Construct: ORF ccsbBroad304_07436

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009495.1_s304c1, BRDN0000406827
Derived from:
ccsbBroadEn_07436
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPTLC2 (9517)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07436
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9517 SPTLC2 serine palmitoyltransferase... NM_004863.3 99.9% 100% 786T>C
2 human 9517 SPTLC2 serine palmitoyltransferase... XM_011537384.2 79.6% 78% (many diffs)
3 mouse 20773 Sptlc2 serine palmitoyltransferase... NM_011479.4 90.6% 96% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1752
ORF length:
1686
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg gccggagccc ggaggctgct gctgccgccg cacggtgcgg gcgaatggct
121gcgtggcgaa cggggaagta cggaacgggt acgtgaggag cagcgctgca gccgcagccg
181cagccgccgc cggccagatc catcatgtta cacaaaatgg aggactatat aaaagaccgt
241ttaatgaagc ttttgaagaa acaccaatgc tggttgctgt gctcacgtat gtggggtatg
301gcgtactcac cctctttgga tatcttcgag atttcttgag gtattggaga attgaaaagt
361gtcaccatgc aacagaaaga gaagaacaaa aggactttgt gtcattgtat caagattttg
421aaaactttta tacaaggaat ctgtacatga ggataagaga caactggaat cggccaatct
481gtagtgtgcc tggagccagg gtggacatca tggagagaca gtctcatgat tataactggt
541ccttcaagta tacagggaat ataataaagg gtgttataaa catgggttcc tacaactatc
601ttggatttgc acggaatact ggatcatgtc aagaagcagc cgccaaagtc cttgaggagt
661atggagctgg agtgtgcagt actcggcagg aaattggaaa cctggacaag catgaagaac
721tagaggagct tgtagcaagg ttcttaggag tagaagctgc tatggcgtat ggcatgggat
781ttgcaacgaa ttcaatgaac attcctgctc ttgttggcaa aggttgcctg attctgagtg
841atgaactgaa ccatgcatca ctggttctgg gagccagact gtcaggagca accattagaa
901tcttcaaaca caacaatatg caaagcctag agaagctatt gaaagatgcc attgtttatg
961gtcagcctcg gacacgaagg ccctggaaga aaattctcat ccttgtggaa ggaatatata
1021gcatggaggg atctattgtt cgtcttcctg aagtgattgc cctcaagaag aaatacaagg
1081catacttgta tctggatgag gctcacagca ttggcgccct gggccccaca ggccggggtg
1141tggtggagta ctttggcctg gatcccgagg atgtggatgt tatgatggga acgttcacaa
1201agagttttgg tgcttctgga ggatatattg gaggcaagaa ggagctgata gactacctgc
1261gaacacattc tcatagtgca gtgtatgcca cgtcattgtc acctcctgta gtggagcaga
1321tcatcacctc catgaagtgc atcatggggc aggatggcac cagccttggt aaagagtgtg
1381tacaacagtt agctgaaaac accaggtatt tcaggagacg cctgaaagag atgggcttca
1441tcatctatgg aaatgaagac tctccagtag tgcctttgat gctctacatg cctgccaaaa
1501ttggcgcctt tggacgggag atgctgaagc ggaacatcgg tgtcgttgtg gttggatttc
1561ctgccacccc aattattgag tccagagcca ggttttgcct gtcagcagct cataccaaag
1621aaatacttga tactgcttta aaggagatag atgaagttgg ggacctattg cagctgaagt
1681attcccgtca tcggttggta cctctactgg acaggccctt tgacgagacg acgtatgaag
1741aaacagaaga ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1801tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1861caatcaacct ctggatta

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