Construct: ORF ccsbBroad304_07221
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017373.1_s304c1, BRDN0000461727
- Derived from:
- ccsbBroadEn_07221
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FZD6 (8323)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07221
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164615.2 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_003506.4 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164616.2 | 95.2% | 95.1% | (many diffs) |
| 4 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001317796.2 | 56.1% | 55.2% | (many diffs) |
| 5 | human | 8323 | FZD6 | frizzled class receptor 6 | NR_133921.2 | 51.7% | (many diffs) | |
| 6 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_011245455.2 | 57% | 59% | (many diffs) |
| 7 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_017316431.1 | 57% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2187
- ORF length:
- 2118
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatgttt acatttttgt tgacgtgtat ttttctaccc ctcctaagag 121 ggcacagtct cttcacctgt gaaccaatta ctgttcccag atgtatgaaa atggcctaca 181 acatgacgtt tttccctaat ctgatgggtc attatgacca gagtattgcc gcggtggaaa 241 tggagcattt tcttcctctc gcaaatctgg aatgttcacc aaacattgaa actttcctct 301 gcaaagcatt tgtaccaacc tgcatagaac aaattcatgt ggttccacct tgtcgtaaac 361 tttgtgagaa agtatattct gattgcaaaa aattaattga cacttttggg atccgatggc 421 ctgaggagct tgaatgtgac agattacaat actgtgatga gactgttcct gtaacttttg 481 atccacacac agaatttctt ggtcctcaga agaaaacaga acaagtccaa agagacattg 541 gattttggtg tccaaggcat cttaagactt ctgggggaca aggatataag tttctgggaa 601 ttgaccagtg tgcgcctcca tgccccaaca tgtattttaa aagtgatgag ctagagtttg 661 caaaaagttt tattggaaca gtttcaatat tttgtctttg tgcaactctg ttcacattcc 721 ttactttttt aattgatgtt agaagattca gatacccaga gagaccaatt atatattact 781 ctgtctgtta cagcattgta tctcttatgt acttcattgg atttttgctg ggcgatagca 841 cagcctgcaa taaggcagat gagaagctag aacttggtga cactgttgtc ctaggctctc 901 aaaataaggc ttgcaccgtt ttgttcatgc ttttgtattt tttcacaatg gctggcactg 961 tgtggtgggt gattcttacc attacttggt tcttagctgc aggaagaaaa tggagttgtg 1021 aagccatcga gcaaaaagca gtgtggtttc atgctgttgc atggggaaca ccaggtttcc 1081 tgactgttat gcttcttgct ctgaacaaag ttgaaggaga caacattagt ggagtttgct 1141 ttgttggcct ttatgacctg gatgcttctc gctactttgt actcttgcca ctgtgccttt 1201 gtgtgtttgt tgggctctct cttcttttag ctggcattat ttccttaaat catgttcgac 1261 aagtcataca acatgatggc cggaaccaag aaaaactaaa gaaatttatg attcgaattg 1321 gagtcttcag cggcttgtat cttgtgccat tagtgacact tctcggatgt tacgtctatg 1381 agcaagtgaa caggattacc tgggagataa cttgggtctc tgatcattgt cgtcagtacc 1441 atatcccatg tccttatcag gcaaaagcaa aagctcgaca agaattggct ttatttatga 1501 taaaatacct gatgacatta attgttggca tctctgctgt cttctgggtt ggaagcaaaa 1561 agacatgcac agaatgggct gggtttttta aacgaaatcg caagagagat ccaatcagtg 1621 aaagtcgaag agtactacag gaatcatgtg agtttttctt aaagcacaat tctaaagtta 1681 aacacaaaaa gaagcactat aaaccaagtt cacacaagct gaaggtcatt tccaaatcca 1741 tgggaaccag cacaggagct acagcaaatc atggcacttc tgcagtagca attactagcc 1801 atgattacct aggacaagaa actttgacag aaatccaaac ctcaccagaa acatcaatga 1861 gagaggtgaa agcggacgga gctagcaccc ccaggttaag agaacaggac tgtggtgaac 1921 ctgcctcgcc agcagcatcc atctccagac tctctgggga acaggtcgac gggaagggcc 1981 aggcaggcag tgtatctgaa agtgcgcgga gtgaaggaag gattagtcca aagagtgata 2041 ttactgacac tggcctggca cagagcaaca atttgcaggt ccccagttct tcagaaccaa 2101 gcagcctcaa aggttccaca tctctgcttg ttcacccagt ttcaggagtg agaaaagagc 2161 agggaggtgg ttgtcattca gatactttgc caactttctt gtacaaagtg gttggtaagc 2221 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2281 ggcgcgttaa gtcgacaatc aacctctgga tta