Construct: ORF ccsbBroad304_07861

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016611.1_s304c1, BRDN0000462319
Derived from:
ccsbBroadEn_07861
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COBL (23242)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07861
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23242 COBL cordon-bleu WH2 repeat protein NM_015198.5 99.9% 100% 213T>C
2 human 23242 COBL cordon-bleu WH2 repeat protein XM_017011898.1 98.7% 98.8% 213T>C;684_728del
3 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515237.1 96.8% 96.9% 213T>C;684_728del;829_903del
4 human 23242 COBL cordon-bleu WH2 repeat protein NM_001346442.2 96.2% 96.2% 213T>C;3502_3503ins141
5 human 23242 COBL cordon-bleu WH2 repeat protein XM_005271751.1 95.6% 95.5% 213T>C;1097_1267del
6 human 23242 COBL cordon-bleu WH2 repeat protein XM_005271750.1 94.5% 94.5% 213T>C;684_728del;1142_1312del
7 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515240.1 94.3% 94.4% 213T>C;784_858del;3577_3578ins141
8 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515235.1 93.8% 93.8% 213T>C;784_858del;1172_1342del
9 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515239.1 93.2% 93.3% (many diffs)
10 human 23242 COBL cordon-bleu WH2 repeat protein NM_001346443.2 92.8% 92.7% (many diffs)
11 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515234.1 92.8% 92.7% (many diffs)
12 human 23242 COBL cordon-bleu WH2 repeat protein NM_001287436.2 92% 92% 213T>C;1097_1267del;3673_3674ins141
13 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515236.1 89.3% 89.3% (many diffs)
14 human 23242 COBL cordon-bleu WH2 repeat protein NM_001346441.2 88.8% 88.6% (many diffs)
15 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515238.1 86.2% 86% (many diffs)
16 human 23242 COBL cordon-bleu WH2 repeat protein XM_011515241.1 75.1% 75% 0_1ins813;284_454del
17 human 23242 COBL cordon-bleu WH2 repeat protein NM_001346444.1 34.3% 29.4% (many diffs)
18 human 23242 COBL cordon-bleu WH2 repeat protein NM_001287438.3 29.5% 29% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3852
ORF length:
3783
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgcgccg cgcgcctcgg cggccaagcc cccgaccggg aggaagatga
121aggctcgtgc tcccccacct cctggaaagg ctgccactct gcatgtgcac agtgaccaga
181agccccccca cgatggggcc ctcgggtcgc agcagaactt ggttcgcatg aaggaggcgc
241tgagggccag caccatggac gtcaccgtgg tcctgcctag cgggctggag aagaggagcg
301tgctcaatgg gagccatgcg atgatggacc tactggttga actttgcctt cagaaccacc
361tgaatccatc ccaccatgcc cttgaaattc ggtcttcaga aacccaacaa cctttgagtt
421ttaagccaaa tactttgatt gggaccctga atgtgcatac tgtgtttctg aaagaaaaag
481ttcctgaaga gaaggttaag cctggtcccc ctaaggtgcc tgagaaatct gtgcgtttgg
541tcgtgaatta cctgcggaca caaaaagctg ttgtgcgtgt gagccctgag gttcctctcc
601agaatattct cccagtcatt tgtgcaaagt gtgaggtcag cccagagcac gtggttctcc
661tcagggacaa cattgccgga gaggagctgg agctgtccaa gtccctgaac gagctcggga
721taaaggagct ctacgcgtgg gacaacagaa gagaaacctt taggaaatca tcacttggca
781atgatgagac agataaagag aagaaaaaat ttctgggatt tttcaaagtt aataaaagaa
841gcaatagtaa gggctgttta acgaccccca actccccatc catgcactca cgttctctta
901cgctgggtcc atccctctcg ctgggcagca tctcaggggt gtccgtgaag tcggagatga
961agaagcgccg agcccctcct cctccaggtt cagggccacc tgtgcaagac aaggcatcgg
1021aaaaggtatc tcttgggtca cagattgatt tacagaagaa gaagcggcga gcgccagctc
1081cccctccacc acagccacca ccaccgagtc ccctgatccc caaccgcact gaggataagg
1141aggagaacag gaagagcacg atggtaagcc tgcccctggg gtctggcagc cactgcagcc
1201cggatggagc cccgcaggtg ctgtcagagg cggaggagac cgtgtcagtt ggcagctgtt
1261ttgcgtcgga ggacacgacc gaggactcag gagtgatgag ttccccctca gacatcgtct
1321ctctggactc gcagcaggac agcatgaaat acaaagacaa gtgggccaca gaccaggaag
1381actgcagtga ccaggacctc gctggaaccc cagacctggg tccccagaag agccccttgt
1441gggagaagaa tggctctgag aactcacatc tgagaactga aaaagctgtc acagcctcaa
1501atgatgaaga agacctatta attgctggag agttccgtaa aacccttgca gaacttgatg
1561aagacctgga agaaatggaa gacagctatg aaacagacac cagctccctc accagctcca
1621tccatggtgc atccaaccac tgcccacagg atgccatgat ccctcacggc gacacagatg
1681caatcccagt aacattcata ggggaagttt cagatgatcc tgtggattcg gggttgtttt
1741ccaatagaaa caacaatgct gggtctttcg actcggaggg tgttgccagc aggagagact
1801ccctggcgcc acttcaagct gagcacagcc agccccatga aaaggcaagg gaggaagtac
1861ctgccctgca ccccgcttcc catgacgtcg gaaaaggaat ccgtgtggcc ttatctaaca
1921tctctaaaga tgggaatcta atggaaacgg cgcccagggt gacttctttt gcttcgaatc
1981ttcacacaga caacctaaat gcaaaagtga aagacaaagt gtatggctgt gctgacgggg
2041agaggactca agccacagag agagtgaatt cccaaccggt gaatgaaaag gacagcaacg
2101ataaaaacgc tgccttggca ccaacatcat ggcaccaacg aggccaaaac ccagggaaaa
2161gctacagact taagcacggc ctcacaacgt ataaaatcat tcctccaaag tcagagatgc
2221gatgttacga cagagatgtg tccctctcca ccggagccat taagattgac gagctgggga
2281acttggtgag tcctcacgcc accggcatca ggatcatttc cctgtcttcg tctgtgcctg
2341aagcagaatc tcagcccatt gggaaagtca gagagttctg gaggtgcaac tctgtggaaa
2401aacacctggg ccgaccctca gagagctctg ccaggggacc cccctccacc cctgtgccca
2461cgcagacgca gaatccagag agcagactcc aagcagaccc caagccaata tcgccccagc
2521agaagtctgc ccaccatgag ggccggaacc ccctagggga ggggagaaac cagcccccca
2581ccatgggcat gggtcacgtg agggtgccag cagctcacac cacagaagtc acatttctca
2641agcctcagag aagaacgtcc agccagtatg tggcctctgc cattgccaag cgcataggag
2701ccccaaaagt ccatgctgat gtggtgaggc cacacggtta tgccgagaag ggttatgcag
2761gcaaggcgcc agtgctggct gcaccacctg tcactgtgaa agatgacagg acatccagcc
2821cacactcaga gacacaagga tggaaggatg gtgcccagtg gccctgtgtc actcctccca
2881acaaccacgg ggaagatttg gcagtgggag cccctcctag gggggaggtc attggcccac
2941acaggaagtt gtctactcag gacagacctg ctgctatcca cagaagctcc tgtttctcac
3001tggttcagtc ttcccagagg gatcgtgttt ctgtgggaca gagctgtggt ttcagtggaa
3061agcaaagcac aagtagccag gaggccagct cagcatctga gcccagacgc gcacctgatg
3121gtacagaccc accccctcca cacacatctg acactcaggc ctgcagcagg gagctggtca
3181atggctctgt gcgcgcccca ggccacggcg agccttccca ccctccagga gggtctggca
3241cagaaagcca cattctccta gaaagagagg aaaagcccag tgtgttctct acagatggaa
3301atgaaacaga cagtatttgg ccacccagca tttttgggcc gaagaaaaaa ttcaaacctg
3361ttgtccagag accagtccca aaagacacat ccctgcactc tgccctgatg gaagccatcc
3421actctgcggg agggaaggac agactacgca agacggcaga acacaccggg gagggcaggc
3481cagcgaaact gtcctacacg gaggcagagg gcgaacgatc tgcactgctg gcagctatcc
3541gcgggcacag cggcacctgc agcctgagga aggtggcatc ctctgcttct gaggagctcc
3601agagcttccg agatgccgca ctctctgctc agggctcgga aagtcctctg ctagaagacc
3661ttggtcttct gtccccacca gccattcccc caccaccccc tccaccctcc caggctctct
3721ctgcaccaag gacggcctcc aggttcagca cgggcaccct cagcaacacc gcagacgcaa
3781ggcaagcctt gatggacgcc atccgctccg gcacaggggc tgcgagactg agaaaggtgc
3841ccttgctcgt gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
3901tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
3961caatcaacct ctggatta

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