Construct: ORF ccsbBroad304_07741

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000640.1_s304c1, BRDN0000407215
Derived from:
ccsbBroadEn_07741
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAPGEF4 (11069)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07741
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11069 RAPGEF4 Rap guanine nucleotide exch... NM_001100397.1 99.8% 99.5% (many diffs)
2 human 11069 RAPGEF4 Rap guanine nucleotide exch... NM_001282899.1 98.7% 98.5% (many diffs)
3 human 11069 RAPGEF4 Rap guanine nucleotide exch... XM_017003197.1 97.7% 97.4% (many diffs)
4 human 11069 RAPGEF4 Rap guanine nucleotide exch... NM_001282900.1 96.6% 96.4% (many diffs)
5 human 11069 RAPGEF4 Rap guanine nucleotide exch... XM_006712205.3 96.6% 96.4% (many diffs)
6 human 11069 RAPGEF4 Rap guanine nucleotide exch... XM_011510505.3 96.5% 91.6% (many diffs)
7 human 11069 RAPGEF4 Rap guanine nucleotide exch... NM_001282901.1 91% 90.7% (many diffs)
8 human 11069 RAPGEF4 Rap guanine nucleotide exch... NM_007023.4 85.4% 85% (many diffs)
9 human 11069 RAPGEF4 Rap guanine nucleotide exch... XM_005246246.3 83.6% 83.2% (many diffs)
10 human 11069 RAPGEF4 Rap guanine nucleotide exch... XM_017003196.2 80% 79.2% (many diffs)
11 mouse 56508 Rapgef4 Rap guanine nucleotide exch... NM_001204166.1 91.5% 96.7% (many diffs)
12 mouse 56508 Rapgef4 Rap guanine nucleotide exch... XM_017319138.1 89.5% 94.8% (many diffs)
13 mouse 56508 Rapgef4 Rap guanine nucleotide exch... XM_017319139.1 88.6% 93.8% (many diffs)
14 mouse 56508 Rapgef4 Rap guanine nucleotide exch... XM_011239711.2 88.2% 92.7% (many diffs)
15 mouse 56508 Rapgef4 Rap guanine nucleotide exch... XM_006499938.3 83.2% 88.3% (many diffs)
16 mouse 56508 Rapgef4 Rap guanine nucleotide exch... NM_001204165.1 78.4% 82.7% (many diffs)
17 mouse 56508 Rapgef4 Rap guanine nucleotide exch... NM_019688.2 76.7% 81.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2667
ORF length:
2601
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ctacaagaaa tatcgacagt atatggcagg acttctggct cctccttatg
121gtgttatgga aacgggctct aacaatgaca ggattcctga caaggagaac acacctctca
181ttgaacctca cgttcctctt cgtcctgcta acaccattac caaggtccct tcagagaaga
241tcctcagagc tggaaaaatt ttacgaaatg ccattctctc tcgagcacct cacatgataa
301gagatagaaa ataccaccta aagacataca gacaatgctg tgtgggaact gaactggtgg
361actggatgat gcagcagaca ccatgtgttc actcccggac tcaagctgtt ggcatgtggc
421aagtcctgtt agaagatggt gttctcaacc acgtggacca ggagcaccat ttccaagaca
481aatatttatt ctatcgattt ctggatgatg agcacgagga tgcccctttg cctactgagg
541aggagaagaa ggagtgtgat gaggagctcc aggacaccat gctgctgctg tcacagatgg
601gccgcgacgc ccacatgagg atgatccttc gcaaaccacc tggccagagg actgtggatg
661acctagagat tatctatgag gagcttcttc atattaaagc cttatcccat ctttctacca
721cagtgaaacg agagttagca ggtgttctca tttttgagtc tcacgccaaa ggagggactg
781tgttgtttaa ccagggggaa gaaggtacct cctggtacat tattctaaaa ggatcagtga
841atgtagtcat ttacggcaag ggtgtggtct gcaccctgca tgaaggagat gacttcggca
901agttagcact agtgaatgat gccccacgag ctgcctctat cgtcttacga gaagataact
961gccatttctt aagagtagac aaggaggatt tcaaccggat cctaagggac gtggaggcga
1021atacagtcag acttaaagaa catgaccaag atgtcttggt gctggagaag gtcccagcag
1081ggaacagagc ttctaatcaa ggaaactcac agcctcagca aaagtatact gtgatgtcag
1141gaacacctga aaaaatttta gagcgttttc tagaaacaat acgccttgag gcaactttaa
1201atgaagcaac agattctgtt ttaaatgact ttattatgat gcactgtgtt tttatgccaa
1261atacccagct ttgcccggca ctggtggccc actaccacgc acagccttca caaggtacag
1321aacaggagaa aatggattat gccctcaaca ataagaggcg agtcatccgc ctggttctac
1381agtgggctgc catgtatgga gacctcctgc aagaggatga cgtgtctatg gccttcctgg
1441aggagtttta tgtatctgta tcagatgatg cccggatgat tgctgccctc aaggagcaac
1501tgccagagtt ggagaagatt gtcaagcaaa tctcagaaga tgcaaaggca ccacaaaaga
1561agcacaaggt tattttgcaa cagttcaata cgggcgatga gagagcccag aagcgccagc
1621ctatccgcgg ctctgatgaa gttctgttta aggtctattg catggaccac acctacacaa
1681ccattcgggt gccagtggcc acttcggtga aggaagtcat cagtgcagtt gccgacaagc
1741tgggctccgg ggagggcctg atcatagtca agatgagttc cggaggagaa aaggtggtgc
1801tcaaacctaa tgatgtttca gtatttacga cgctcaccat taatggacgc ctgtttgctt
1861gcccgcgaga gcaattcgat tcactgactc cattaccaga acaggaaggc ccaactgttg
1921gaacagtggg aacttttgaa ctgatgagct ccaaagattt agcataccag atgacaattt
1981atgattggga actcttcaac tgcgtgcatg agctggagct aatctatcac acatttggaa
2041ggcataattt taaaaagacc acagcaaact tggatttgtt cctgaggaga tttaatgaaa
2101ttcagttttg ggtcgtcact gagatctgcc tttgttctca gctcagcaag cgtgttcagc
2161tattaaaaaa atttattaag atagcagccc actgtaagga gtataaaaat ctgaattcct
2221tttttgccat cgtcatggga ctaagtaacg ttgctgtgag ccgcttggca ctaacgtggg
2281agaaactgcc aagcaagttc aagaagttct atgcggagtt tgaaagttta atggacactt
2341caaggaacca cagggcctac aggctgacag tagctaagct ggaacctcct ctcatcccct
2401tcatgccttt gctcattaaa gatatgacat ttactcatga ggggaacaag acgttcattg
2461acaatctagt aaactttgaa aaaatgcgca tgattgcaaa tacggccaga acagtgagat
2521actacaggag ccaacccttc aatcctgatg cagctcaagc taataagaac catcaggatg
2581tccggagtta tgtacggcaa ttaaatgtga ttgacaacca gagaacttta tcacagatgt
2641cacacagatt agagcctcgt cgaccatacc caactttctt gtacaaagtg gttggtaagc
2701ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2761ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.