Construct: ORF ccsbBroad304_07557

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000597.1_s304c1, BRDN0000408012
Derived from:
ccsbBroadEn_07557
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NAMPT (10135)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07557
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10135 NAMPT nicotinamide phosphoribosyl... NM_005746.3 99.9% 100% 903A>G
2 mouse 59027 Nampt nicotinamide phosphoribosyl... NM_021524.2 91.4% 95.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1539
ORF length:
1473
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tcctgcggca gaagccgagt tcaacatcct cctggccacc gactcctaca
121aggttactca ctataaacaa tatccaccca acacaagcaa agtttattcc tactttgaat
181gccgtgaaaa gaagacagaa aactccaaat taaggaaggt gaaatatgag gaaacagtat
241tttatgggtt gcagtacatt cttaataagt acttaaaagg taaagtagta accaaagaga
301aaatccagga agccaaagat gtctacaaag aacatttcca agatgatgtc tttaatgaaa
361agggatggaa ctacattctt gagaagtatg atgggcatct tccaatagaa ataaaagctg
421ttcctgaggg ctttgtcatt cccagaggaa atgttctctt cacggtggaa aacacagatc
481cagagtgtta ctggcttaca aattggattg agactattct tgttcagtcc tggtatccaa
541tcacagtggc cacaaattct agagagcaga agaaaatatt ggccaaatat ttgttagaaa
601cttctggtaa cttagatggt ctggaataca agttacatga ttttggctac agaggagtct
661cttcccaaga gactgctggc ataggagcat ctgctcactt ggttaacttc aaaggaacag
721atacagtagc aggacttgct ctaattaaaa aatattatgg aacgaaagat cctgttccag
781gctattctgt tccagcagca gaacacagta ccataacagc ttgggggaaa gaccatgaaa
841aagatgcttt tgaacatatt gtaacacagt tttcatcagt gcctgtatct gtggtcagcg
901atagctatga catttataat gcgtgtgaga aaatatgggg tgaagatcta agacatttaa
961tagtatcgag aagtacacag gcaccactaa taatcagacc tgattctgga aaccctcttg
1021acactgtgtt aaaggttttg gagattttag gtaagaagtt tcctgttact gagaactcaa
1081agggttacaa gttgctgcca ccttatctta gagttattca aggggatgga gtagatatta
1141ataccttaca agagattgta gaaggcatga aacaaaaaat gtggagtatt gaaaatattg
1201ccttcggttc tggtggaggt ttgctacaga agttgacaag agatctcttg aattgttcct
1261tcaagtgtag ctatgttgta actaatggcc ttgggattaa cgtcttcaag gacccagttg
1321ctgatcccaa caaaaggtcc aaaaagggcc gattatcttt acataggacg ccagcaggga
1381attttgttac actggaggaa ggaaaaggag accttgagga atatggtcag gatcttctcc
1441atactgtctt caagaatggc aaggtgacaa aaagctattc atttgatgaa ataagaaaaa
1501atgcacagct gaatattgaa ctggaagcag cacatcatta cccaactttc ttgtacaaag
1561tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1621gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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