Construct: ORF ccsbBroad304_07443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003976.1_s304c1, BRDN0000405943
- Derived from:
- ccsbBroadEn_07443
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1G1 (9550)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07443
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9550 | ATP6V1G1 | ATPase H+ transporting V1 s... | NM_004888.4 | 99.7% | 100% | 345C>T |
| 2 | mouse | 66290 | Atp6v1g1 | ATPase, H+ transporting, ly... | NM_024173.2 | 91.2% | 94.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tagtcagtct caggggattc agcagctgct gcaggccgag aagcgggcag 121 ccgagaaggt gtccgaggcc cgcaaaagaa agaaccggag gctgaagcag gccaaagaag 181 aagctcaggc tgaaattgaa cagtaccgcc tgcagaggga gaaagaattc aaggccaagg 241 aagctgcggc attgggatcc cgtggcagtt gcagcactga agtggagaag gagacccagg 301 agaagatgac catcctccag acatacttcc ggcagaacag ggatgaagtc ttggacaacc 361 tcttggcttt tgtctgtgac attcggccag aaatccatga aaactaccgt ataaatggat 421 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta