Construct: ORF ccsbBroad304_07259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011825.1_s304c1, BRDN0000401264
- Derived from:
- ccsbBroadEn_07259
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMO4 (8543)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07259
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8543 | LMO4 | LIM domain only 4 | NM_001369491.1 | 99.5% | 99.3% | 488A>C;492C>T |
| 2 | human | 8543 | LMO4 | LIM domain only 4 | NM_006769.4 | 99.5% | 99.3% | 488A>C;492C>T |
| 3 | mouse | 16911 | Lmo4 | LIM domain only 4 | NM_001161769.1 | 95.3% | 99.3% | (many diffs) |
| 4 | mouse | 16911 | Lmo4 | LIM domain only 4 | NM_001161770.1 | 95.3% | 99.3% | (many diffs) |
| 5 | mouse | 16911 | Lmo4 | LIM domain only 4 | NM_010723.3 | 95.3% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gaatccgggc agcagctcgc agccgccccc ggtgacggcc ggctccctct 121 cctggaagcg gtgcgcaggc tgcgggggca agattgcgga ccgctttctg ctctatgcca 181 tggacagcta ttggcacagc cggtgcctca agtgctcctg ctgccaggcg cagctgggcg 241 acatcggcac gtcctgttac accaaaagtg gcatgatcct ttgcagaaat gactacatta 301 ggttatttgg aaatagcggt gcttgcagcg cttgcggaca gtcgattcct gcgagtgaac 361 tcgtcatgag ggcgcaaggc aatgtgtatc atcttaagtg ttttacatgc tctacctgcc 421 ggaatcgcct ggtcccggga gatcggtttc actacatcaa tggcagttta ttttgtgaac 481 atgatagacc tacagctctc atcaatggcc atttgaattc acttcagagc aatccactac 541 tgccagacca gacggtttgc tacccaactt tcttgtacaa agtggttggt aagcctatcc 601 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 661 ttaagtcgac aatcaacctc tggatta