Construct: ORF ccsbBroad304_07322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016331.1_s304c1, BRDN0000461602
- Derived from:
- ccsbBroadEn_07322
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGPL1 (8879)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07322
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | NM_003901.4 | 99.9% | 100% | 564C>T |
| 2 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | XM_005270263.1 | 99.9% | 100% | 564C>T |
| 3 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | XM_011540316.2 | 99.9% | 100% | 564C>T |
| 4 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | XM_011540317.1 | 99.9% | 100% | 564C>T |
| 5 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | XM_006718053.1 | 85.8% | 85.9% | 0_1ins240;324C>T |
| 6 | human | 8879 | SGPL1 | sphingosine-1-phosphate lya... | XM_011540318.1 | 85.8% | 85.9% | 0_1ins240;324C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1773
- ORF length:
- 1704
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctagcaca gaccttctga tgttgaaggc ctttgagccc tacttagaga 121 ttttggaagt atactccaca aaagccaaga attatgtaaa tggacattgc accaagtatg 181 agccctggca gctaattgca tggagtgtcg tgtggaccct gctgatagtc tggggatatg 241 agtttgtctt ccagccagag agtttatggt caaggtttaa aaagaaatgt tttaagctca 301 ccaggaagat gcccattatt ggtcgtaaga ttcaagacaa gttgaacaag accaaggatg 361 atattagcaa gaacatgtca ttcctgaaag tggacaaaga gtatgtgaaa gctttaccct 421 cccagggtct gagctcatct gctgttttgg agaaacttaa ggagtacagc tctatggacg 481 ccttctggca agaggggaga gcctctggaa cagtgtacag tggggaggag aagctcactg 541 agctccttgt gaaggcttat ggagattttg catggagtaa ccccctgcat ccagatatct 601 tcccaggact acgcaagata gaggcagaaa ttgtgaggat agcttgttcc ctgttcaatg 661 ggggaccaga ttcgtgtgga tgtgtgactt ctgggggaac agaaagcata ctgatggcct 721 gcaaagcata tcgggatctg gcctttgaga aggggatcaa aactccagaa attgtggctc 781 cccaaagtgc ccatgctgca tttaacaaag cagccagtta ctttgggatg aagattgtgc 841 gggtcccatt gacgaagatg atggaggtgg atgtgcgggc aatgagaaga gctatctcca 901 ggaacactgc catgctcgtc tgttctaccc cacagtttcc tcatggtgta atagatcctg 961 tccctgaagt ggccaagctg gctgtcaaat acaaaatacc ccttcatgtc gacgcttgtc 1021 tgggaggctt cctcatcgtc tttatggaga aagcaggata cccactggag cacccatttg 1081 atttccgggt gaaaggtgta accagcattt cagctgacac ccataagtat ggctatgccc 1141 caaaaggctc atcattggtg ttgtatagtg acaagaagta caggaactat cagttcttcg 1201 tcgatacaga ttggcagggt ggcatctatg cttccccaac catcgcaggc tcacggcctg 1261 gtggcattag cgcagcctgt tgggctgcct tgatgcactt cggtgagaac ggctatgttg 1321 aagctaccaa acagatcatc aaaactgctc gcttcctcaa gtcagaactg gaaaatatca 1381 aaggcatctt tgtttttggg aatccccaat tgtcagtcat tgctctggga tcccgtgatt 1441 ttgacatcta ccgactatca aacctgatga ctgctaaggg gtggaacttg aaccagttgc 1501 agttcccacc cagtattcat ttctgcatca cattactaca cgcccggaaa cgagtagcta 1561 tacaattcct aaaggacatt cgagaatctg tcactcaaat catgaagaat cctaaagcga 1621 agaccacagg aatgggtgcc atctatggca tggcccagac aactgttgac aggaatatgg 1681 ttgcagaatt gtcctcagtc ttcttggaca gcttgtacag caccgacact gtcacccagg 1741 gcagccagat gaatggttct ccaaaacccc acttgccaac tttcttgtac aaagtggttg 1801 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1861 accggtggcg cgttaagtcg acaatcaacc tctggatta