Construct: ORF ccsbBroad304_07894

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000861.1_s304c1, BRDN0000462287
Derived from:
ccsbBroadEn_07894
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MTREX (23517)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07894
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23517 MTREX Mtr4 exosome RNA helicase NM_015360.5 99.9% 99.9% 2698C>A;2916C>A
2 mouse 72198 Skiv2l2 superkiller viralicidic act... NM_028151.2 88.2% 97.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3195
ORF length:
3126
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggacgca ttcggagatg agctgttcag cgtgttcgag ggcgactcga
121ccactgcggc gggaaccaaa aaagacaagg aaaaggacaa ggggaaatgg aaggggcctc
181cagggtctgc agacaaggca gggaaacgtt ttgatggtaa attacaatca gaatcaacta
241ataatggaaa aaataagaga gatgtagatt tcgaaggtac agatgaaccc atttttggaa
301agaagcccag gatagaagag tcaataactg aagacttaag tttggcagac ctgatgccca
361gagtcaaggt acaatcagtt gaaactgttg aagggtgtac acatgaggtt gcacttcctg
421cagaagagga ttatttacca cttaaaccac gagttggaaa agctgctaag gaatacccgt
481tcattcttga tgcttttcaa agagaggcca ttcagtgtgt tgacaataat cagtctgttc
541tagtatctgc acatacctca gcgggaaaaa cagtatgcgc cgagtatgcc attgcattgg
601ccttaaggga aaagcagcgt gtaatattta ccagcccaat taaggctctg agtaaccaaa
661aataccgtga aatgtatgaa gaatttcaag atgttggttt gatgactggt gatgttacta
721ttaatcctac ggcatcttgt cttgttatga ccacagagat tttgagaagt atgctttaca
781gaggttccga agttatgaga gaagttgctt gggttatatt tgatgaaatt cattatatga
841gagattcaga acgtggtgta gtatgggaag aaactattat tttgcttcct gataacgtcc
901actatgtctt tctttcggct actattccaa atgcccgaca gtttgctgaa tggatttgcc
961atttacataa acagccttgt catgttattt acacagatta tcggcccact ccattgcaac
1021actacatttt tccagcaggg ggagatggcc tgcatcttgt ggttgatgaa aatggtgact
1081tcagagaaga taattttaat actgcaatgc aagtgcttcg agatgcaggt gatttggcca
1141aaggagacca gaaagggcgg aaaggaggaa caaaaggacc atcaaatgtt ttcaaaattg
1201tgaagatgat tatggaaaga aatttccaac ctgtgattat tttcagtttt agtaagaaag
1261attgtgaagc ctatgcactt caaatgacca aattagattt caacacagat gaagaaaaga
1321agatggttga agaagtattc agtaatgcaa ttgattgctt atccgatgaa gataaaaaac
1381tccctcaggt agaacatgta cttcctcttt tgaagagggg aattggtatt caccatggtg
1441gtttacttcc tattttgaaa gaaactatag aaattctctt ttctgaagga ttgataaagg
1501ccttatttgc cacggagacc tttgctatgg gaattaacat gccagctaga actgttttat
1561ttacaaatgc ccgcaaattt gatgggaagg atttccgatg gatttcttct ggtgaataca
1621ttcagatgtc tggtcgtgct ggaaggagag gaatggatga tagaggaatt gtaattctta
1681tggtagatga aaagatgagc ccaacaattg gaaaacaatt acttaagggc tccgctgatc
1741ctctaaatag tgctttccat ttgacctaca acatggtttt gaacttacta cgtgtagaag
1801aaattaatcc tgagtacatg ttggaaaaat ccttctacca gtttcagcat tatagagcaa
1861ttccaggagt agtagagaag gtaaagaatt cagaagaaca gtataataaa atagtaattc
1921ccaatgaaga aagtgtggtt atctattata agattagaca gcagcttgcc aaattgggta
1981aagaaattga agaatatatt cacaaaccaa aatactgctt accttttcta caaccaggtc
2041gtttggtaaa ggtaaagaat gaaggagatg actttggctg gggagtagtg gtgaatttct
2101caaaaaagtc aaatgttaag cctaactctg gtgaactgga tcctttgtat gtagtagaag
2161tacttctgcg ctgtagcaaa gagagcttga aaaattcagc tacagaagct gcaaaaccag
2221ctaaacctga tgagaaagga gagatgcagg ttgtcccagt tttggtgcat ctcctgtctg
2281ctatcagcag tgttaggctt tacattccta aagaccttcg gccggtggac aatagacaga
2341gtgttttaaa atcaatacag gaagttcaga aacgttttcc tgacggcatc cccttattag
2401accctattga tgatatgggc attcaagatc aagggctgaa aaaagtcatt cagaaagtag
2461aagcttttga gcatcgaatg tattctcatc cacttcacaa tgatccaaat ttggaaactg
2521tgtatacgct ttgtgaaaaa aaagcacaga ttgcaataga tattaaatct gcaaagcgag
2581aactgaagaa agcaagaaca gtcctacaaa tggatgaact caaatgtcgc aaacgtgttt
2641taagaaggtt gggatttgct acttcttctg atgtaataga gatgaaagga cgagtggctt
2701gtgagataag cagtgctgat gagctccttc taactgagat gatgtttaat ggccttttca
2761atgacatttc tgcagaacag gcaacagcat tattaagctg ctttgtgttt caagagaatt
2821ctagtgagat gcccaaatta acagaacaat tagcaggacc acttcgtcaa atgcaggaat
2881gtgctaaaag aattgcaaaa gtttcagcag aagccaaatt ggaaattgat gaggaaactt
2941atctaagctc atttaaacct cacttaatgg atgtagtata tacatgggca actggagcta
3001catttgccca tatctgcaaa atgacagatg tctttgaagg cagcataatt cgttgtatga
3061ggcgcctgga agaattgctt cgacaaatgt gtcaagcagc aaaagccatt ggaaacactg
3121agctggaaaa taaatttgca gaaggaatca ccaaaatcaa gagagatatt gtgtttgctg
3181ccagcctcta cttgttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
3241ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
3301cgacaatcaa cctctggatt a

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