Construct: ORF ccsbBroad304_07611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015328.1_s304c1, BRDN0000406845
- Derived from:
- ccsbBroadEn_07611
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPNMB (10457)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07611
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10457 | GPNMB | glycoprotein nmb | NM_001005340.2 | 99.8% | 99.8% | 1497T>C;1712T>C |
| 2 | human | 10457 | GPNMB | glycoprotein nmb | NM_002510.3 | 97.7% | 97.7% | 1016_1017ins36;1461T>C;1676T>C |
| 3 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011676.2 | 95.2% | 95.1% | 1017_1100del;1581T>C;1796T>C |
| 4 | human | 10457 | GPNMB | glycoprotein nmb | XM_005249578.3 | 91.5% | 90.9% | 1497T>C;1560_1570del;1593_1594ins134 |
| 5 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011678.2 | 89.4% | 88.8% | (many diffs) |
| 6 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011677.2 | 87.2% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga atgtctctac tatttcctgg gatttctgct cctggctgca agattgccac 121 ttgatgccgc caaacgattt catgatgtgc tgggcaatga aagaccttct gcttacatga 181 gggagcacaa tcaattaaat ggctggtctt ctgatgaaaa tgactggaat gaaaaactct 241 acccagtgtg gaagcgggga gacatgaggt ggaaaaactc ctggaaggga ggccgtgtgc 301 aggcggtcct gaccagtgac tcaccagccc tcgtgggctc aaatataaca tttgcggtga 361 acctgatatt ccctagatgc caaaaggaag atgccaatgg caacatagtc tatgagaaga 421 actgcagaaa tgaggctggt ttatctgctg atccgtatgt ttacaactgg acagcatggt 481 cagaggacag tgacggggaa aatggcaccg gccaaagcca tcataacgtc ttccctgatg 541 ggaaaccttt tcctcaccac cccggatgga gaagatggaa tttcatctac gtcttccaca 601 cacttggtca gtatttccag aaattgggac gatgttcagt gagagtttct gtgaacacag 661 ccaatgtgac acttgggcct caactcatgg aagtgactgt ctacagaaga catggacggg 721 catatgttcc catcgcacaa gtgaaagatg tgtacgtggt aacagatcag attcctgtgt 781 ttgtgactat gttccagaag aacgatcgaa attcatccga cgaaaccttc ctcaaagatc 841 tccccattat gtttgatgtc ctgattcatg atcctagcca cttcctcaat tattctacca 901 ttaactacaa gtggagcttc ggggataata ctggcctgtt tgtttccacc aatcatactg 961 tgaatcacac gtatgtgctc aatggaacct tcagccttaa cctcactgtg aaagctgcag 1021 caccaggacc ttgtccgcca ccgccaccac cacccagacc ttcaaaaccc accccttctt 1081 tagcaactac tctaaaatct tatgattcaa acaccccagg acctgctggt gacaaccccc 1141 tggagctgag taggattcct gatgaaaact gccagattaa cagatatggc cactttcaag 1201 ccaccatcac aattgtagag ggaatcttag aggttaacat catccagatg acagacgtcc 1261 tgatgccggt gccatggcct gaaagctccc taatagactt tgtcgtgacc tgccaaggga 1321 gcattcccac ggaggtctgt accatcattt ctgaccccac ctgcgagatc acccagaaca 1381 cagtctgcag ccctgtggat gtggatgaga tgtgtctgct gactgtgaga cgaaccttca 1441 atgggtctgg gacgtactgt gtgaacctca ccctggggga tgacacaagc ctggctctca 1501 cgagcaccct gatttctgtt cctgacagag acccagcctc gcctttaagg atggcaaaca 1561 gcgccctgat ctccgttggc tgcttggcca tatttgtcac tgtgatctcc ctcttggtgt 1621 acaaaaaaca caaggaatac aacccaatag aaaatagtcc tgggaatgtg gtcagaagca 1681 aaggcctgag tgtctttctc aaccgtgcaa aagccgtgtt cttcccggga aaccaggaaa 1741 aggatccgct actcaaaaac caagaattta aaggagcttc ttacccaact ttcttgtaca 1801 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1861 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta