Transcript: Mouse NM_021524.2

Mus musculus nicotinamide phosphoribosyltransferase (Nampt), mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Nampt (59027)
Length:
4547
CDS:
236..1711

Additional Resources:

NCBI RefSeq record:
NM_021524.2
NBCI Gene record:
Nampt (59027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_021524.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306267 TACAAGGTTACTCACTATAAA pLKO_005 287 CDS 100% 15.000 21.000 N Nampt n/a
2 TRCN0000306327 TCAGCGATAGCTATGACATTT pLKO_005 1065 CDS 100% 13.200 18.480 N Nampt n/a
3 TRCN0000306266 TTCAGTCCTGGTATCCAATTA pLKO_005 693 CDS 100% 13.200 18.480 N Nampt n/a
4 TRCN0000101278 CCTACTTTGAATGCCGTGAAA pLKO.1 339 CDS 100% 4.950 6.930 N Nampt n/a
5 TRCN0000101277 GCCACCTTATCTTAGAGTCAT pLKO.1 1267 CDS 100% 4.950 6.930 N Nampt n/a
6 TRCN0000101275 GCCAGATTATTTGTTGGTTTA pLKO.1 1840 3UTR 100% 10.800 8.640 N Nampt n/a
7 TRCN0000424413 AGCGATAGCTATGACATTTAT pLKO_005 1067 CDS 100% 15.000 10.500 N NAMPT n/a
8 TRCN0000311503 AGATGTGTGGGTTCGTGTTTA pLKO_005 1807 3UTR 100% 13.200 9.240 N Nampt n/a
9 TRCN0000101279 GCGCTTTGCTACAGAAGTTAA pLKO.1 1386 CDS 100% 13.200 9.240 N Nampt n/a
10 TRCN0000101276 CCACCTTATCTTAGAGTCATT pLKO.1 1268 CDS 100% 4.950 3.465 N Nampt n/a
11 TRCN0000326592 CCACCTTATCTTAGAGTCATT pLKO_005 1268 CDS 100% 4.950 3.465 N Nampt n/a
12 TRCN0000116180 GTAACTTAGATGGTCTGGAAT pLKO.1 777 CDS 100% 4.950 3.465 N NAMPT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021524.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07557 pDONR223 100% 91.4% 95.7% None (many diffs) n/a
2 ccsbBroad304_07557 pLX_304 0% 91.4% 95.7% V5 (many diffs) n/a
3 TRCN0000467417 CGTAGTAACGCTTTCGGATACGAT pLX_317 27.1% 91.4% 95.7% V5 (many diffs) n/a
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