Construct: ORF ccsbBroad304_07908

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016372.1_s304c1, BRDN0000406883
Derived from:
ccsbBroadEn_07908
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PADI4 (23569)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07908
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23569 PADI4 peptidyl arginine deiminase 4 NM_012387.3 99.6% 99.5% (many diffs)
2 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541150.1 90.3% 90.1% (many diffs)
3 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541151.1 84.6% 84.4% (many diffs)
4 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541152.1 72.9% 73% 0_1ins537;552G>A;612C>A
5 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541155.1 58.5% 57.5% (many diffs)
6 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541153.1 58.4% 57.8% (many diffs)
7 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541154.2 58.4% 57.8% (many diffs)
8 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541156.1 57.8% 57.6% (many diffs)
9 human 23569 PADI4 peptidyl arginine deiminase 4 XM_011541157.1 55.1% 55.2% 0_1ins891;198G>A;258C>A
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2055
ORF length:
1989
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccaggggaca ttgatccgtg tgaccccaga gcagcccacc catgccgtgt
121gtgtgctggg caccttgact cagcttgaca tctgcagctc tgcccctgag gactgcacgt
181ccttcagcat caacgcctcc ccaggggtgg tcgtggatat tgcccacagc cctccagcca
241agaagaaatc cacaggttcc tccacatggc ccctggaccc tggggtagag gtgaccctga
301cgatgaaagc ggccagtggt agcacaggcg accagaaggt tcagatttca tactacggac
361ccaagactcc accagtcaaa gctctactct acctcaccgc ggtggaaatc tccctgtgcg
421cagacatcac ccgcaccggc aaagtgaagc caaccagagc tgtgaaagat cagaggacct
481ggacctgggg cccttgtgga cagggtgcca tcctgctggt gaactgtgac agagacaatc
541tcgaatcttc tgccatggac tgcgaggatg atgaagtgct tgacagcgaa gacctgcagg
601acatgtcgct gatgaccctg agcacgaaga cccccaagga cttcttcaca aaccatacac
661tggtgctcca cgtggccagg tctgagatgg acaaagtgag ggtgtttcag gccacacggg
721gcaaactgtc ctccaagtgc agcgtagtct tgggtcccaa gtggccctct cactacctga
781tggtccccgg tggaaagcac aacatggact tctacgtgga ggccctcgct ttcccggaca
841ccgacttccc ggggctcatt accctcacca tctccctgct ggacacgtcc aacctggagc
901tccccgaggc tgtggtgttc caagacagcg tggtcttccg cgtggcgccc tggatcatga
961cccccaacac ccagcccccg caggaggtgt acgcgtgcag tatttttgaa aatgaggact
1021tcctgaagtc agtgactact ctggccatga aagccaagtg caagctgacc atctgccctg
1081aggaggagaa catggatgac cagtggatgc aggatgaaat ggagatcggc tacatccaag
1141ccccacacaa aacactgccc gtggtcttcg actctccaag gaacagaggc ctgaaggagt
1201ttcccatcaa acgagtgatg ggtccagatt ttggctatgt aactcgaggg ccccaaacag
1261ggggtatcag tggactggac tcctttggga acctggaagt gagcccccca gtcacagtca
1321ggggcaagga atacccgctg ggcaggattc tcttcgggga cagctgttat cccagcaatg
1381acagccggca gatgcaccag gccctgcagg acttcctcag tgcccagcag gtgcaggccc
1441ctgtgaagct ctattctgac tggctgtccg tgggccacgt ggacgagttc ctgagctttg
1501tgccagcacc cgacaggaag ggcttccggc tgctcctggc cagccccagg tcctgctaca
1561aactgttcca ggagcagcag aatgagggcc acggggaggc cctgctgttc gaagggatca
1621agaaaaaaaa acagcagaaa ataaagaaca ttctgtcaaa caagacattg agagaacata
1681attcatttgt ggagagatgc atcgactgga accgcgagct gctgaagcgg gagctgggcc
1741tggccgagag tgacatcatt gacatcccgc agctcttcaa gctcaaagag ttctctaagg
1801cggaagcttt tttccccaac atggtgaaca tgctggtgct agggaagcac ctgggcatcc
1861ccaagccctt cgggcccgtc atcaacggcc gctgctgcct ggaggagaag gtgtgttccc
1921tgctggagcc actgggcctc cagtgcacct tcatcaacga cttcttcacc taccacatca
1981ggcatgggga ggtgcactgc ggcaccaacg tgcgcagaaa gcccttctcc ttcaagtggt
2041ggaacatggt gccctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2101ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2161cgacaatcaa cctctggatt a

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