Construct: ORF ccsbBroad304_07281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012731.1_s304c1, BRDN0000409557
- Derived from:
- ccsbBroadEn_07281
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH4A1 (8659)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07281
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | NM_003748.4 | 99.8% | 99.6% | 1050G>C;1408G>A;1570C>N |
| 2 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | NM_170726.3 | 99.8% | 99.6% | 1050G>C;1408G>A;1570C>N |
| 3 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | NM_001319218.1 | 90.7% | 90.5% | (many diffs) |
| 4 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | NM_001161504.1 | 89.1% | 88.9% | (many diffs) |
| 5 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | XR_946786.1 | 47.3% | (many diffs) | |
| 6 | human | 8659 | ALDH4A1 | aldehyde dehydrogenase 4 fa... | XR_001737510.1 | 42.5% | (many diffs) | |
| 7 | mouse | 212647 | Aldh4a1 | aldehyde dehydrogenase 4 fa... | NM_175438.4 | 86.7% | 91.2% | (many diffs) |
| 8 | mouse | 212647 | Aldh4a1 | aldehyde dehydrogenase 4 fa... | XM_006538693.3 | 76% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gctgccggcg cccgcgctcc gccgcgccct gctgtcccgc ccctggaccg 121 gggccggcct gcggtggaag cacacctcct ccctgaaggt ggccaacgag cccgtcttag 181 ccttcacgca gggcagccct gagcgagatg ccctgcaaaa ggccttgaag gacctgaagg 241 gccggatgga agccatccca tgcgtggtgg gggatgagga ggtgtggacg tcggacgtgc 301 agtaccaagt gtcgcctttt aaccatggac ataaggtggc caagttctgt tatgcagaca 361 agagcctgct caacaaagcc attgaggctg ccctggctgc ccggaaagag tgggacctga 421 agcctattgc agaccgggcc cagatcttcc tgaaggcggc agacatgctg agtgggccgc 481 gcagggctga gatcctcgcc aagaccatgg tgggacaggg taagaccgtg atccaagcgg 541 agattgacgc tgcagcggaa ctcatcgact tcttccggtt caatgccaag tatgcggtgg 601 agctggaggg gcagcagccc atcagcgtgc ccccgagcac caacagcacg gtgtaccggg 661 gtctggaggg cttcgtggcg gccatctcgc cctttaactt cactgcaatc ggcggcaacc 721 tggcgggggc accggccctg atgggcaacg tggtcctatg gaagcccagt gacactgcca 781 tgctggccag ctatgctgtc taccgcatcc ttcgggaggc tggcctgccc cccaacatca 841 tccagtttgt gccagctgat gggcccctat ttggggacac tgtcaccagc tcagagcacc 901 tctgtggcat caacttcaca ggcagtgtgc ccaccttcaa acacctgtgg aagcaggtgg 961 cccagaacct ggaccggttc cacaccttcc cacgcctggc tggagagtgc ggcggaaaga 1021 acttccactt cgtgcaccgc tcggccgacg tggagagcgt ggtgagcggg accctccgct 1081 cagccttcga gtacggtggc cagaagtgtt ccgcctgctc gcgtctctac gtgccgcact 1141 cgctgtggcc gcagatcaaa gggcggctgc tggaggagca cagtcggatc aaagtgggcg 1201 accctgcaga ggattttggg accttcttct ctgcagtgat tgatgccaag tcctttgccc 1261 gtatcaagaa gtggctggag cacgcacgct cctcacccag cctcaccatc ctggccgggg 1321 gcaagtgtga tgactccgtg ggctactttg tggagccctg catcgtggag agcaaggacc 1381 ctcaggagcc catcatgaag gaggagatct tcgggcctgt actgtctgtg tacgtctacc 1441 cggatgacaa gtacaaggag acgctgcagc tgattgacag caccaccagc tatggcctca 1501 cgggggcagt gttctcccag gataaggacg tcgtgcagga ggccacaaag gtgctgagga 1561 atgctgccgg caacttctac atcaacgaca agtccactgg ctcgatagtg ggccagcagc 1621 cctttggggg ggccngagcc tctggaacca atgacaagcc agggggccca cactacatcc 1681 tgcgctggac gtcgccgcag gtcatcaagg agacacataa gcccctgggg gactggagct 1741 acgcgtacat gcagtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1801 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1861 cgacaatcaa cctctggatt a