Construct: ORF ccsbBroad304_07782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011797.1_s304c1, BRDN0000411533
- Derived from:
- ccsbBroadEn_07782
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF139 (11236)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07782
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11236 | RNF139 | ring finger protein 139 | NM_007218.3 | 99.9% | 100% | 657C>T |
| 2 | mouse | 75841 | Rnf139 | ring finger protein 139 | NM_175226.4 | 91.1% | 93.1% | (many diffs) |
| 3 | mouse | 75841 | Rnf139 | ring finger protein 139 | XM_017316787.1 | 79.9% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2058
- ORF length:
- 1992
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgtgggg cccccgcagc agcaggtgcg gatggcccat cagcaggtct 121 gggcggcgct cgaagtggcg ctccgggtgc cctgccttta catcatcgac gccatcttca 181 actcctaccc ggattccagc caaagccggt tctgcatcgt gctccagatc ttcctccggc 241 tctttggtgt atttgcatcc agtattgttc tgatcttgtc acaacgatca cttttcaagt 301 tttacacgta cagctcagcc tttctgttag ctgcaacttc agtgttggtg aattattatg 361 cttctttgca cattgacttc tatggtgcct acaacacgtc agcttttgga attgagctgc 421 ttcctcgaaa aggtccctcg ctgtggatgg cacttatcgt tctacagcta acatttggaa 481 ttggatacgt tacactactc cagattcatt ccatctattc acaattaatt attttggatc 541 tcttggttcc tgtaataggc ttaatcacag agctaccatt acacatcaga gagactttac 601 tgtttacttc ttccttgatt ctcacattaa atacagtgtt tgtcctggca gtgaaactga 661 agtggtttta ttattccaca cgatatgttt atcttttggt gaggcacatg tatcgaattt 721 atggattaca gttattgatg gaggacacat ggaagaggat tcgtttccca gacatactac 781 gagtcttttg gctaacaaga gttacagctc aggctacagt gttaatgtac atcttaagga 841 tggcaaatga aactgattcc ttctttattt cttgggatga tttttgggac ctcatttgca 901 atcttataat tagtgggtgc gattctacac taactgtact gggcatgagt gctgtaattt 961 cctcagtagc ccattatttg gggcttggaa tattggcctt tattggatca actgaggaag 1021 atgacaggcg tcttggcttt gttgcacctg ttttattttt tattttggct cttcagactg 1081 ggttaagtgg gctaagacca gaagagagac ttattcgctt aagtagaaac atgtgccttt 1141 tattaactgc agtcctgcat tttatccatg gaatgacaga ccctgtatta atgtctctca 1201 gtgcctctca tgtgtcatct tttcgtagac attttcctgt gctgtttgtc tctgcttgcc 1261 tgtttattct tcctgtctta ctcagttatg ttctttggca tcactatgca ctaaatacat 1321 ggttgtttgc agttacagca ttttgtgtgg aactgtgctt aaaagtaatt gtttctctca 1381 ctgtttatac gttattcatg attgatggct actataatgt cctctgggaa aagcttgacg 1441 attatgtcta ctacgttcgt tcaacaggca gtattattga atttatattt ggagttgtaa 1501 tgtttggaaa tggggcttac actatgatgt ttgagtcggg aagtaaaatt cgggctttta 1561 tgatgtgcct acatgcatat tttaacatct acttacaagc caaaaatggc tggaagacat 1621 ttatgaatcg taggactgct gtgaagaaaa ttaattcact tcctgaaata aaagggagcc 1681 gcttacaaga aataaatgat gtatgtgcaa tctgctatca tgagtttaca acatctgctc 1741 gtattacacc gtgtaatcat tatttccatg cactttgcct tcggaaatgg ctgtacattc 1801 aagatacttg tccaatgtgc catcagaaag tatacatcga agatgatatc aaggataatt 1861 caaatgtatc taacaacaat ggatttattc cacccaatga aactccagag gaagctgtaa 1921 gagaagctgc tgctgaatct gacagggaat tgaacgaaga tgacagtaca gattgtgatg 1981 atgatgttca aagagaaaga aatggagtga ttcagcacac aggcgcagca gctgaagaat 2041 ttaatgatga tactgactgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 2101 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2161 agtcgacaat caacctctgg atta