Construct: ORF ccsbBroad304_07575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006599.1_s304c1, BRDN0000399970
- Derived from:
- ccsbBroadEn_07575
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS31 (10240)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07575
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10240 | MRPS31 | mitochondrial ribosomal pro... | NM_005830.4 | 99.8% | 99.4% | 239C>T;394A>G |
| 2 | human | 10240 | MRPS31 | mitochondrial ribosomal pro... | XR_001749454.2 | 83.1% | (many diffs) | |
| 3 | human | 10240 | MRPS31 | mitochondrial ribosomal pro... | XR_001749453.2 | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcctagagtc tcgacgttcc tacctcttcg ccccctttcc cgccaccctt 121 tgtcctctgg aagcccggag acatcagcgg ctgcgattat gctactcact gttcggcacg 181 gaacagtcag gtaccgcagt tcagcgctgt tggcccggac aaaaaataac atccaaagat 241 attttggcac taacagtgtg atctgtagca agaaagataa gcagtctgtt cgaactgagg 301 agatttccaa ggagacttca gagagccaag acagtgaaaa ggaaaatacg aaaaaagact 361 tgttaggcat tattaagggc atgaaagttg aattaagcac agtaaatgta cgaacaacaa 421 agccccccaa aagaagacca cttaaaagtt tggaagctgc acttggcagg cttcgaagag 481 ctacagaata tgctccaaag aagagaattg agcccctgag tcctgagttg gtggcagctg 541 catctgctgt ggcagattct ctcccttttg ataagcaaac aaccaagtca gagctgctga 601 gccagctcca gcagcatgag gaagagtcaa gggcacagag agatgcaaag cgacctaaaa 661 ttagtttcag taacataata tcagatatga aagttgccag atctgctaca gctagagttc 721 gttcaagacc agagcttcgg attcagtttg atgaaggcta tgacaattat cctggccagg 781 agaagacgga tgatcttaaa aaaaggaaaa atatattcac agggaaaaga cttaatattt 841 ttgacatgat ggcagttact aaagaagcac ctgaaacaga cacatcacct tcactttggg 901 atgtggaatt tgctaagcag ttagccacag taaatgaaca accccttcag aatggatttg 961 aagagctgat ccagtggaca aaagagggga aactatggga gttcccaatt aacaatgaag 1021 caggttttga tgatgatggt tcagaatttc atgaacatat atttctggag aaacacctgg 1081 agagctttcc aaaacaagga ccaattcgcc acttcatgga gctggtgact tgtggccttt 1141 ccaaaaaccc atatcttagt gttaaacaga aggttgaaca catagagtgg tttagaaatt 1201 attttaatga aaaaaaggat attctaaaag aaagtaacat acagttcaat tacccaactt 1261 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1321 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta