Construct: ORF ccsbBroad304_07352

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011267.1_s304c1, BRDN0000400807
Derived from:
ccsbBroadEn_07352
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAPSS1 (9061)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07352
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... NM_005443.5 99.9% 100% 36A>G
2 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... XM_011532400.2 96.6% 96.6% 0_1ins63
3 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... XM_011532401.1 96.6% 96.6% 0_1ins63
4 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_011863.2 88% 98.7% (many diffs)
5 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289477.1 84.8% 95.3% (many diffs)
6 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289478.1 84.8% 95.3% (many diffs)
7 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289479.1 77.8% 87.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1938
ORF length:
1872
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gatccccggg agcctgtgca agaaagtcaa gctgagcaat aacgcgcaga
121actggggaat gcagagagca accaatgtca cctaccaagc ccatcatgtc agcaggaaca
181agagaggtca ggtggtgggg accagaggtg gctttcgtgg ttgcacagtt tggctaacag
241gcttgtctgg agcgggaaag actactgtga gcatggcctt ggaggagtac ctggtttgtc
301atggtattcc atgctacact ctggatggtg acaatattcg tcaaggtctc aataaaaatc
361ttggctttag tcctgaagac agagaagaga atgttcgacg catcgcagaa gttgctaaac
421tgtttgcaga tgctggctta gtgtgcatca caagtttcat atcaccttac actcaggatc
481gcaacaatgc aaggcaaatt catgaaggtg caagtttacc gttttttgaa gtatttgttg
541atgctcctct gcatgtttgt gaacagaggg atgtcaaagg actctacaaa aaagcccggg
601caggagaaat taaaggtttc actgggatcg attctgaata tgaaaagcca gaggcccctg
661agttggtgct gaaaacagac tcctgtgatg taaatgactg tgtccagcaa gttgtggaac
721ttctacagga acgggatatt gtacctgtgg atgcatctta tgaagtaaaa gaactatatg
781tgccagaaaa taaacttcat ttggcaaaaa cagatgcgga aacattacca gcactgaaaa
841ttaataaagt ggatatgcag tgggtgcagg ttttggcaga aggttgggca accccattga
901atggctttat gagagagagg gagtacttgc agtgccttca ttttgattgt cttctggatg
961gaggtgtcat taacttgtca gtacctatag ttctgactgc gactcatgaa gataaagaga
1021ggctggacgg ctgtacagca tttgctctga tgtatgaggg ccgccgtgtg gccattcttc
1081gcaatccaga gttttttgag cacaggaaag aggagcgctg tgccagacag tggggaacga
1141catgcaagaa ccacccctat attaagatgg tgatggaaca aggagattgg ctgattggag
1201gagatcttca agtcttggat cgagtttatt ggaatgatgg tcttgatcag tatcgtctta
1261ctcctactga gctaaagcag aaatttaaag atatgaatgc tgatgctgtc tttgcatttc
1321aactacgcaa cccagtgcac aatggacatg ccctgttaat gcaggatacc cataagcaac
1381ttctagagag gggctaccgg cgccctgtcc tcctcctcca ccctctgggt ggctggacaa
1441aggatgacga tgttcctttg atgtggcgta tgaagcagca tgctgcagtg ttggaggaag
1501gagttctgaa tcctgagacg acagtggtgg ccatcttccc atctcccatg atgtatgctg
1561gaccaactga ggtccagtgg cattgcagag cacggatggt tgcaggagcc aacttttaca
1621ttgttggacg agaccctgct ggcatgcctc atccagaaac agggaaggat ctttatgagc
1681caagtcatgg tgccaaagtg ctgacgatgg cccctggttt aatcactttg gaaatagttc
1741cctttcgagt tgcagcttac aacaagaaaa agaagcgtat ggactactat gactctgaac
1801accatgaaga ctttgaattt atttcaggaa cacgaatgcg caaacttgct cgagaaggcc
1861agaaaccacc tgaaggtttc atggctccca aggcttggac cgtgctgaca gaatactaca
1921aatccttgga gaaagcttac ccaactttct tgtacaaagt ggttggtaag cctatcccta
1981accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2041agtcgacaat caacctctgg atta

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