Construct: ORF ccsbBroad304_07454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000181.1_s304c1, BRDN0000403736
- Derived from:
- ccsbBroadEn_07454
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP155 (9631)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07454
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9631 | NUP155 | nucleoporin 155 | NM_153485.3 | 99.9% | 99.9% | 9T>N;201G>A |
| 2 | human | 9631 | NUP155 | nucleoporin 155 | NM_004298.4 | 95.7% | 95.7% | 0_1ins177;24G>A |
| 3 | human | 9631 | NUP155 | nucleoporin 155 | NM_001278312.2 | 95.3% | 95.3% | 9T>N;201G>A;2433_2434ins192 |
| 4 | human | 9631 | NUP155 | nucleoporin 155 | XR_001742368.2 | 79.7% | (many diffs) | |
| 5 | human | 9631 | NUP155 | nucleoporin 155 | XM_011514165.3 | 63.6% | 62.9% | (many diffs) |
| 6 | human | 9631 | NUP155 | nucleoporin 155 | XR_001742369.2 | 62.8% | (many diffs) | |
| 7 | mouse | 170762 | Nup155 | nucleoporin 155 | NM_133227.3 | 87.4% | 94.8% | (many diffs) |
| 8 | mouse | 170762 | Nup155 | nucleoporin 155 | XM_006519972.2 | 77.7% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4239
- ORF length:
- 4173
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtcntctttg ttgggcgcgg cgatgccggc ctctacatct gccgcagccc 121 tgcaggaagc tctggaaaat gctggacggc tcatcgaccg tcagttgcaa gaggaccgca 181 tgtacccgga cctttccgag ctgcttatgg tgtctgcccc aaataatccc accgtttctg 241 gcatgtcaga tatggattat cctttacaag gacctggttt gctgtccgta cccaaccttc 301 cagagatcag ttccatccga agagttcctc tcccacctga acttgttgag cagtttggac 361 atatgcagtg taattgcatg atgggtgtgt tccctcctat cagcagagct tggctcacaa 421 ttgacagtga tatattcatg tggaactatg aggatggagg agaccttgcc tattttgatg 481 gacttagtga gactattctt gctgtggggc ttgtgaagcc aaaagcaggc atctttcaac 541 ctcatgtgcg acacctcctg gttttggcga cccctgtaga catagtaatt cttggactca 601 gctatgctaa tttgcaaaca ggttctggag ttcttaatga tagtttgtct ggtggaatgc 661 agttgcttcc agatccttta tattctcttc ctactgataa tacttacctt ttaacaataa 721 cttccactga taatggcaga attttcttgg ctggaaagga tggctgttta tatgaagtag 781 cctaccaggc tgaagcaggg tggtttagcc aaagatgtag gaaaataaac cactcaaaga 841 gctcactttc tttccttgtt ccttccttgc tacaattcac gttctcagaa gatgatccta 901 ttcttcaaat tgcaattgat aattctagaa atattttata tacacgatct gagaaaggag 961 taatacaggt gtatgatttg ggacaagatg gacaaggaat gagcagagtt gcctctgtgt 1021 cacagaatgc cattgtctct gctgctggta acattgctag gaccatcgat cgttctgttt 1081 ttaaaccaat tgtccaaata gcagtgattg aaaattctga atcactggac tgtcagttat 1141 tggctgtcac acatgcaggt gttaggttat attttagcac ttgtccattc agacagccat 1201 tagcacggcc taatacactg acgctggttc atgtccgctt acctcctgga ttctcagcat 1261 cttcaaccgt ggaaaagcct tcaaaagtac atagagctct ttatagtaaa ggtattctat 1321 tgatggcagc ctcagaaaat gaggataatg atattttatg gtgtgtcaac catgatactt 1381 ttcctttcca aaagccaatg atggaaaccc agatgacagc tggtgttgat ggtcattcct 1441 gggctctttc tgcgatagat gaattgaaag tagataaaat aattacacct ttaaacaagg 1501 atcatattcc aataactgat tcaccagttg ttgtacagca gcacatgtta cctccgaaga 1561 aatttgttct cctctcagca caggggagcc ttatgtttca taaacttaga cctgtagatc 1621 aactgaggca tctacttgtg agtaatgtgg gaggagatgg agaagagatt gaaagattct 1681 ttaaattaca tcaggaagac caggcttgtg caacttgcct tattcttgct tgctccactg 1741 ctgcctgtga tagagaagta tctgcctggg ctactcgggc tttctttagg tatggtggtg 1801 aagcacagat gagatttcca accactcttc cgcctccaag taatgttggt cccatcttgg 1861 ggtctcctgt ctattctagt tctcctgttc ctagtggtag tccctatcca aatccatcct 1921 ttttgggaac accgtctcat ggtatacagc ctcctgccat gtcaactcca gtgtgtgctc 1981 tgggaaaccc agcaactcag gccacaaata tgagttgtgt gactggacca gagattgtgt 2041 actctggaaa acacaatggt atttgcattt acttttctcg gatcatggga aacatttggg 2101 atgcaagctt agttgtggag agaatattca agagtggcaa cagagagatc actgcaattg 2161 aaagtagtgt tccctgccaa ctgctagagt cagtgctaca agaactaaag ggtttgcagg 2221 aatttctaga cagaaactcc cagtttgcag gaggaccatt aggaaatcca aatactactg 2281 ctaaagtgca gcagaggctg ataggattca tgcgtcctga aaacggaaat ccccagcaaa 2341 tgcaacagga actgcagagg aagtttcatg aggctcaact aagtgaaaag atttcacttc 2401 aggcaattca gcagttggtt cgaaaatcat atcaggctct ggctttatgg aaacttcttt 2461 gtgaacatca attcactatc attgtggcag aacttcagaa ggaacttcaa gagcagctga 2521 agatcaccac ctttaaagat cttgtaatca gggacaaaga actcacaggg gcattaattg 2581 cttctcttat caactgctac atcagagata atgccgctgt tgatggcatt agtttacatt 2641 tacaggatat ctgcccactt ctatatagca ctgatgatgc aatttgttct aaggcaaatg 2701 agcttctcca gcgttcccga caagttcaaa ataagactga aaaagaaaga atgttaaggg 2761 aatcattaaa ggaatatcaa aaaattagca atcaagtgga cctttccaat gtttgtgctc 2821 agtatagaca agtgagattt tatgagggtg tggtggaact ttctcttacg gctgcagaga 2881 aaaaagatcc tcaaggtctt gggcttcatt tctataaaca tggagaacca gaagaagaca 2941 tagttggact tcaggccttc caagaaagat taaacagtta caaatgcatt acagacacac 3001 ttcaagaact ggtaaatcaa agtaaggccg ctcctcagtc tcccagtgta cccaaaaaac 3061 ctggtcctcc agtgttgtca tctgatccaa atatgctgag taatgaagaa gcaggacatc 3121 attttgaaca aatgcttaaa ttgtcacagc gatccaagga tgagctcttt agtattgccc 3181 tttataattg gctaatacaa gtcgaccttg cagataagct gctacaggtt gcttctccat 3241 ttctggagcc acatctagtc cgaatggcca aagttgatca aaacagagtt cgttatatgg 3301 atttactctg gcggtattac gagaagaaca gaagtttcag taatgctgct cgtgtactgt 3361 ccagactggc tgacatgcat agcacagaaa tttcacttca gcagcgacta gagtacattg 3421 ctcgagccat tcttagtgcc aaaagttcca ctgccatttc atcaatagct gccgatggtg 3481 aatttcttca tgaattagaa gaaaaaatgg aggttgctag gatccaactt cagatacagg 3541 agacactaca aaggcagtat tcccatcatt cttctgtaca ggatgcagtt tctcagctgg 3601 attctgagct gatggacata actaagcttt atggggaatt tgctgaccca tttaaacttg 3661 cagagtgcaa acttgcaata attcattgtg ccggttattc agaccctata ttggtgcaga 3721 cactttggca agatatcata gagaaagaat tgagtgacag tgtgacattg agctcctcgg 3781 atagaatgca tgctcttagt ctcaagattg ttctccttgg caaaatttat gctggcacac 3841 cacgcttctt tcctttagat tttattgtac agtttttaga acagcaggtt tgtactttga 3901 actgggatgt gggcttcgta atacagacaa tgaatgaaat tggagtacca ttacctagac 3961 tactagaagt ttatgatcag ttgttcaaat cacgggatcc attctggaac agaatgaaga 4021 agccactgca ccttttggat tgtatacatg tattattgat aagatatgtt gagaatccca 4081 gccaagtttt aaattgtgaa aggagaagat ttacaaatct ctgcctggat gctgtttgtg 4141 gttaccttgt tgagctccag tctatgagct cgtcagtagc agtacaagcc atcactggga 4201 attttaaatc tcttcaagct aaattagaac ggcttcatta cccaactttc ttgtacaaag 4261 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 4321 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta