Construct: ORF ccsbBroad304_07789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016810.1_s304c1, BRDN0000402818
- Derived from:
- ccsbBroadEn_07789
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PXMP4 (11264)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07789
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11264 | PXMP4 | peroxisomal membrane protein 4 | NM_007238.5 | 99.8% | 99.5% | 610G>A |
| 2 | human | 11264 | PXMP4 | peroxisomal membrane protein 4 | NM_183397.3 | 33.4% | 28.7% | 173_174ins199;213_214ins224 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agccccgccg cagctaaggg ctctgctcgt agtcgtcaac gcactgctgc 121 gcaagcgccg ctaccacgct gcgttggccg tgcttaaggg cttccggaac ggggctgtct 181 atggagccaa aatccgggcc cctcacgcgc tggtcatgac ctttctcttc cggaatggca 241 gcctccagga gaagctgtgg gccatactgc aggccacata tatccactcc tggaacctgg 301 cacggtttgt gttcacctac aagggtctcc gtgccctgca gtcctacata caaggcaaga 361 cctacccagc acacgcattc ctggcggcct tcctcggggg tatcctggtg tttggagaaa 421 acaataacat caacagccag atcaacatgt acctgttgtc acgcgtcctg tttgccctga 481 gccgcctggc tgtagagaag ggctacatcc ctgaacccag gtgggacccg ttcccgctgc 541 tcactgcggt ggtgtggggg ctggtgctgt ggctctttga gtatcaccga tccaccctgc 601 agccctcgct gcagtcctcc atgacctacc tctatgagga cagcaatgta tggcacgaca 661 tctcagactt cctcatctat aacaagagcc gtccctccaa ttacccaact ttcttgtaca 721 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 781 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta