Construct: ORF ccsbBroad304_07327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007402.1_s304c1, BRDN0000406496
- Derived from:
- ccsbBroadEn_07327
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2S2 (8894)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07327
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_003908.5 | 99.8% | 99.6% | 531G>T |
| 2 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_001316363.2 | 98.9% | 98.7% | 294_295insAAGGATCTT;522G>T |
| 3 | human | 8894 | EIF2S2 | eukaryotic translation init... | XM_017028118.1 | 98.3% | 98.1% | 0_1insATGTCTGGGGACGAG;516G>T |
| 4 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_001316364.2 | 94.1% | 93.9% | 531G>T;683_684ins57 |
| 5 | mouse | 67204 | Eif2s2 | eukaryotic translation init... | NM_026030.2 | 92% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggggacgag atgatttttg atcctactat gagcaagaag aaaaagaaga 121 agaagaagcc ttttatgtta gatgaggaag gggataccca aacagaggaa acccagcctt 181 cagaaacaaa agaagtggag ccagagccaa ctgaggacaa ggatttggaa gctgatgaag 241 aggacactag gaaaaaagat gcttctgatg atctagatga cttgaacttc tttaatcaaa 301 agaaaaagaa gaaaaaaact aaaaagatat ttgatattga tgaagctgaa gaaggtgtaa 361 aggatcttaa gattgaaagt gatgttcaag aaccaactga accagaggat gaccttgaca 421 ttatgcttgg caataaaaag aagaaaaaga agaatgttaa gttcccagat gaggatgaaa 481 tactagagaa agatgaagct ctagaagatg aagacaacaa aaaagatgat ggtatctcat 541 tcagtaatca gacaggccct gcttgggcag gctcagaaag agactacaca tacgatgagc 601 tgctgaatcg agtgttcaac atcatgaggg aaaagaatcc agatatggtt gctggggaga 661 aaaggaaatt tgtcatgaaa cctccacaag tcgtccgagt aggaaccaag aaaacttctt 721 ttgtcaactt tacagatatc tgtaaactat tacatcgtca gcccaaacat ctccttgcat 781 ttttgttggc tgaattgggt acaagtggtt ctatagatgg taataaccaa cttgtaatca 841 aaggaagatt ccaacagaaa cagatagaaa atgtcttgag aagatatatc aaggaatatg 901 tcacttgtca cacatgccga tcaccggaca caatcctgca gaaggacaca cgactctatt 961 tcctacagtg cgaaacttgt cattctagat gttctgttgc cagtatcaaa accggcttcc 1021 aggctgtcac gggcaagcga gcacagctcc gtgccaaagc taactaccca actttcttgt 1081 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1141 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a