Construct: ORF ccsbBroad304_07737
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014865.1_s304c1, BRDN0000407989
- Derived from:
- ccsbBroadEn_07737
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX52 (11056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07737
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11056 | DDX52 | DExD-box helicase 52 | NM_007010.5 | 99.9% | 100% | 792A>G |
| 2 | human | 11056 | DDX52 | DExD-box helicase 52 | XM_011524232.2 | 96.9% | 96.9% | 792A>G;1743_1744ins54 |
| 3 | human | 11056 | DDX52 | DExD-box helicase 52 | NM_001291476.2 | 81.9% | 81.9% | 0_1ins324;468A>G |
| 4 | human | 11056 | DDX52 | DExD-box helicase 52 | XM_011524233.3 | 59.4% | 58.5% | (many diffs) |
| 5 | mouse | 78394 | Ddx52 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_030096.2 | 86% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1863
- ORF length:
- 1797
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgtccacgat ctctttcgcc ggctcggcgc gggggccaaa ttcgacacga 121 gacgcttctc ggcagacgca gctcgattcc agataggaaa aaggaaatat gactttgatt 181 cttcggaggt gcttcaggga ctggactttt ttggaaacaa gaagtctgtc ccaggtgtgt 241 gtggagcatc acaaacacat cagaagcccc aaaatggaga gaaaaaagaa gagagcctaa 301 ctgaaaggaa gagggagcag agcaagaaaa aaaggaagac gatgacttca gaaattgctt 361 cccaagaaga aggtgctact atacagtgga tgtcatctgt agaagcaaag attgaagaca 421 aaaaagttca gagagaaagt aaactaactt ccggaaagtt ggagaatctc agaaaagaaa 481 agataaactt cttgcggaat aaacacaaaa ttcacgtcca aggaaccgat cttcctgacc 541 caattgctac atttcagcaa cttgaccagg aatataaaat caattctcga ctacttcaga 601 acattctaga tgcaggtttc caaatgccta cgccaatcca aatgcaagcc atcccagtta 661 tgctgcatgg tcgggaactt ctggcttctg ctccaactgg atctggaaaa acattagctt 721 ttagcattcc tattttaatg cagctgaaac aacccgcaaa taaaggcttc agagccctga 781 ttatatcacc aacacgagaa cttgccagcc agattcacag agagttaata aaaatttctg 841 agggaacagg attcaggata cacatgatcc acaaagcagc agtggcagcc aagaaatttg 901 gacctaaatc atctaaaaag tttgatattc ttgtgactac tccaaatcga ctaatctatt 961 tattaaagca agatcccccc ggaatcgacc tagcaagtgt tgagtggctt gtagtagacg 1021 aatcagataa actgtttgaa gatggcaaaa ctgggttcag agaccagctg gcttccattt 1081 tcctggcctg cacatcccac aaggtccgaa gagctatgtt cagtgcaact tttgcatatg 1141 atgttgaaca gtggtgcaaa ctcaacctgg acaatgtcat cagtgtgtcc attggagcaa 1201 ggaattctgc agtagaaact gtagaacaag agcttctctt tgttggatct gagaccggaa 1261 aacttctggc cgtgagagaa cttgttaaaa agggtttcaa tccacctgtt cttgtttttg 1321 ttcagtccat tgaaagggct aaagaacttt ttcatgagct catatatgaa ggtattaatg 1381 tggatgttat tcatgcagag agaacacaac aacagagaga taacacagtc cacagtttca 1441 gagcaggaaa aatctgggtt ctgatttgta cagccttgct agcaagaggg attgatttta 1501 aaggtgtgaa cttggtgatc aactatgact ttccaactag ctcagtggaa tatatccaca 1561 ggataggtcg aactggaaga gcagggaata agggaaaagc aattacattt ttcactgagg 1621 atgataagcc attattaaga agcgttgcta atgttataca gcaggctggg tgtcctgtac 1681 cagaatacat aaaaggtttt cagaaactac taagcaaaca aaagaaaaag atgattaaga 1741 aaccattgga aagggagagc attagtacaa ctccaaaatg tttcttagaa aaagctaagg 1801 ataaacagaa aaaggtcact ggtcagaaca gcaagaagaa agtagctctt gaagacaaaa 1861 gttacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1921 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1981 tctggatta