Transcript: Human XM_011540317.1

PREDICTED: Homo sapiens sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SGPL1 (8879)
Length:
4613
CDS:
132..1838

Additional Resources:

NCBI RefSeq record:
XM_011540317.1
NBCI Gene record:
SGPL1 (8879)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011540317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000294218 CCCGGAAACGAGTAGCTATAC pLKO_005 1606 CDS 100% 10.800 15.120 N SGPL1 n/a
2 TRCN0000078316 GATGCCCATTATTGGTCGTAA pLKO.1 371 CDS 100% 4.950 6.930 N SGPL1 n/a
3 TRCN0000078314 GCCCATTATTGGTCGTAAGAT pLKO.1 374 CDS 100% 5.625 4.500 N SGPL1 n/a
4 TRCN0000286833 GCCCATTATTGGTCGTAAGAT pLKO_005 374 CDS 100% 5.625 4.500 N SGPL1 n/a
5 TRCN0000078313 GCGTTTACATAACAGATGTTT pLKO.1 2235 3UTR 100% 5.625 4.500 N SGPL1 n/a
6 TRCN0000286758 GCGTTTACATAACAGATGTTT pLKO_005 2235 3UTR 100% 5.625 4.500 N SGPL1 n/a
7 TRCN0000294156 TGCTCTGGGATCCCGTGATTT pLKO_005 1484 CDS 100% 13.200 9.240 N SGPL1 n/a
8 TRCN0000078317 CCAGATATCTTCCCAGGACTA pLKO.1 654 CDS 100% 4.050 2.835 N SGPL1 n/a
9 TRCN0000078315 GCCAGATGAATGGTTCTCCAA pLKO.1 1807 CDS 100% 2.640 1.848 N SGPL1 n/a
10 TRCN0000286832 GCCAGATGAATGGTTCTCCAA pLKO_005 1807 CDS 100% 2.640 1.848 N SGPL1 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2585 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000157610 GTGGCATGATCTCAGCTCATT pLKO.1 2374 3UTR 100% 4.950 2.475 Y CCNJL n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2585 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011540317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07322 pDONR223 100% 99.9% 100% None 564C>T n/a
2 ccsbBroad304_07322 pLX_304 0% 99.9% 100% V5 564C>T n/a
3 TRCN0000477270 TAACCCGGAATTTCCTATACGATC pLX_317 22% 99.9% 100% V5 564C>T n/a
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