Construct: ORF ccsbBroad304_07958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015360.1_s304c1, BRDN0000402198
- Derived from:
- ccsbBroadEn_07958
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1AKMT3 (25895)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07958
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25895 | EEF1AKMT3 | EEF1A lysine methyltransfer... | NM_015433.3 | 99.8% | 100% | 12C>G |
| 2 | human | 25895 | EEF1AKMT3 | EEF1A lysine methyltransfer... | NM_206914.2 | 55.9% | 43.4% | (many diffs) |
| 3 | mouse | 100504608 | Mettl21b | methyltransferase like 21B | NM_001205036.1 | 83.5% | 82.7% | (many diffs) |
| 4 | mouse | 100504608 | Mettl21b | methyltransferase like 21B | XM_017313752.1 | 66.2% | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacccgggc ccagatcccg aatctgagtc ggaatcggtg ttcccgcggg 121 aggtcgggct ctttgcagac tcttactcgg agaagagcca gttctgtttc tgtgggcatg 181 tgctgaccat cacgcagaac tttgggtccc gcctcggggt ggcggcgcgc gtgtgggacg 241 cggccctgag cctgtgcaat tatttcgaga gtcaaaatgt ggatttccga ggcaagaagg 301 tgatcgaact gggtgcgggg acaggcatcg tggggatctt ggcagcgctg cagggggggg 361 atgttaccat cactgacctg cccctggccc tagaacagat ccagggcaac gtccaggcca 421 atgtgccagc tggaggccag gcccaggtgc gtgccttgtc ctgggggatt gaccatcatg 481 tcttccctgc aaactatgac ctggtgctgg gggctgatat cgtgtacctg gaacccacct 541 tccctctgct gctggggacc ctccaacacc tgtgcaggcc ccatggcacc atctatctgg 601 cctccaagat gagaaaggag catgggacag agagcttctt tcagcacctc ctgccccagc 661 atttccaact ggagctggct cagcgggatg aggatgaaaa tgtcaacatc tatagggcca 721 ggcacaggga accaagacct gcttgcccaa ctttcttgta caaagtggtt ggtaagccta 781 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 841 gcgttaagtc gacaatcaac ctctggatta