Construct: ORF ccsbBroad304_07533
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003248.1_s304c1, BRDN0000401732
- Derived from:
- ccsbBroadEn_07533
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDCD6 (10016)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07533
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10016 | PDCD6 | programmed cell death 6 | NM_013232.4 | 99.8% | 99.4% | 438T>N |
| 2 | human | 10016 | PDCD6 | programmed cell death 6 | NM_001267556.2 | 98.7% | 98.4% | 360_361insGGTTTC;432T>N |
| 3 | human | 10016 | PDCD6 | programmed cell death 6 | NM_001267557.1 | 64.2% | 63.8% | 161_162ins204;234T>N |
| 4 | human | 10016 | PDCD6 | programmed cell death 6 | NM_001267558.1 | 63.1% | 62.8% | 0_1ins210;228T>N |
| 5 | human | 10016 | PDCD6 | programmed cell death 6 | NM_001267559.2 | 36.1% | 36.1% | 207_208ins366 |
| 6 | mouse | 18570 | Pdcd6 | programmed cell death 6 | NM_011051.3 | 86.7% | 98.4% | (many diffs) |
| 7 | mouse | 18570 | Pdcd6 | programmed cell death 6 | XM_006517156.1 | 85.8% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 642
- ORF length:
- 573
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgcctac tcttaccgcc ccggccctgg ggccggccct gggcctgctg 121 caggcgcggc gctgccggac cagagcttcc tgtggaacgt tttccagagg gtcgataaag 181 acaggagtgg agtgatatca gacaccgagc ttcagcaagc tctctccaac ggcacgtgga 241 ctccctttaa tccagtgact gtcaggtcga tcatatccat gtttgaccgt gagaacaagg 301 ccggcgtgaa cttcagcgag ttcacgggtg tgtggaagta catcacggac tggcagaacg 361 tcttccgcac gtacgaccgg gacaactccg ggatgatcga taagaacgag ctgaagcagg 421 ccctctcagg tttcggctac cggctctctg accagttcca cgacatcctc attcgaaagt 481 ttgacaggca gggacggggg cagatngcct tcgacgactt catccagggc tgcatcgtcc 541 tgcagaggtt gacggatata ttcagacgtt acgacacgga tcaggacggc tggattcagg 601 tgtcgtacga acagtacctg tccatggtct tcagtatcgt attgccaact ttcttgtaca 661 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 721 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta