Construct: ORF ccsbBroad304_07874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006082.1_s304c1, BRDN0000462275
- Derived from:
- ccsbBroadEn_07874
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMG5 (23381)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07874
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23381 | SMG5 | SMG5 nonsense mediated mRNA... | NM_015327.3 | 99.9% | 99.8% | 8A>C;3010A>G |
| 2 | human | 23381 | SMG5 | SMG5 nonsense mediated mRNA... | NM_001323615.1 | 95.4% | 95.2% | 8A>C;1114_1115ins138;2872A>G |
| 3 | human | 23381 | SMG5 | SMG5 nonsense mediated mRNA... | NM_001323614.1 | 94.7% | 94.5% | 8A>C;2282_2283ins159;2851A>G |
| 4 | human | 23381 | SMG5 | SMG5 nonsense mediated mRNA... | NM_001323616.1 | 93.4% | 93.4% | 0_1ins198;2812A>G |
| 5 | human | 23381 | SMG5 | SMG5 nonsense mediated mRNA... | NM_001323617.1 | 93.4% | 93.4% | 0_1ins198;2812A>G |
| 6 | mouse | 229512 | Smg5 | Smg-5 homolog, nonsense med... | NM_178246.3 | 88.4% | 93% | (many diffs) |
| 7 | mouse | 229512 | Smg5 | Smg-5 homolog, nonsense med... | XM_006501343.3 | 87% | 91.6% | (many diffs) |
| 8 | mouse | 229512 | Smg5 | Smg-5 homolog, nonsense med... | XM_011240087.2 | 82.6% | 86.9% | (many diffs) |
| 9 | mouse | 229512 | Smg5 | Smg-5 homolog, nonsense med... | XM_006501345.2 | 65.1% | 67.9% | (many diffs) |
| 10 | mouse | 229512 | Smg5 | Smg-5 homolog, nonsense med... | XM_006501346.3 | 56.9% | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3114
- ORF length:
- 3048
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cccaggcccc cccacagggg agagcagcga gcccgaagca aaagtcctcc 121 acactaagcg gctttaccgg gctgtggtgg aggctgtgca tcgacttgac ctcatccttt 181 gcaacaaaac tgcttatcaa gaagtattca aaccagaaaa cattagcctg aggaacaagc 241 tgcgtgagct ctgcgtcaag cttatgttcc tgcacccagt ggactatggg agaaaggctg 301 aggagctgct gtggagaaag gtatactatg aagttatcca gcttatcaag actaacaaaa 361 agcacatcca cagccggagc actttggaat gtgcctacag gacgcacctg gttgctggta 421 ttggcttcta ccagcatctc cttctctata tccagtccca ctaccagctg gaactgcagt 481 gctgcatcga ctggacccat gtcactgacc ccctcatagg atgcaagaag ccagtgtctg 541 cctcagggaa ggagatggat tgggcacaga tggcatgtca ccgatgtctg gtgtatctgg 601 gggatttgtc ccgatatcag aatgaattag ctggcgtaga taccgagctg ctagccgaga 661 gattttacta ccaagccctg tcagtagctc ctcagattgg aatgcccttc aatcagctgg 721 gcaccctggc aggcagcaag tactataatg tggaagccat gtattgctac ctgcgctgca 781 tccagtcaga agtgtccttt gagggagcct atgggaacct caagcggctg tatgacaagg 841 cagccaaaat gtaccaccaa ctgaagaagt gtgagactcg gaaactgtct cctggcaaaa 901 agcgatgtaa agacattaaa aggttgctag tgaactttat gtatctgcaa agcctcctac 961 agcccaaaag cagctccgtg gactcagagc tgacctcact ttgccagtca gtcctggagg 1021 acttcaacct ctgcctcttc tacctgccct cctcacccaa cctcagcctg gccagtgagg 1081 atgaggagga gtatgagagt ggatatgctt tcctcccgga ccttctcatc tttcaaatgg 1141 tcatcatctg ccttatgtgt gtgcacagct tggagagagc aggatccaag cagtacagtg 1201 cagccattgc cttcaccctg gccctctttt cccacctcgt caatcatgtc aacatacggc 1261 tgcaggctga gctggaagag ggcgagaatc ccgtcccggc attccagagt gatggcacag 1321 atgaaccaga gtccaaggaa cctgtggaga aagaggagga gccagatcct gagcctcctc 1381 ctgtaacacc ccaagtgggt gagggcagaa agagccgtaa gttctctcgc ctctcctgtc 1441 tccgccgtcg ccgccaccca cccaaagttg gtgatgacag tgacctgagt gaaggctttg 1501 aatcggactc aagccatgac tcagcccggg ccagtgaggg ctcagacagt ggctctgaca 1561 agagtcttga aggtggggga acggcctttg atgctgaaac agactcggaa atgaatagcc 1621 aggagtcccg atcagacttg gaagatatgg aggaagagga ggggacacgg tcaccaaccc 1681 tggagccccc tcggggcaga tcagaggctc ccgattccct caatggccca ctgggcccca 1741 gtgaggctag cattgccagc aatctacaag ccatgtccac ccagatgttc cagactaagc 1801 gctgcttccg actggccccc acctttagca acctgctcct ccagcccacc accaaccctc 1861 atacctcggc cagccacagg ccttgcgtca atggggatgt agacaagcct tcagagccag 1921 cctctgagga gggctctgag tcggagggga gtgagtccag tggacgctcc tgtcggaatg 1981 agcgcagcat ccaggagaag cttcaggtcc tgatggccga aggtctgctt cctgctgtga 2041 aagtcttcct ggactggctt cggaccaacc ccgacctcat catcgtgtgt gcgcagagct 2101 ctcaaagtct gtggaaccgc ctgtctgtgt tgctgaatct gttgcctgct gctggtgaac 2161 tccaggagtc tggcctggcc ttgtgtcctg aggtccaaga tcttcttgaa ggttgtgaac 2221 tgcctgacct cccctctagc cttctgctcc cagaggacat ggctcttcgt aacctgcccc 2281 cgctccgagc tgcccacaga cgctttaact ttgacacgga tcggcccctg ctcagcacct 2341 tagaggagtc agtggtgcgc atctgctgca tccgcagctt tggtcatttc atcgcccgcc 2401 tgcaaggcag catcctgcag ttcaacccag aggttggcat cttcgtcagc attgcccagt 2461 ctgagcagga gagcctgctg cagcaggccc aggcacagtt ccgaatggca caggaggaag 2521 ctcgtcggaa caggctcatg agagacatgg ctcagctacg acttcagctc gaagtgtctc 2581 agctggaggg cagcctgcag cagcccaagg cccagtcagc catgtctccc tacctcgtcc 2641 ctgacaccca ggccctctgc caccatctcc ctgtcatccg ccaactggcc accagtggcc 2701 gcttcattgt catcatccca aggacagtga tcgatggcct ggatttgctg aagaaggaac 2761 acccaggggc ccgggatggg attcggtacc tggaggcaga gtttaaaaaa ggaaacaggt 2821 acattcgctg ccagaaagag gtgggaaaga gctttgagcg gcataagctg aagaggcagg 2881 atgcagatgc ctggactctc tataagatcc tagacagctg caaacagctg actctggccc 2941 agggggcagg tgaggaggat ccgagtggca tggtgaccat catcacaggc cttccactgg 3001 acaaccccag cgtgctttca ggccccatgc aggcagccct gcaggccgct gcccacgcca 3061 gtgtggacat caaggatgtt ctggacttct acaagcagtg gaaggaaatt ggttgcccaa 3121 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 3181 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta