Construct: ORF ccsbBroad304_07260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013702.1_s304c1, BRDN0000401814
- Derived from:
- ccsbBroadEn_07260
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGR5 (8549)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07260
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_003667.4 | 99.9% | 99.8% | 882G>A;1997T>C |
| 2 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277226.2 | 97.2% | 97.2% | 784_785ins72;810G>A;1925T>C |
| 3 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277227.2 | 91.9% | 91.9% | 426_427ins216;666G>A;1781T>C |
| 4 | human | 8549 | LGR5 | leucine rich repeat contain... | NR_110596.2 | 43.9% | (many diffs) | |
| 5 | mouse | 14160 | Lgr5 | leucine rich repeat contain... | NM_010195.2 | 84.6% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2790
- ORF length:
- 2721
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacacctcc cggctcggtg tgctcctgtc cttgcctgtg ctgctgcagc 121 tggcgaccgg gggcagctct cccaggtctg gtgtgttgct gaggggctgc cccacacact 181 gtcattgcga gcccgacggc aggatgttgc tcagggtgga ctgctccgac ctggggctct 241 cggagctgcc ttccaacctc agcgtcttca cctcctacct agacctcagt atgaacaaca 301 tcagtcagct gctcccgaat cccctgccca gtctccgctt cctggaggag ttacgtcttg 361 cgggaaacgc tctgacatac attcccaagg gagcattcac tggcctttac agtcttaaag 421 ttcttatgct gcagaataat cagctaagac acgtacccac agaagctctg cagaatttgc 481 gaagccttca atccctgcgt ctggatgcta accacatcag ctatgtgccc ccaagctgtt 541 tcagtggcct gcattccctg aggcacctgt ggctggatga caatgcgtta acagaaatcc 601 ccgtccaggc ttttagaagt ttatcggcat tgcaagccat gaccttggcc ctgaacaaaa 661 tacaccacat accagactat gcctttggaa acctctccag cttggtagtt ctacatctcc 721 ataacaatag aatccactcc ctgggaaaga aatgctttga tgggctccac agcctagaga 781 ctttagattt aaattacaat aaccttgatg aattccccac tgcaattagg acactctcca 841 accttaaaga actaggattt catagcaaca atatcaggtc gatacctgag aaagcatttg 901 taggcaaccc ttctcttatt acaatacatt tctatgacaa tcccatccaa tttgttggga 961 gatctgcttt tcaacattta cctgaactaa gaacactgac tctgaatggt gcctcacaaa 1021 taactgaatt tcctgattta actggaactg caaacctgga gagtctgact ttaactggag 1081 cacagatctc atctcttcct caaaccgtct gcaatcagtt acctaatctc caagtgctag 1141 atctgtctta caacctatta gaagatttac ccagtttttc agtctgccaa aagcttcaga 1201 aaattgacct aagacataat gaaatctacg aaattaaagt tgacactttc cagcagttgc 1261 ttagcctccg atcgctgaat ttggcttgga acaaaattgc tattattcac cccaatgcat 1321 tttccacttt gccatcccta ataaagctgg acctatcgtc caacctcctg tcgtcttttc 1381 ctataactgg gttacatggt ttaactcact taaaattaac aggaaatcat gccttacaga 1441 gcttgatatc atctgaaaac tttccagaac tcaaggttat agaaatgcct tatgcttacc 1501 agtgctgtgc atttggagtg tgtgagaatg cctataagat ttctaatcaa tggaataaag 1561 gtgacaacag cagtatggac gaccttcata agaaagatgc tggaatgttt caggctcaag 1621 atgaacgtga ccttgaagat ttcctgcttg actttgagga agacctgaaa gcccttcatt 1681 cagtgcagtg ttcaccttcc ccaggcccct tcaaaccctg tgaacacctg cttgatggct 1741 ggctgatcag aattggagtg tggaccatag cagttctggc acttacttgt aatgctttgg 1801 tgacttcaac agttttcaga tcccctctgt acatttcccc cattaaactg ttaattgggg 1861 tcatcgcagc agtgaacatg ctcacgggag tctccagtgc cgtgctggct ggtgtggatg 1921 cgttcacttt tggcagcttt gcacgacatg gtgcctggtg ggagaatggg gttggttgcc 1981 atgtcattgg ttttttgtcc atttttgctt cagaatcatc tgttttcctg cttactctgg 2041 cagccctgga gcgtgggttc tctgcgaaat attctgcaaa atttgaaacg aaagctccat 2101 tttctagcct gaaagtaatc attttgctct gtgccctgct ggccttgacc atggccgcag 2161 ttcccctgct gggtggcagc aagtatggcg cctcccctct ctgcctgcct ttgccttttg 2221 gggagcccag caccatgggc tacatggtcg ctctcatctt gctcaattcc ctttgcttcc 2281 tcatgatgac cattgcctac accaagctct actgcaattt ggacaaggga gacctggaga 2341 atatttggga ctgctctatg gtaaaacaca ttgccctgtt gctcttcacc aactgcatcc 2401 taaactgccc tgtggctttc ttgtccttct cctctttaat aaaccttaca tttatcagtc 2461 ctgaagtaat taagtttatc cttctggtgg tagtcccact tcctgcatgt ctcaatcccc 2521 ttctctacat cttgttcaat cctcacttta aggaggatct ggtgagcctg agaaagcaaa 2581 cctacgtctg gacaagatca aaacacccaa gcttgatgtc aattaactct gatgatgtcg 2641 aaaaacagtc ctgtgactca actcaagcct tggtaacctt taccagctcc agcatcactt 2701 atgacctgcc tcccagttcc gtgccatcac cagcttatcc agtgactgag agctgccatc 2761 tttcctctgt ggcatttgtc ccatgtctct tgccaacttt cttgtacaaa gtggttggta 2821 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2881 ggtggcgcgt taagtcgaca atcaacctct ggatta