Construct: ORF ccsbBroad304_07623
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005915.1_s304c1, BRDN0000408320
- Derived from:
- ccsbBroadEn_07623
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4B (10509)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07623
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10509 | SEMA4B | semaphorin 4B | NM_020210.4 | 99.8% | 99.8% | 1588A>C;2181G>A;2389T>G |
| 2 | human | 10509 | SEMA4B | semaphorin 4B | NM_198925.3 | 99.8% | 99.8% | 1588A>C;2181G>A;2389T>G |
| 3 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324034.2 | 98.7% | 98.7% | (many diffs) |
| 4 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324031.2 | 93.9% | 93.9% | (many diffs) |
| 5 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324029.2 | 83.3% | 63.5% | (many diffs) |
| 6 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324030.2 | 81.2% | 81.2% | (many diffs) |
| 7 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324033.2 | 81.2% | 81.2% | (many diffs) |
| 8 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324032.2 | 70.5% | 66.5% | 1588A>C;1686_1704del;1806_1807ins724 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2580
- ORF length:
- 2511
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgcgcacc gcgatgggcc tgaggagctg gctcgccgcc ccatggggcg 121 cgctgccgcc tcggccaccg ctgctgctgc tcctgctgct gctgctcctg ctgcagccgc 181 cgcctccgac ctgggcgctc agcccccgga tcagcctgcc tctgggctct gaagagcggc 241 cattcctcag attcgaagct gaacacatct ccaactacac agcccttctg ctgagcaggg 301 atggcaggac cctgtacgtg ggtgctcgag aggccctctt tgcactcagt agcaacctca 361 gcttcctgcc aggcggggag taccaggagc tgctttgggg tgcagacgca gagaagaaac 421 agcagtgcag cttcaagggc aaggacccac agcgcgactg tcaaaactac atcaagatcc 481 tcctgccgct cagcggcagt cacctgttca cctgtggcac agcagccttc agccccatgt 541 gtacctacat caacatggag aacttcaccc tggcaaggga cgagaagggg aatgtcctcc 601 tggaagatgg caagggccgt tgtcccttcg acccgaattt caagtccact gccctggtgg 661 ttgatggcga gctctacact ggaacagtca gcagcttcca agggaatgac ccggccatct 721 cgcggagcca aagccttcgc cccaccaaga ccgagagctc cctcaactgg ctgcaagacc 781 cagcttttgt ggcctcagcc tacattcctg agagcctggg cagcttgcaa ggcgatgatg 841 acaagatcta ctttttcttc agcgagactg gccaggaatt tgagttcttt gagaacacca 901 ttgtgtcccg cattgcccgc atctgcaagg gcgatgaggg tggagagcgg gtgctacagc 961 agcgctggac ctccttcctc aaggcccagc tgctgtgctc acggcccgac gatggcttcc 1021 ccttcaacgt gctgcaggat gtcttcacgc tgagccccag cccccaggac tggcgtgaca 1081 cccttttcta tggggtcttc acttcccagt ggcacagggg aactacagaa ggctctgccg 1141 tctgtgtctt cacaatgaag gatgtgcaga gagtcttcag cggcctctac aaggaggtga 1201 accgtgagac acagcagtgg tacaccgtga cccacccggt gcccacaccc cggcctggag 1261 cgtgcatcac caacagtgcc cgggaaagga agatcaactc atccctgcag ctcccagacc 1321 gcgtgctgaa cttcctcaag gaccacttcc tgatggacgg gcaggtccga agccgcatgc 1381 tgctgctgca gccccaggct cgctaccagc gcgtggctgt acaccgcgtc cctggcctgc 1441 accacaccta cgatgtcctc ttcctgggca ctggtgacgg ccggctccac aaggcagtga 1501 gcgtgggccc ccgggtgcac atcattgagg agctgcagat cttctcatcg ggacagcccg 1561 tgcagaatct gctcctggac acccacaggg ggctgctgta tgcggcctca cactcgggcg 1621 tagtccaggt gcccatggcc aactgcagcc tgtaccggag ctgtggggac tgcctcctcg 1681 cccgggaccc ctactgtgct tggagcggct ccagctgcaa gcacgtcagc ctctaccagc 1741 ctcagctggc caccaggccg tggatccagg acatcgaggg agccagcgcc aaggaccttt 1801 gcagcgcgtc ttcggttgtg tccccgtctt ttgtaccaac aggggagaag ccatgtgagc 1861 aagtccagtt ccagcccaac acagtgaaca ctttggcctg cccgctcctc tccaacctgg 1921 cgacccgact ctggctacgc aacggggccc ccgtcaatgc ctcggcctcc tgccacgtgc 1981 tacccactgg ggacctgctg ctggtgggca cccaacagct gggggagttc cagtgctggt 2041 cactagagga gggcttccag cagctggtag ccagctactg cccagaggtg gtggaggacg 2101 gggtggcaga ccaaacagat gagggtggca gtgtacccgt cattatcagc acatcgcgtg 2161 tgagtgcacc agctggtggc aaggccagct ggggtgcaga caggtcctac tggaaggagt 2221 tcctggtgat gtgcacgctc tttgtgctag ccgtgctgct cccagtttta ttcttgctct 2281 accggcaccg gaacagcatg aaagtcttcc tgaagcaggg ggaatgtgcc agcgtgcacc 2341 ccaagacctg ccctgtggtg ctgccccctg agacccgccc actcaacggc ctagggcccc 2401 ctagcacccc gctcgatcac cgagggtacc agtccctgtc agacagcccc ccgggggccc 2461 gagtcttcac tgagtcagag aagaggccac tcagcatcca agacagcttc gtggaggtat 2521 ccccagtgtg cccccggccc cgggtccgcc ttggctcgga gatccgtgac tctgtggtgt 2581 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2641 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2701 ggatta