Construct: ORF ccsbBroad304_07352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011267.1_s304c1, BRDN0000400807
- Derived from:
- ccsbBroadEn_07352
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPSS1 (9061)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07352
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | NM_005443.5 | 99.9% | 100% | 36A>G |
| 2 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532400.2 | 96.6% | 96.6% | 0_1ins63 |
| 3 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532401.1 | 96.6% | 96.6% | 0_1ins63 |
| 4 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_011863.2 | 88% | 98.7% | (many diffs) |
| 5 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289477.1 | 84.8% | 95.3% | (many diffs) |
| 6 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289478.1 | 84.8% | 95.3% | (many diffs) |
| 7 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289479.1 | 77.8% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1938
- ORF length:
- 1872
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gatccccggg agcctgtgca agaaagtcaa gctgagcaat aacgcgcaga 121 actggggaat gcagagagca accaatgtca cctaccaagc ccatcatgtc agcaggaaca 181 agagaggtca ggtggtgggg accagaggtg gctttcgtgg ttgcacagtt tggctaacag 241 gcttgtctgg agcgggaaag actactgtga gcatggcctt ggaggagtac ctggtttgtc 301 atggtattcc atgctacact ctggatggtg acaatattcg tcaaggtctc aataaaaatc 361 ttggctttag tcctgaagac agagaagaga atgttcgacg catcgcagaa gttgctaaac 421 tgtttgcaga tgctggctta gtgtgcatca caagtttcat atcaccttac actcaggatc 481 gcaacaatgc aaggcaaatt catgaaggtg caagtttacc gttttttgaa gtatttgttg 541 atgctcctct gcatgtttgt gaacagaggg atgtcaaagg actctacaaa aaagcccggg 601 caggagaaat taaaggtttc actgggatcg attctgaata tgaaaagcca gaggcccctg 661 agttggtgct gaaaacagac tcctgtgatg taaatgactg tgtccagcaa gttgtggaac 721 ttctacagga acgggatatt gtacctgtgg atgcatctta tgaagtaaaa gaactatatg 781 tgccagaaaa taaacttcat ttggcaaaaa cagatgcgga aacattacca gcactgaaaa 841 ttaataaagt ggatatgcag tgggtgcagg ttttggcaga aggttgggca accccattga 901 atggctttat gagagagagg gagtacttgc agtgccttca ttttgattgt cttctggatg 961 gaggtgtcat taacttgtca gtacctatag ttctgactgc gactcatgaa gataaagaga 1021 ggctggacgg ctgtacagca tttgctctga tgtatgaggg ccgccgtgtg gccattcttc 1081 gcaatccaga gttttttgag cacaggaaag aggagcgctg tgccagacag tggggaacga 1141 catgcaagaa ccacccctat attaagatgg tgatggaaca aggagattgg ctgattggag 1201 gagatcttca agtcttggat cgagtttatt ggaatgatgg tcttgatcag tatcgtctta 1261 ctcctactga gctaaagcag aaatttaaag atatgaatgc tgatgctgtc tttgcatttc 1321 aactacgcaa cccagtgcac aatggacatg ccctgttaat gcaggatacc cataagcaac 1381 ttctagagag gggctaccgg cgccctgtcc tcctcctcca ccctctgggt ggctggacaa 1441 aggatgacga tgttcctttg atgtggcgta tgaagcagca tgctgcagtg ttggaggaag 1501 gagttctgaa tcctgagacg acagtggtgg ccatcttccc atctcccatg atgtatgctg 1561 gaccaactga ggtccagtgg cattgcagag cacggatggt tgcaggagcc aacttttaca 1621 ttgttggacg agaccctgct ggcatgcctc atccagaaac agggaaggat ctttatgagc 1681 caagtcatgg tgccaaagtg ctgacgatgg cccctggttt aatcactttg gaaatagttc 1741 cctttcgagt tgcagcttac aacaagaaaa agaagcgtat ggactactat gactctgaac 1801 accatgaaga ctttgaattt atttcaggaa cacgaatgcg caaacttgct cgagaaggcc 1861 agaaaccacc tgaaggtttc atggctccca aggcttggac cgtgctgaca gaatactaca 1921 aatccttgga gaaagcttac ccaactttct tgtacaaagt ggttggtaag cctatcccta 1981 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2041 agtcgacaat caacctctgg atta