Construct: ORF ccsbBroad304_07354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000122.1_s304c1, BRDN0000400259
- Derived from:
- ccsbBroadEn_07354
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASH2L (9070)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07354
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | NM_001105214.2 | 99.9% | 100% | 972A>G |
| 2 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | XM_005273683.1 | 98.8% | 98.8% | 571_588del;990A>G |
| 3 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | NM_001261832.1 | 93.7% | 93.8% | 972A>G;1337_1338ins99 |
| 4 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | NM_001282272.1 | 91.5% | 91.5% | 0_1ins135;837A>G |
| 5 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | NM_004674.5 | 84.9% | 85% | 1_282del;1254A>G |
| 6 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | XM_005273682.1 | 84.1% | 84.2% | 1_282del;853_870del;1272A>G |
| 7 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | XM_006716413.3 | 79.7% | 79.7% | 1_282del;1254A>G;1619_1620ins99 |
| 8 | human | 9070 | ASH2L | ASH2 like, histone lysine m... | XM_006716412.1 | 78.9% | 79% | (many diffs) |
| 9 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | NM_001080793.2 | 90.1% | 99.2% | (many diffs) |
| 10 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | NM_001286207.1 | 90.1% | 99.2% | (many diffs) |
| 11 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | XM_011242136.1 | 89.1% | 98.1% | (many diffs) |
| 12 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | NM_011791.3 | 77.2% | 85% | (many diffs) |
| 13 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | XM_011242135.2 | 76.5% | 84.2% | (many diffs) |
| 14 | mouse | 23808 | Ash2l | ash2 (absent, small, or hom... | XR_001778438.1 | 56.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggaatgga tactcaggcg ggctccgtgg atgaagagaa tggccgacag ttgggtgagg 121 tagagctgca atgtgggatt tgtacaaaat ggttcacggc tgacacattt ggcatagata 181 cctcatcctg tctacctttc atgaccaact acagttttca ttgcaacgtc tgccatcaca 241 gtgggaatac ctatttcctc cggaagcaag caaacttgaa ggaaatgtgc cttagtgctt 301 tggccaacct gacatggcag tcccgaacac aggatgaaca tccgaagaca atgttctcca 361 aagataagga tattatacca tttattgata aatactggga gtgcatgaca accagacaga 421 gacctgggaa aatgacttgg ccaaataaca ttgttaaaac aatgagtaaa gaaagagatg 481 tattcttggt aaaggaacac ccagatccag gcagtaaaga tccagaagaa gattacccca 541 aatttggact tttggatcag gaccttagta acattggtcc tgcttatgac aaccaaaaac 601 agagcagtgc tgtgtctact agtgggaatt taaatggggg aattgcagca ggaagcagcg 661 gaaaaggacg aggagccaag cgcaaacagc aggatggagg gaccacaggg accaccaaga 721 aggcccggag tgaccctttg ttttctgctc agcgccttcc ccctcatggc tacccattgg 781 aacacccgtt taacaaagat ggctatcggt atattctagc tgagcctgat ccgcacgccc 841 ctgaccccga gaagctggaa cttgactgct gggcaggaaa acctattcct ggagacctct 901 acagagcctg cttgtatgaa cgggttttgt tagccctaca tgatcgagct ccccagttaa 961 agatctcaga tgaccggctg actgtggttg gagagaaggg ctactctatg gtgagggcct 1021 ctcatggagt acggaagggt gcctggtatt ttgaaatcac tgtggatgag atgccaccag 1081 ataccgctgc cagactgggt tggtcccagc ccctaggaaa ccttcaagct cctttaggtt 1141 atgataaatt tagctattct tggcggagca aaaagggaac caagttccac cagtccattg 1201 gcaaacacta ctcttctggc tatggacagg gagacgtcct gggattttat attaatcttc 1261 ctgaagacac agagacagcc aagtcattgc cagacacata caaagataag gctttgataa 1321 aattcaagag ttatttgtat tttgaggaaa aagactttgt ggataaagca gagaagagcc 1381 tgaagcagac tccccatagt gagataatat tttataaaaa tggtgtcaat caaggtgtgg 1441 cttacaaaga tatttttgag ggggtttact tcccagccat ctcactgtac aagagctgca 1501 cggtttccat taactttgga ccatgcttca agtatcctcc gaaggatctc acttaccgcc 1561 ctatgagtga catgggctgg ggcgccgtgg tagagcacac cctggctgac gtcttgtatc 1621 acgtggagac agaagtggat gggaggcgca gtcccccatg ggaaccctgc ccaactttct 1681 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1741 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta