcaagtatga ggccaggctg catgtgctga aggtgaaccg tcagacagta ctagaggctc 841 tgcagcagct gataagagta acacagccag agctgattca gacccacaag tctcaagagt 901 cacagctgcc tgaggagtcc aagtcagcca gcaacaagtc cccgctggtg ctggaagcaa 961 acagggcccc tgcagcctct gagggcaacc acacagatgg tgcagaggag gcggctggtt 1021 catgcgcaca agccccatcc cacagccctc ccaacaaacc caagctagtg gtgaagcctc 1081 caggcagcag cctcaatggg gttcacccca accccactcc cattgtccag cggctgccgg 1141 cctttctaga caatcacaat tatgccaagt cccccatgca ggaggaagaa gacctggcgg 1201 caggtgtggg ccgcagccga gttccagtcc gcccacccca gcagtactca gatgatgagg 1261 atgactatga ggatgacgag gaggatgacg tgcagaacac caactctgcc cttaggtata 1321 aggggaaggg aacagggaag ccaggggcat tgagcggttc tgctgatggg caactgtcag 1381 tgctgcagcc caacaccatc aacgtcttgg ctgagaagct caaagagtcc cagaaggacc 1441 tctcaattcc tctgtccatc aagactagca gcggggctgg gagtccggct gtggcagtgc 1501 ccacacactc gcagccctca cccaccccca gcaatgagag tacagacacg gcctctgaga 1561 tcggcagtgc tttcaactcg ccactgcgct cgcctatccg ctcagccaac ccgacgcggc 1621 cctccagccc tgtcacctcc cacatctcca aggtgctttt tggagaggat gacagcctgc 1681 tgcgtgttga ctgcatacgc tacaaccgtg ctgtccgtga tctgggtcct gtcatcagca 1741 caggcctgct gcacctggct gaggatgggg tgctgagtcc cctggcgctg acagagggtg 1801 ggaagggttc ctcgccctcc atcagaccaa tccaaggcag ccaggggtcc agcagcccag 1861 tggagaagga ggtcgtggaa gccacggaca gcagagagaa gacggggatg gtgaggcctg 1921 gcgagccctt gagtggggag aaatactcac ccaaggagct gctggcactg ctgaagtgtg 1981 tggaggctga gattgcaaac tatgaggcgt gcctcaagga ggaggtagag aagaggaaga 2041 agttcaagat tgatgaccag agaaggaccc acaactacga tgagttcatc tgcaccttta 2101 tctccatgct ggctcaggaa ggcatgctgg ccaacctagt ggagcagaac atctccgtgc 2161 ggcggcgcca aggggtcagc atcggccggc tccacaagca gcggaagcct gaccggcgga 2221 aacgctctcg cccctacaag gccaagcgcc agtgcccaac tttcttgtac aaagtggttg 2281 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2341 accggtggcg cgttaagtcg acaatcaacc tctggatta