Construct: ORF ccsbBroad304_07763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001136.1_s304c1, BRDN0000399531
- Derived from:
- ccsbBroadEn_07763
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT6 (11162)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07763
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11162 | NUDT6 | nudix hydrolase 6 | NM_007083.5 | 99.8% | 100% | 12A>G |
| 2 | human | 11162 | NUDT6 | nudix hydrolase 6 | NM_198041.3 | 46.5% | 46.5% | 0_1ins507 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcagccgctg agctggggcc gctggcgcgc gatgcttgcc cgaacctacg 121 gccccgggcc ttcggcgggt taccgctggg cctcgggcgc acagggttac gtgcggaatc 181 cgccagttgg agcgtgcgat ctgcagggcg agctggacag attcgggggc atctcggtgc 241 gcctggcgcg gctcgatgcg ctggaccgcc tggacgctgc cgccttccag aagggcttgc 301 aggctgcagt acagcaatgg cgatcagaag gtagaacagc tgtatggctg cacattccca 361 tcctccaaag ccgatttatt gcccctgctg cttccctggg cttctgcttt caccacgcag 421 aatcggattc atcaacgttg actctgtggc tgagagaagg gcccagcaga ttaccaggat 481 atgcttcaca tcaagtagga gttgcaggag ctgtatttga tgaaagtact agaaaaatac 541 tggttgtaca agatcgaaat aaattgaaaa atatgtggaa gtttccagga ggcctgtcag 601 agcctgaaga agatattgga gacacagcgg ttcgagaagt ttttgaagag actggtataa 661 aatcagaatt caggtccgtc ctgagtattc ggcaacagca cacaaatcct ggagcttttg 721 ggaagtcaga tatgtatatc atctgccgcc taaagccata ttcattcacc ataaattttt 781 gccaggaaga atgcttaaga tgtgagtgga tggatctcaa tgacctggcg aagactgaaa 841 atacaactcc catcaccagc agagttgcta ggctgctgct gtatgggtac agagaagggt 901 ttgacaaaat tgacctgact gtggaagaac ttccagcagt ttacacagga ctgttttata 961 aactctatca taaggaactg ccagagaatt ataaaactat gaaaggaatt gattacccaa 1021 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1081 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta